Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2150 and Psest_2151 are separated by 289 nucleotides Psest_2151 and Psest_2152 are separated by 10 nucleotides Psest_2152 and Psest_2153 overlap by 4 nucleotides
Psest_2150: Psest_2150 - DNA polymerase LigD, polymerase domain, at 2,295,996 to 2,296,919
_2150
Psest_2151: Psest_2151 - formate dehydrogenase, alpha subunit, proteobacterial-type, at 2,297,209 to 2,300,274
_2151
Psest_2152: Psest_2152 - formate dehydrogenase, beta subunit, Fe-S containing, at 2,300,285 to 2,301,223
_2152
Psest_2153: Psest_2153 - formate dehydrogenase, gamma subunit, at 2,301,220 to 2,301,924
_2153
Position (kb)
2297
2298
2299
2300
2301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2296.821 kb on + strand, within Psest_2150 at 2297.176 kb on + strand at 2297.176 kb on + strand at 2297.176 kb on + strand at 2297.177 kb on - strand at 2297.358 kb on + strand at 2297.358 kb on + strand at 2297.358 kb on + strand at 2297.364 kb on + strand at 2297.364 kb on + strand at 2297.365 kb on - strand at 2297.365 kb on - strand at 2297.368 kb on + strand at 2297.368 kb on + strand at 2297.368 kb on + strand at 2297.368 kb on + strand at 2297.368 kb on + strand at 2297.369 kb on - strand at 2297.398 kb on + strand at 2297.398 kb on + strand at 2298.115 kb on + strand, within Psest_2151 at 2298.115 kb on + strand, within Psest_2151 at 2298.115 kb on + strand, within Psest_2151 at 2298.115 kb on + strand, within Psest_2151 at 2298.115 kb on + strand, within Psest_2151 at 2298.116 kb on - strand, within Psest_2151 at 2298.128 kb on - strand, within Psest_2151 at 2298.128 kb on - strand, within Psest_2151 at 2298.128 kb on - strand, within Psest_2151 at 2298.316 kb on + strand, within Psest_2151 at 2298.317 kb on - strand, within Psest_2151 at 2298.374 kb on + strand, within Psest_2151 at 2298.375 kb on - strand, within Psest_2151 at 2298.437 kb on - strand, within Psest_2151 at 2298.646 kb on + strand, within Psest_2151 at 2298.646 kb on + strand, within Psest_2151 at 2298.647 kb on - strand, within Psest_2151 at 2298.647 kb on - strand, within Psest_2151 at 2298.647 kb on - strand, within Psest_2151 at 2298.647 kb on - strand, within Psest_2151 at 2298.647 kb on - strand, within Psest_2151 at 2298.647 kb on - strand, within Psest_2151 at 2298.655 kb on + strand, within Psest_2151 at 2298.655 kb on + strand, within Psest_2151 at 2298.655 kb on + strand, within Psest_2151 at 2298.656 kb on - strand, within Psest_2151 at 2298.656 kb on - strand, within Psest_2151 at 2298.704 kb on - strand, within Psest_2151 at 2298.715 kb on + strand, within Psest_2151 at 2298.716 kb on - strand, within Psest_2151 at 2299.261 kb on + strand, within Psest_2151 at 2299.306 kb on + strand, within Psest_2151 at 2299.306 kb on + strand, within Psest_2151 at 2299.306 kb on + strand, within Psest_2151 at 2299.307 kb on - strand, within Psest_2151 at 2299.429 kb on + strand, within Psest_2151 at 2299.430 kb on - strand, within Psest_2151 at 2299.430 kb on - strand, within Psest_2151 at 2300.270 kb on + strand at 2300.271 kb on - strand at 2300.271 kb on - strand at 2300.271 kb on - strand at 2300.272 kb on + strand at 2300.272 kb on + strand at 2300.273 kb on - strand at 2300.273 kb on - strand at 2300.273 kb on - strand at 2300.273 kb on - strand at 2300.336 kb on - strand at 2300.486 kb on + strand, within Psest_2152 at 2300.486 kb on + strand, within Psest_2152 at 2300.486 kb on + strand, within Psest_2152 at 2300.486 kb on + strand, within Psest_2152 at 2300.486 kb on + strand, within Psest_2152 at 2300.486 kb on + strand, within Psest_2152 at 2300.487 kb on - strand, within Psest_2152 at 2300.487 kb on - strand, within Psest_2152 at 2300.487 kb on - strand, within Psest_2152 at 2300.655 kb on - strand, within Psest_2152 at 2300.741 kb on - strand, within Psest_2152 at 2300.766 kb on - strand, within Psest_2152 at 2300.766 kb on - strand, within Psest_2152 at 2300.876 kb on + strand, within Psest_2152 at 2300.877 kb on - strand, within Psest_2152 at 2300.877 kb on - strand, within Psest_2152 at 2300.933 kb on + strand, within Psest_2152 at 2300.933 kb on + strand, within Psest_2152 at 2300.933 kb on + strand, within Psest_2152 at 2300.934 kb on - strand, within Psest_2152 at 2300.934 kb on - strand, within Psest_2152 at 2300.934 kb on - strand, within Psest_2152 at 2300.969 kb on + strand, within Psest_2152 at 2300.969 kb on + strand, within Psest_2152 at 2300.969 kb on + strand, within Psest_2152 at 2300.969 kb on + strand, within Psest_2152 at 2300.970 kb on - strand, within Psest_2152 at 2300.970 kb on - strand, within Psest_2152 at 2301.002 kb on + strand, within Psest_2152 at 2301.003 kb on - strand, within Psest_2152 at 2301.003 kb on - strand, within Psest_2152 at 2301.119 kb on + strand, within Psest_2152 at 2301.119 kb on + strand, within Psest_2152 at 2301.119 kb on + strand, within Psest_2152 at 2301.119 kb on + strand, within Psest_2152 at 2301.120 kb on - strand, within Psest_2152 at 2301.120 kb on - strand, within Psest_2152 at 2301.120 kb on - strand, within Psest_2152 at 2301.120 kb on - strand, within Psest_2152 at 2301.120 kb on - strand, within Psest_2152 at 2301.236 kb on + strand at 2301.236 kb on + strand at 2301.236 kb on + strand at 2301.237 kb on - strand at 2301.237 kb on - strand at 2301.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 2,296,821 + Psest_2150 0.89 -1.7 2,297,176 + -0.0 2,297,176 + +0.3 2,297,176 + +1.8 2,297,177 - +0.8 2,297,358 + +3.2 2,297,358 + -0.7 2,297,358 + +0.3 2,297,364 + -1.2 2,297,364 + +0.3 2,297,365 - +2.4 2,297,365 - -1.4 2,297,368 + +0.7 2,297,368 + +3.1 2,297,368 + +1.2 2,297,368 + +0.8 2,297,368 + +0.4 2,297,369 - -1.2 2,297,398 + -0.6 2,297,398 + +1.3 2,298,115 + Psest_2151 0.30 +0.9 2,298,115 + Psest_2151 0.30 -1.5 2,298,115 + Psest_2151 0.30 +2.3 2,298,115 + Psest_2151 0.30 -0.2 2,298,115 + Psest_2151 0.30 -1.9 2,298,116 - Psest_2151 0.30 -0.9 2,298,128 - Psest_2151 0.30 -1.9 2,298,128 - Psest_2151 0.30 +1.4 2,298,128 - Psest_2151 0.30 +0.4 2,298,316 + Psest_2151 0.36 -1.0 2,298,317 - Psest_2151 0.36 +1.9 2,298,374 + Psest_2151 0.38 -0.3 2,298,375 - Psest_2151 0.38 +0.9 2,298,437 - Psest_2151 0.40 +0.9 2,298,646 + Psest_2151 0.47 +0.8 2,298,646 + Psest_2151 0.47 -1.9 2,298,647 - Psest_2151 0.47 -1.7 2,298,647 - Psest_2151 0.47 +0.4 2,298,647 - Psest_2151 0.47 +2.1 2,298,647 - Psest_2151 0.47 -1.7 2,298,647 - Psest_2151 0.47 +0.2 2,298,647 - Psest_2151 0.47 +0.8 2,298,655 + Psest_2151 0.47 -0.1 2,298,655 + Psest_2151 0.47 +1.4 2,298,655 + Psest_2151 0.47 +0.1 2,298,656 - Psest_2151 0.47 +1.1 2,298,656 - Psest_2151 0.47 +2.7 2,298,704 - Psest_2151 0.49 +0.7 2,298,715 + Psest_2151 0.49 +1.0 2,298,716 - Psest_2151 0.49 +0.9 2,299,261 + Psest_2151 0.67 +1.1 2,299,306 + Psest_2151 0.68 +1.3 2,299,306 + Psest_2151 0.68 -0.9 2,299,306 + Psest_2151 0.68 +3.1 2,299,307 - Psest_2151 0.68 -0.6 2,299,429 + Psest_2151 0.72 -1.5 2,299,430 - Psest_2151 0.72 -1.0 2,299,430 - Psest_2151 0.72 +1.6 2,300,270 + +1.7 2,300,271 - +1.9 2,300,271 - -0.7 2,300,271 - -1.9 2,300,272 + +1.0 2,300,272 + +0.1 2,300,273 - +1.1 2,300,273 - -0.2 2,300,273 - -2.1 2,300,273 - +0.8 2,300,336 - +1.2 2,300,486 + Psest_2152 0.21 -2.2 2,300,486 + Psest_2152 0.21 +0.7 2,300,486 + Psest_2152 0.21 -1.5 2,300,486 + Psest_2152 0.21 +2.3 2,300,486 + Psest_2152 0.21 +0.5 2,300,486 + Psest_2152 0.21 +1.1 2,300,487 - Psest_2152 0.22 -1.7 2,300,487 - Psest_2152 0.22 +0.4 2,300,487 - Psest_2152 0.22 -3.6 2,300,655 - Psest_2152 0.39 +0.9 2,300,741 - Psest_2152 0.49 -2.2 2,300,766 - Psest_2152 0.51 -1.2 2,300,766 - Psest_2152 0.51 +1.9 2,300,876 + Psest_2152 0.63 -0.0 2,300,877 - Psest_2152 0.63 -0.1 2,300,877 - Psest_2152 0.63 -0.1 2,300,933 + Psest_2152 0.69 +1.1 2,300,933 + Psest_2152 0.69 -0.1 2,300,933 + Psest_2152 0.69 -0.1 2,300,934 - Psest_2152 0.69 -0.1 2,300,934 - Psest_2152 0.69 +1.6 2,300,934 - Psest_2152 0.69 -0.9 2,300,969 + Psest_2152 0.73 -1.5 2,300,969 + Psest_2152 0.73 +1.1 2,300,969 + Psest_2152 0.73 +0.3 2,300,969 + Psest_2152 0.73 +0.7 2,300,970 - Psest_2152 0.73 +1.1 2,300,970 - Psest_2152 0.73 -0.5 2,301,002 + Psest_2152 0.76 +1.1 2,301,003 - Psest_2152 0.76 +0.7 2,301,003 - Psest_2152 0.76 -1.1 2,301,119 + Psest_2152 0.89 +0.1 2,301,119 + Psest_2152 0.89 +0.1 2,301,119 + Psest_2152 0.89 +1.1 2,301,119 + Psest_2152 0.89 +0.5 2,301,120 - Psest_2152 0.89 -1.2 2,301,120 - Psest_2152 0.89 +0.1 2,301,120 - Psest_2152 0.89 +0.0 2,301,120 - Psest_2152 0.89 +0.6 2,301,120 - Psest_2152 0.89 +1.7 2,301,236 + +0.4 2,301,236 + +1.1 2,301,236 + +0.8 2,301,237 - +1.8 2,301,237 - +1.8 2,301,237 - +1.3
Or see this region's nucleotide sequence