Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2279 and Psest_2280 are separated by 85 nucleotides Psest_2280 and Psest_2281 overlap by 20 nucleotides Psest_2281 and Psest_2282 overlap by 8 nucleotides Psest_2282 and Psest_2283 are separated by 102 nucleotides
Psest_2279: Psest_2279 - coenzyme PQQ biosynthesis protein E, at 2,427,544 to 2,428,689
_2279
Psest_2280: Psest_2280 - Predicted membrane protein, at 2,428,775 to 2,429,278
_2280
Psest_2281: Psest_2281 - Phosphoserine aminotransferase, at 2,429,259 to 2,430,359
_2281
Psest_2282: Psest_2282 - Dihydroorotate dehydrogenase, at 2,430,352 to 2,431,197
_2282
Psest_2283: Psest_2283 - Parvulin-like peptidyl-prolyl isomerase, at 2,431,300 to 2,433,156
_2283
Position (kb)
2429
2430
2431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2428.259 kb on - strand, within Psest_2279 at 2428.261 kb on + strand, within Psest_2279 at 2428.261 kb on + strand, within Psest_2279 at 2428.262 kb on - strand, within Psest_2279 at 2428.262 kb on - strand, within Psest_2279 at 2428.262 kb on - strand, within Psest_2279 at 2428.620 kb on + strand at 2428.621 kb on - strand at 2428.747 kb on - strand at 2428.846 kb on + strand, within Psest_2280 at 2428.846 kb on + strand, within Psest_2280 at 2428.846 kb on + strand, within Psest_2280 at 2428.846 kb on + strand, within Psest_2280 at 2428.847 kb on - strand, within Psest_2280 at 2428.847 kb on - strand, within Psest_2280 at 2428.847 kb on - strand, within Psest_2280 at 2429.040 kb on + strand, within Psest_2280 at 2429.040 kb on + strand, within Psest_2280 at 2429.041 kb on - strand, within Psest_2280 at 2429.041 kb on - strand, within Psest_2280 at 2429.189 kb on - strand, within Psest_2280 at 2429.292 kb on + strand at 2429.292 kb on + strand at 2429.293 kb on - strand at 2429.293 kb on - strand at 2429.458 kb on + strand, within Psest_2281 at 2429.478 kb on + strand, within Psest_2281 at 2429.479 kb on - strand, within Psest_2281 at 2429.479 kb on - strand, within Psest_2281 at 2429.776 kb on + strand, within Psest_2281 at 2429.848 kb on - strand, within Psest_2281 at 2429.848 kb on - strand, within Psest_2281 at 2429.890 kb on + strand, within Psest_2281 at 2429.890 kb on + strand, within Psest_2281 at 2429.891 kb on - strand, within Psest_2281 at 2429.891 kb on - strand, within Psest_2281 at 2429.897 kb on - strand, within Psest_2281 at 2429.953 kb on - strand, within Psest_2281 at 2429.953 kb on - strand, within Psest_2281 at 2430.045 kb on - strand, within Psest_2281 at 2430.126 kb on + strand, within Psest_2281 at 2430.126 kb on + strand, within Psest_2281 at 2430.272 kb on - strand at 2430.272 kb on - strand at 2430.347 kb on + strand at 2430.348 kb on - strand at 2430.357 kb on + strand at 2430.358 kb on - strand at 2430.479 kb on + strand, within Psest_2282 at 2430.479 kb on + strand, within Psest_2282 at 2430.722 kb on - strand, within Psest_2282 at 2430.866 kb on + strand, within Psest_2282 at 2430.939 kb on - strand, within Psest_2282
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 2,428,259 - Psest_2279 0.62 +1.3 2,428,261 + Psest_2279 0.63 -0.4 2,428,261 + Psest_2279 0.63 +0.8 2,428,262 - Psest_2279 0.63 +0.4 2,428,262 - Psest_2279 0.63 +1.0 2,428,262 - Psest_2279 0.63 -0.7 2,428,620 + -2.0 2,428,621 - -0.2 2,428,747 - -1.5 2,428,846 + Psest_2280 0.14 -2.7 2,428,846 + Psest_2280 0.14 -0.7 2,428,846 + Psest_2280 0.14 -0.0 2,428,846 + Psest_2280 0.14 +2.4 2,428,847 - Psest_2280 0.14 +2.0 2,428,847 - Psest_2280 0.14 +2.0 2,428,847 - Psest_2280 0.14 -4.0 2,429,040 + Psest_2280 0.53 -2.3 2,429,040 + Psest_2280 0.53 +0.7 2,429,041 - Psest_2280 0.53 -3.4 2,429,041 - Psest_2280 0.53 -0.3 2,429,189 - Psest_2280 0.82 -3.0 2,429,292 + -0.4 2,429,292 + +0.5 2,429,293 - -0.8 2,429,293 - +1.7 2,429,458 + Psest_2281 0.18 +1.3 2,429,478 + Psest_2281 0.20 -3.0 2,429,479 - Psest_2281 0.20 -2.7 2,429,479 - Psest_2281 0.20 -2.0 2,429,776 + Psest_2281 0.47 -1.7 2,429,848 - Psest_2281 0.53 -2.3 2,429,848 - Psest_2281 0.53 +1.0 2,429,890 + Psest_2281 0.57 +0.5 2,429,890 + Psest_2281 0.57 -0.2 2,429,891 - Psest_2281 0.57 -3.7 2,429,891 - Psest_2281 0.57 +2.1 2,429,897 - Psest_2281 0.58 -0.3 2,429,953 - Psest_2281 0.63 -3.3 2,429,953 - Psest_2281 0.63 -3.4 2,430,045 - Psest_2281 0.71 +1.1 2,430,126 + Psest_2281 0.79 -1.5 2,430,126 + Psest_2281 0.79 -0.9 2,430,272 - -2.6 2,430,272 - +1.0 2,430,347 + +0.0 2,430,348 - +0.8 2,430,357 + +2.4 2,430,358 - +0.6 2,430,479 + Psest_2282 0.15 +1.6 2,430,479 + Psest_2282 0.15 +0.4 2,430,722 - Psest_2282 0.44 +1.1 2,430,866 + Psest_2282 0.61 +2.0 2,430,939 - Psest_2282 0.69 +1.4
Or see this region's nucleotide sequence