Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2133 and Psest_2134 are separated by 475 nucleotides Psest_2134 and Psest_2135 are separated by 87 nucleotides Psest_2135 and Psest_2136 are separated by 271 nucleotides
Psest_2133: Psest_2133 - Uncharacterized protein conserved in bacteria, at 2,266,056 to 2,269,724
_2133
Psest_2134: Psest_2134 - amino acid carrier protein, at 2,270,200 to 2,271,543
_2134
Psest_2135: Psest_2135 - Peroxiredoxin, at 2,271,631 to 2,272,131
_2135
Psest_2136: Psest_2136 - Predicted homoserine dehydrogenase, at 2,272,403 to 2,273,635
_2136
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2269.380 kb on + strand at 2269.390 kb on + strand at 2269.626 kb on - strand at 2269.707 kb on + strand at 2269.768 kb on - strand at 2269.933 kb on - strand at 2269.976 kb on + strand at 2269.976 kb on + strand at 2269.977 kb on - strand at 2270.004 kb on + strand at 2270.005 kb on - strand at 2270.023 kb on - strand at 2270.060 kb on + strand at 2270.060 kb on + strand at 2270.060 kb on + strand at 2270.061 kb on - strand at 2270.179 kb on + strand at 2270.179 kb on + strand at 2270.179 kb on + strand at 2270.179 kb on + strand at 2270.179 kb on + strand at 2270.180 kb on - strand at 2270.180 kb on - strand at 2270.180 kb on - strand at 2270.180 kb on - strand at 2270.180 kb on - strand at 2270.180 kb on - strand at 2270.182 kb on - strand at 2270.188 kb on + strand at 2270.188 kb on + strand at 2270.188 kb on + strand at 2270.188 kb on + strand at 2270.188 kb on + strand at 2270.188 kb on + strand at 2270.188 kb on + strand at 2270.189 kb on - strand at 2270.189 kb on - strand at 2270.189 kb on - strand at 2270.189 kb on - strand at 2270.222 kb on + strand at 2270.226 kb on + strand at 2270.226 kb on + strand at 2270.361 kb on + strand, within Psest_2134 at 2270.361 kb on + strand, within Psest_2134 at 2270.362 kb on - strand, within Psest_2134 at 2270.362 kb on - strand, within Psest_2134 at 2270.362 kb on - strand, within Psest_2134 at 2270.362 kb on - strand, within Psest_2134 at 2270.362 kb on - strand, within Psest_2134 at 2270.362 kb on - strand, within Psest_2134 at 2270.532 kb on + strand, within Psest_2134 at 2270.532 kb on + strand, within Psest_2134 at 2270.532 kb on + strand, within Psest_2134 at 2270.532 kb on + strand, within Psest_2134 at 2270.533 kb on - strand, within Psest_2134 at 2270.536 kb on + strand, within Psest_2134 at 2270.536 kb on + strand, within Psest_2134 at 2270.536 kb on + strand, within Psest_2134 at 2270.536 kb on + strand, within Psest_2134 at 2270.536 kb on + strand, within Psest_2134 at 2270.536 kb on + strand, within Psest_2134 at 2270.536 kb on + strand, within Psest_2134 at 2270.537 kb on - strand, within Psest_2134 at 2270.537 kb on - strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.608 kb on + strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.609 kb on - strand, within Psest_2134 at 2270.895 kb on - strand, within Psest_2134 at 2271.095 kb on + strand, within Psest_2134 at 2271.095 kb on + strand, within Psest_2134 at 2271.297 kb on + strand, within Psest_2134 at 2271.317 kb on - strand, within Psest_2134 at 2271.317 kb on - strand, within Psest_2134 at 2271.317 kb on - strand, within Psest_2134 at 2271.526 kb on + strand at 2271.527 kb on - strand at 2271.537 kb on + strand at 2271.537 kb on + strand at 2271.537 kb on + strand at 2271.538 kb on - strand at 2271.538 kb on - strand at 2271.538 kb on - strand at 2271.873 kb on + strand, within Psest_2135 at 2271.874 kb on - strand, within Psest_2135 at 2271.980 kb on - strand, within Psest_2135 at 2271.980 kb on - strand, within Psest_2135 at 2271.980 kb on - strand, within Psest_2135 at 2272.169 kb on + strand at 2272.265 kb on + strand at 2272.268 kb on + strand at 2272.268 kb on + strand at 2272.268 kb on + strand at 2272.268 kb on + strand at 2272.269 kb on - strand at 2272.269 kb on - strand at 2272.333 kb on - strand at 2272.373 kb on - strand at 2272.402 kb on + strand at 2272.402 kb on + strand at 2272.402 kb on + strand at 2272.403 kb on - strand at 2272.403 kb on - strand at 2272.403 kb on - strand at 2272.408 kb on + strand at 2272.408 kb on + strand at 2272.408 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 2,269,380 + -1.0 2,269,390 + -2.1 2,269,626 - -3.0 2,269,707 + -1.1 2,269,768 - -0.9 2,269,933 - +0.3 2,269,976 + +0.3 2,269,976 + -1.7 2,269,977 - -0.9 2,270,004 + -0.9 2,270,005 - -2.7 2,270,023 - -0.4 2,270,060 + -1.9 2,270,060 + +1.8 2,270,060 + +1.0 2,270,061 - +1.7 2,270,179 + -3.2 2,270,179 + +0.5 2,270,179 + -0.2 2,270,179 + -2.1 2,270,179 + +1.2 2,270,180 - +0.8 2,270,180 - -1.7 2,270,180 - -0.4 2,270,180 - -4.5 2,270,180 - -0.8 2,270,180 - +0.1 2,270,182 - +4.5 2,270,188 + +0.3 2,270,188 + +1.5 2,270,188 + +0.4 2,270,188 + +0.7 2,270,188 + +1.1 2,270,188 + +1.6 2,270,188 + -0.4 2,270,189 - -3.5 2,270,189 - -0.8 2,270,189 - -3.9 2,270,189 - -0.9 2,270,222 + -1.4 2,270,226 + -3.5 2,270,226 + +0.8 2,270,361 + Psest_2134 0.12 +1.3 2,270,361 + Psest_2134 0.12 -1.0 2,270,362 - Psest_2134 0.12 -1.7 2,270,362 - Psest_2134 0.12 -2.6 2,270,362 - Psest_2134 0.12 -3.8 2,270,362 - Psest_2134 0.12 -1.4 2,270,362 - Psest_2134 0.12 -0.6 2,270,362 - Psest_2134 0.12 -2.6 2,270,532 + Psest_2134 0.25 -0.4 2,270,532 + Psest_2134 0.25 -1.6 2,270,532 + Psest_2134 0.25 -2.2 2,270,532 + Psest_2134 0.25 +1.5 2,270,533 - Psest_2134 0.25 -3.4 2,270,536 + Psest_2134 0.25 -2.1 2,270,536 + Psest_2134 0.25 +0.1 2,270,536 + Psest_2134 0.25 -2.7 2,270,536 + Psest_2134 0.25 -2.9 2,270,536 + Psest_2134 0.25 -1.7 2,270,536 + Psest_2134 0.25 -1.2 2,270,536 + Psest_2134 0.25 -3.7 2,270,537 - Psest_2134 0.25 -0.4 2,270,537 - Psest_2134 0.25 -0.5 2,270,608 + Psest_2134 0.30 -2.2 2,270,608 + Psest_2134 0.30 -1.4 2,270,608 + Psest_2134 0.30 -2.6 2,270,608 + Psest_2134 0.30 +0.2 2,270,608 + Psest_2134 0.30 -0.0 2,270,608 + Psest_2134 0.30 -0.9 2,270,608 + Psest_2134 0.30 -3.8 2,270,608 + Psest_2134 0.30 -0.2 2,270,608 + Psest_2134 0.30 +1.0 2,270,609 - Psest_2134 0.30 -2.8 2,270,609 - Psest_2134 0.30 +0.4 2,270,609 - Psest_2134 0.30 -3.3 2,270,609 - Psest_2134 0.30 -2.4 2,270,609 - Psest_2134 0.30 +0.5 2,270,609 - Psest_2134 0.30 -0.1 2,270,609 - Psest_2134 0.30 -0.2 2,270,609 - Psest_2134 0.30 -4.1 2,270,609 - Psest_2134 0.30 -1.4 2,270,609 - Psest_2134 0.30 -1.4 2,270,609 - Psest_2134 0.30 -3.3 2,270,895 - Psest_2134 0.52 -2.7 2,271,095 + Psest_2134 0.67 -1.2 2,271,095 + Psest_2134 0.67 -4.4 2,271,297 + Psest_2134 0.82 +0.8 2,271,317 - Psest_2134 0.83 +0.4 2,271,317 - Psest_2134 0.83 +0.6 2,271,317 - Psest_2134 0.83 -1.0 2,271,526 + -0.4 2,271,527 - +0.1 2,271,537 + -0.1 2,271,537 + +0.4 2,271,537 + -3.2 2,271,538 - +0.4 2,271,538 - -0.1 2,271,538 - -2.1 2,271,873 + Psest_2135 0.48 -0.2 2,271,874 - Psest_2135 0.49 +1.1 2,271,980 - Psest_2135 0.70 +1.2 2,271,980 - Psest_2135 0.70 +1.6 2,271,980 - Psest_2135 0.70 +0.2 2,272,169 + +2.0 2,272,265 + +2.3 2,272,268 + -1.7 2,272,268 + +0.9 2,272,268 + -2.0 2,272,268 + +0.8 2,272,269 - +1.7 2,272,269 - +0.3 2,272,333 - +0.9 2,272,373 - +1.1 2,272,402 + -2.6 2,272,402 + +3.5 2,272,402 + -0.9 2,272,403 - +1.3 2,272,403 - +1.7 2,272,403 - -2.4 2,272,408 + +0.4 2,272,408 + +2.2 2,272,408 + +2.1
Or see this region's nucleotide sequence