Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0264

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0263 and Psest_0264 are separated by 120 nucleotidesPsest_0264 and Psest_0265 are separated by 68 nucleotidesPsest_0265 and Psest_0266 are separated by 88 nucleotidesPsest_0266 and Psest_0267 are separated by 74 nucleotides Psest_0263: Psest_0263 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 279,350 to 281,278 _0263 Psest_0264: Psest_0264 - monofunctional biosynthetic peptidoglycan transglycosylase, at 281,399 to 282,127 _0264 Psest_0265: Psest_0265 - Uncharacterized small membrane protein, at 282,196 to 282,570 _0265 Psest_0266: Psest_0266 - thiamine biosynthesis protein ThiS, at 282,659 to 282,859 _0266 Psest_0267: Psest_0267 - Uncharacterized enzyme of thiazole biosynthesis, at 282,934 to 283,728 _0267 Position (kb) 281 282 283Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 280.660 kb on - strand, within Psest_0263at 280.660 kb on - strand, within Psest_0263at 280.728 kb on + strand, within Psest_0263at 280.729 kb on - strand, within Psest_0263at 280.866 kb on + strand, within Psest_0263at 280.867 kb on - strand, within Psest_0263at 280.867 kb on - strand, within Psest_0263at 280.946 kb on - strand, within Psest_0263at 281.118 kb on - strandat 281.244 kb on + strandat 281.245 kb on - strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.268 kb on + strandat 281.269 kb on - strandat 281.269 kb on - strandat 281.269 kb on - strandat 281.269 kb on - strandat 281.269 kb on - strandat 281.269 kb on - strandat 281.269 kb on - strandat 281.269 kb on - strandat 281.303 kb on - strandat 281.344 kb on - strandat 281.390 kb on + strandat 281.390 kb on + strandat 281.390 kb on + strandat 281.390 kb on + strandat 281.390 kb on + strandat 281.390 kb on + strandat 281.390 kb on + strandat 281.391 kb on - strandat 281.391 kb on - strandat 281.391 kb on - strandat 281.391 kb on - strandat 281.400 kb on + strandat 281.400 kb on + strandat 281.400 kb on + strandat 281.400 kb on + strandat 281.401 kb on - strandat 281.451 kb on + strandat 281.452 kb on - strandat 281.511 kb on + strand, within Psest_0264at 281.511 kb on + strand, within Psest_0264at 281.511 kb on + strand, within Psest_0264at 281.511 kb on + strand, within Psest_0264at 281.511 kb on + strand, within Psest_0264at 281.511 kb on + strand, within Psest_0264at 281.511 kb on + strand, within Psest_0264at 281.511 kb on + strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.512 kb on - strand, within Psest_0264at 281.556 kb on - strand, within Psest_0264at 281.673 kb on + strand, within Psest_0264at 281.673 kb on + strand, within Psest_0264at 281.673 kb on + strand, within Psest_0264at 281.673 kb on + strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.674 kb on - strand, within Psest_0264at 281.974 kb on + strand, within Psest_0264at 282.056 kb on + strandat 282.056 kb on + strandat 282.057 kb on - strandat 282.057 kb on - strandat 282.160 kb on + strandat 282.160 kb on + strandat 282.160 kb on + strandat 282.161 kb on - strandat 282.161 kb on - strandat 282.161 kb on - strandat 282.161 kb on - strandat 282.161 kb on - strandat 282.162 kb on + strandat 282.163 kb on - strandat 282.208 kb on + strandat 282.299 kb on - strand, within Psest_0265at 282.299 kb on - strand, within Psest_0265at 282.299 kb on - strand, within Psest_0265at 282.299 kb on - strand, within Psest_0265at 282.299 kb on - strand, within Psest_0265at 282.299 kb on - strand, within Psest_0265at 282.329 kb on - strand, within Psest_0265at 282.454 kb on + strand, within Psest_0265at 282.454 kb on + strand, within Psest_0265at 282.455 kb on - strand, within Psest_0265at 282.622 kb on - strandat 282.686 kb on + strand, within Psest_0266at 282.686 kb on + strand, within Psest_0266at 282.687 kb on - strand, within Psest_0266at 282.726 kb on + strand, within Psest_0266at 282.727 kb on - strand, within Psest_0266at 282.837 kb on + strand, within Psest_0266at 282.837 kb on + strand, within Psest_0266at 282.837 kb on + strand, within Psest_0266at 282.837 kb on + strand, within Psest_0266at 282.838 kb on - strand, within Psest_0266at 282.838 kb on - strand, within Psest_0266at 282.838 kb on - strand, within Psest_0266at 282.838 kb on - strand, within Psest_0266at 282.838 kb on - strand, within Psest_0266at 282.838 kb on - strand, within Psest_0266at 282.838 kb on - strand, within Psest_0266at 282.889 kb on + strandat 282.890 kb on - strandat 282.892 kb on - strandat 282.909 kb on + strandat 282.909 kb on + strandat 282.910 kb on - strandat 282.982 kb on + strandat 282.982 kb on + strandat 283.015 kb on + strand, within Psest_0267at 283.015 kb on + strand, within Psest_0267at 283.015 kb on + strand, within Psest_0267at 283.015 kb on + strand, within Psest_0267

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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280,660 - Psest_0263 0.68 -3.7
280,660 - Psest_0263 0.68 +0.9
280,728 + Psest_0263 0.71 -0.7
280,729 - Psest_0263 0.71 +2.2
280,866 + Psest_0263 0.79 +1.9
280,867 - Psest_0263 0.79 +2.1
280,867 - Psest_0263 0.79 +0.9
280,946 - Psest_0263 0.83 -1.3
281,118 - +0.3
281,244 + -2.7
281,245 - +0.5
281,268 + -2.9
281,268 + +0.9
281,268 + -1.5
281,268 + -1.9
281,268 + -1.9
281,268 + -1.1
281,268 + +1.7
281,268 + +1.3
281,268 + -1.7
281,268 + -1.7
281,268 + -0.9
281,269 - -2.6
281,269 - +0.4
281,269 - +1.9
281,269 - -1.2
281,269 - -2.0
281,269 - +1.1
281,269 - +0.5
281,269 - +1.1
281,303 - +0.9
281,344 - -2.4
281,390 + +2.3
281,390 + -1.4
281,390 + -2.9
281,390 + +0.3
281,390 + -2.1
281,390 + -0.3
281,390 + +1.1
281,391 - +0.0
281,391 - +0.0
281,391 - -0.9
281,391 - -0.1
281,400 + +0.1
281,400 + -1.7
281,400 + -2.3
281,400 + -0.7
281,401 - -0.7
281,451 + +0.8
281,452 - +1.9
281,511 + Psest_0264 0.15 -1.9
281,511 + Psest_0264 0.15 +0.6
281,511 + Psest_0264 0.15 +0.1
281,511 + Psest_0264 0.15 -1.7
281,511 + Psest_0264 0.15 -3.2
281,511 + Psest_0264 0.15 +0.8
281,511 + Psest_0264 0.15 -0.0
281,511 + Psest_0264 0.15 +1.3
281,512 - Psest_0264 0.16 +0.5
281,512 - Psest_0264 0.16 -1.1
281,512 - Psest_0264 0.16 +0.3
281,512 - Psest_0264 0.16 -0.1
281,512 - Psest_0264 0.16 +1.8
281,512 - Psest_0264 0.16 +0.4
281,512 - Psest_0264 0.16 +1.1
281,512 - Psest_0264 0.16 -3.2
281,512 - Psest_0264 0.16 +0.6
281,512 - Psest_0264 0.16 +0.4
281,512 - Psest_0264 0.16 +0.3
281,512 - Psest_0264 0.16 +2.1
281,512 - Psest_0264 0.16 -1.9
281,512 - Psest_0264 0.16 +1.8
281,512 - Psest_0264 0.16 +0.7
281,512 - Psest_0264 0.16 +1.1
281,512 - Psest_0264 0.16 +1.4
281,512 - Psest_0264 0.16 -0.5
281,512 - Psest_0264 0.16 +0.7
281,512 - Psest_0264 0.16 -3.5
281,512 - Psest_0264 0.16 -2.2
281,512 - Psest_0264 0.16 +0.6
281,512 - Psest_0264 0.16 -0.2
281,512 - Psest_0264 0.16 +0.2
281,512 - Psest_0264 0.16 +0.9
281,512 - Psest_0264 0.16 +0.6
281,512 - Psest_0264 0.16 -0.1
281,556 - Psest_0264 0.22 -0.9
281,673 + Psest_0264 0.38 -1.3
281,673 + Psest_0264 0.38 -0.5
281,673 + Psest_0264 0.38 -0.0
281,673 + Psest_0264 0.38 -1.5
281,674 - Psest_0264 0.38 +0.5
281,674 - Psest_0264 0.38 -2.3
281,674 - Psest_0264 0.38 -3.1
281,674 - Psest_0264 0.38 +1.1
281,674 - Psest_0264 0.38 +1.3
281,674 - Psest_0264 0.38 +0.2
281,674 - Psest_0264 0.38 +0.9
281,674 - Psest_0264 0.38 +0.5
281,974 + Psest_0264 0.79 -0.4
282,056 + +0.8
282,056 + -2.7
282,057 - +1.0
282,057 - +1.0
282,160 + +0.0
282,160 + +0.3
282,160 + -0.2
282,161 - +0.1
282,161 - -0.1
282,161 - +1.0
282,161 - -0.6
282,161 - +0.4
282,162 + -0.2
282,163 - +2.5
282,208 + +1.0
282,299 - Psest_0265 0.27 -4.3
282,299 - Psest_0265 0.27 -0.5
282,299 - Psest_0265 0.27 -1.0
282,299 - Psest_0265 0.27 +0.3
282,299 - Psest_0265 0.27 -0.2
282,299 - Psest_0265 0.27 +3.4
282,329 - Psest_0265 0.35 -1.2
282,454 + Psest_0265 0.69 -2.7
282,454 + Psest_0265 0.69 +0.2
282,455 - Psest_0265 0.69 -0.1
282,622 - +1.4
282,686 + Psest_0266 0.13 -0.1
282,686 + Psest_0266 0.13 -1.7
282,687 - Psest_0266 0.14 +0.3
282,726 + Psest_0266 0.33 +1.3
282,727 - Psest_0266 0.34 +0.6
282,837 + Psest_0266 0.89 +1.5
282,837 + Psest_0266 0.89 -2.2
282,837 + Psest_0266 0.89 -2.0
282,837 + Psest_0266 0.89 +0.0
282,838 - Psest_0266 0.89 -0.4
282,838 - Psest_0266 0.89 -0.1
282,838 - Psest_0266 0.89 -0.8
282,838 - Psest_0266 0.89 -1.9
282,838 - Psest_0266 0.89 -3.2
282,838 - Psest_0266 0.89 +1.5
282,838 - Psest_0266 0.89 -2.0
282,889 + -0.2
282,890 - +1.3
282,892 - +0.5
282,909 + -0.6
282,909 + -2.2
282,910 - -3.1
282,982 + +0.1
282,982 + -3.3
283,015 + Psest_0267 0.10 -0.4
283,015 + Psest_0267 0.10 +1.5
283,015 + Psest_0267 0.10 -1.6
283,015 + Psest_0267 0.10 -0.7

Or see this region's nucleotide sequence