Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2516 and Psest_2517 overlap by 1 nucleotides Psest_2517 and Psest_2518 overlap by 10 nucleotides Psest_2518 and Psest_2519 are separated by 29 nucleotides Psest_2519 and Psest_2520 are separated by 65 nucleotides
Psest_2516: Psest_2516 - cytochrome c oxidase, subunit I, at 2,693,672 to 2,696,197
_2516
Psest_2517: Psest_2517 - hypothetical protein, at 2,696,197 to 2,696,556
_2517
Psest_2518: Psest_2518 - Predicted membrane protein, at 2,696,547 to 2,697,392
_2518
Psest_2519: Psest_2519 - Predicted redox protein, regulator of disulfide bond formation, at 2,697,422 to 2,697,673
_2519
Psest_2520: Psest_2520 - Predicted SAM-dependent methyltransferase, at 2,697,739 to 2,698,788
_2520
Position (kb)
2696
2697
2698 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2695.603 kb on + strand, within Psest_2516 at 2695.697 kb on + strand, within Psest_2516 at 2695.698 kb on - strand, within Psest_2516 at 2695.724 kb on + strand, within Psest_2516 at 2695.724 kb on + strand, within Psest_2516 at 2695.725 kb on - strand, within Psest_2516 at 2695.725 kb on - strand, within Psest_2516 at 2695.725 kb on - strand, within Psest_2516 at 2695.725 kb on - strand, within Psest_2516 at 2695.725 kb on - strand, within Psest_2516 at 2695.725 kb on - strand, within Psest_2516 at 2695.816 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.860 kb on + strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2695.861 kb on - strand, within Psest_2516 at 2696.073 kb on - strand at 2696.093 kb on + strand at 2696.094 kb on - strand at 2696.123 kb on + strand at 2696.124 kb on - strand at 2696.126 kb on + strand at 2696.127 kb on - strand at 2696.127 kb on - strand at 2696.127 kb on - strand at 2696.153 kb on + strand at 2696.153 kb on + strand at 2696.153 kb on + strand at 2696.154 kb on - strand at 2696.154 kb on - strand at 2696.154 kb on - strand at 2696.203 kb on + strand at 2696.203 kb on + strand at 2696.203 kb on + strand at 2696.204 kb on - strand at 2696.204 kb on - strand at 2696.204 kb on - strand at 2696.222 kb on - strand at 2696.282 kb on - strand, within Psest_2517 at 2696.379 kb on + strand, within Psest_2517 at 2696.379 kb on + strand, within Psest_2517 at 2696.380 kb on - strand, within Psest_2517 at 2696.380 kb on - strand, within Psest_2517 at 2696.582 kb on + strand at 2696.582 kb on + strand at 2696.585 kb on + strand at 2696.586 kb on - strand at 2696.586 kb on - strand at 2696.706 kb on + strand, within Psest_2518 at 2696.707 kb on - strand, within Psest_2518 at 2696.707 kb on - strand, within Psest_2518 at 2696.942 kb on - strand, within Psest_2518 at 2696.992 kb on + strand, within Psest_2518 at 2696.993 kb on - strand, within Psest_2518 at 2696.993 kb on - strand, within Psest_2518 at 2697.020 kb on + strand, within Psest_2518 at 2697.020 kb on + strand, within Psest_2518 at 2697.020 kb on + strand, within Psest_2518 at 2697.021 kb on - strand, within Psest_2518 at 2697.021 kb on - strand, within Psest_2518 at 2697.021 kb on - strand, within Psest_2518 at 2697.033 kb on + strand, within Psest_2518 at 2697.033 kb on + strand, within Psest_2518 at 2697.033 kb on + strand, within Psest_2518 at 2697.034 kb on - strand, within Psest_2518 at 2697.034 kb on - strand, within Psest_2518 at 2697.034 kb on - strand, within Psest_2518 at 2697.163 kb on + strand, within Psest_2518 at 2697.166 kb on + strand, within Psest_2518 at 2697.166 kb on + strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.167 kb on - strand, within Psest_2518 at 2697.170 kb on + strand, within Psest_2518 at 2697.170 kb on + strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.171 kb on - strand, within Psest_2518 at 2697.300 kb on + strand, within Psest_2518 at 2697.301 kb on - strand, within Psest_2518 at 2697.301 kb on - strand, within Psest_2518 at 2697.783 kb on - strand at 2697.901 kb on + strand, within Psest_2520
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 2,695,603 + Psest_2516 0.76 -0.8 2,695,697 + Psest_2516 0.80 -0.7 2,695,698 - Psest_2516 0.80 -1.0 2,695,724 + Psest_2516 0.81 -0.4 2,695,724 + Psest_2516 0.81 +0.6 2,695,725 - Psest_2516 0.81 +0.1 2,695,725 - Psest_2516 0.81 -0.7 2,695,725 - Psest_2516 0.81 -3.2 2,695,725 - Psest_2516 0.81 +0.8 2,695,725 - Psest_2516 0.81 +0.9 2,695,725 - Psest_2516 0.81 -1.8 2,695,816 + Psest_2516 0.85 -0.2 2,695,860 + Psest_2516 0.87 +0.3 2,695,860 + Psest_2516 0.87 +0.5 2,695,860 + Psest_2516 0.87 -1.5 2,695,860 + Psest_2516 0.87 -2.5 2,695,860 + Psest_2516 0.87 -2.0 2,695,860 + Psest_2516 0.87 +1.5 2,695,860 + Psest_2516 0.87 -1.1 2,695,860 + Psest_2516 0.87 -0.5 2,695,861 - Psest_2516 0.87 +0.7 2,695,861 - Psest_2516 0.87 -0.2 2,695,861 - Psest_2516 0.87 +1.0 2,695,861 - Psest_2516 0.87 -2.9 2,695,861 - Psest_2516 0.87 +0.0 2,695,861 - Psest_2516 0.87 -2.5 2,695,861 - Psest_2516 0.87 -2.3 2,695,861 - Psest_2516 0.87 -2.7 2,695,861 - Psest_2516 0.87 +1.0 2,695,861 - Psest_2516 0.87 +0.0 2,695,861 - Psest_2516 0.87 -1.5 2,696,073 - -2.1 2,696,093 + +1.5 2,696,094 - +1.8 2,696,123 + -1.0 2,696,124 - -0.8 2,696,126 + +0.8 2,696,127 - -3.0 2,696,127 - -2.7 2,696,127 - +0.1 2,696,153 + -2.5 2,696,153 + -0.4 2,696,153 + +0.6 2,696,154 - -2.3 2,696,154 - -0.5 2,696,154 - -1.3 2,696,203 + -2.5 2,696,203 + +1.3 2,696,203 + -0.3 2,696,204 - -1.1 2,696,204 - +0.6 2,696,204 - -1.5 2,696,222 - -2.7 2,696,282 - Psest_2517 0.24 -4.0 2,696,379 + Psest_2517 0.51 -1.0 2,696,379 + Psest_2517 0.51 -1.4 2,696,380 - Psest_2517 0.51 -1.0 2,696,380 - Psest_2517 0.51 +0.1 2,696,582 + +0.3 2,696,582 + -0.8 2,696,585 + -0.5 2,696,586 - -0.4 2,696,586 - -1.1 2,696,706 + Psest_2518 0.19 -0.3 2,696,707 - Psest_2518 0.19 -0.6 2,696,707 - Psest_2518 0.19 -2.0 2,696,942 - Psest_2518 0.47 +1.6 2,696,992 + Psest_2518 0.53 -4.5 2,696,993 - Psest_2518 0.53 -1.2 2,696,993 - Psest_2518 0.53 +1.3 2,697,020 + Psest_2518 0.56 -2.3 2,697,020 + Psest_2518 0.56 -3.2 2,697,020 + Psest_2518 0.56 +1.2 2,697,021 - Psest_2518 0.56 +1.8 2,697,021 - Psest_2518 0.56 +3.4 2,697,021 - Psest_2518 0.56 -1.5 2,697,033 + Psest_2518 0.57 -3.3 2,697,033 + Psest_2518 0.57 +0.7 2,697,033 + Psest_2518 0.57 +0.2 2,697,034 - Psest_2518 0.58 -0.5 2,697,034 - Psest_2518 0.58 -0.2 2,697,034 - Psest_2518 0.58 -0.1 2,697,163 + Psest_2518 0.73 -1.4 2,697,166 + Psest_2518 0.73 +1.5 2,697,166 + Psest_2518 0.73 -0.5 2,697,167 - Psest_2518 0.73 -0.9 2,697,167 - Psest_2518 0.73 -1.0 2,697,167 - Psest_2518 0.73 +1.0 2,697,167 - Psest_2518 0.73 +0.9 2,697,167 - Psest_2518 0.73 -0.9 2,697,167 - Psest_2518 0.73 -2.3 2,697,167 - Psest_2518 0.73 -1.5 2,697,167 - Psest_2518 0.73 -1.8 2,697,167 - Psest_2518 0.73 +0.4 2,697,170 + Psest_2518 0.74 +0.0 2,697,170 + Psest_2518 0.74 +0.5 2,697,171 - Psest_2518 0.74 +0.6 2,697,171 - Psest_2518 0.74 -3.4 2,697,171 - Psest_2518 0.74 -2.9 2,697,171 - Psest_2518 0.74 +1.0 2,697,171 - Psest_2518 0.74 -0.2 2,697,171 - Psest_2518 0.74 +0.0 2,697,171 - Psest_2518 0.74 -0.2 2,697,171 - Psest_2518 0.74 -0.3 2,697,171 - Psest_2518 0.74 +0.2 2,697,300 + Psest_2518 0.89 -1.0 2,697,301 - Psest_2518 0.89 -0.5 2,697,301 - Psest_2518 0.89 -0.9 2,697,783 - -0.3 2,697,901 + Psest_2520 0.15 -0.2
Or see this region's nucleotide sequence