Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2071 and Psest_2072 are separated by 111 nucleotides Psest_2072 and Psest_2073 are separated by 37 nucleotides Psest_2073 and Psest_2074 are separated by 14 nucleotides Psest_2074 and Psest_2075 are separated by 83 nucleotides
Psest_2071: Psest_2071 - hydroxyacylglutathione hydrolase, at 2,200,316 to 2,201,095
_2071
Psest_2072: Psest_2072 - Methyltransferase domain., at 2,201,207 to 2,201,977
_2072
Psest_2073: Psest_2073 - Ribonuclease HI, at 2,202,015 to 2,202,470
_2073
Psest_2074: Psest_2074 - DNA polymerase III, epsilon subunit, Proteobacterial, at 2,202,485 to 2,203,210
_2074
Psest_2075: Psest_2075 - Arginine/lysine/ornithine decarboxylases, at 2,203,294 to 2,205,543
_2075
Position (kb)
2202
2203 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.055 kb on + strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.056 kb on - strand at 2201.132 kb on + strand at 2201.132 kb on + strand at 2201.133 kb on - strand at 2201.133 kb on - strand at 2201.357 kb on + strand, within Psest_2072 at 2201.357 kb on + strand, within Psest_2072 at 2201.358 kb on - strand, within Psest_2072 at 2201.358 kb on - strand, within Psest_2072 at 2201.656 kb on - strand, within Psest_2072 at 2201.675 kb on - strand, within Psest_2072 at 2201.747 kb on + strand, within Psest_2072 at 2201.747 kb on + strand, within Psest_2072 at 2201.840 kb on + strand, within Psest_2072 at 2201.840 kb on + strand, within Psest_2072 at 2201.840 kb on + strand, within Psest_2072 at 2201.840 kb on + strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.841 kb on - strand, within Psest_2072 at 2201.844 kb on + strand, within Psest_2072 at 2201.844 kb on + strand, within Psest_2072 at 2201.845 kb on - strand, within Psest_2072 at 2202.439 kb on + strand at 2202.448 kb on + strand at 2202.448 kb on + strand at 2202.468 kb on + strand at 2202.468 kb on + strand at 2203.261 kb on - strand at 2203.267 kb on + strand at 2203.268 kb on - strand at 2203.297 kb on + strand at 2203.298 kb on - strand at 2203.298 kb on - strand at 2203.298 kb on - strand at 2203.340 kb on + strand at 2203.340 kb on + strand at 2203.340 kb on + strand at 2203.340 kb on + strand at 2203.341 kb on - strand at 2203.341 kb on - strand at 2203.341 kb on - strand at 2203.370 kb on + strand at 2203.370 kb on + strand at 2203.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 2,201,055 + +0.6 2,201,055 + +2.2 2,201,055 + +1.8 2,201,055 + -0.4 2,201,055 + +0.4 2,201,055 + +1.0 2,201,055 + -2.2 2,201,055 + +1.4 2,201,055 + -0.9 2,201,055 + +3.5 2,201,055 + +1.0 2,201,055 + +3.1 2,201,056 - +1.7 2,201,056 - -2.7 2,201,056 - +0.7 2,201,056 - -2.7 2,201,056 - -4.3 2,201,056 - -3.3 2,201,056 - -0.3 2,201,056 - -2.7 2,201,056 - +1.0 2,201,056 - +2.4 2,201,056 - +0.0 2,201,056 - +1.3 2,201,056 - -0.5 2,201,056 - +0.8 2,201,056 - -0.1 2,201,056 - +0.2 2,201,132 + +1.6 2,201,132 + -2.3 2,201,133 - -0.7 2,201,133 - -3.1 2,201,357 + Psest_2072 0.19 -2.7 2,201,357 + Psest_2072 0.19 -3.4 2,201,358 - Psest_2072 0.20 +0.7 2,201,358 - Psest_2072 0.20 -0.3 2,201,656 - Psest_2072 0.58 +1.9 2,201,675 - Psest_2072 0.61 +0.7 2,201,747 + Psest_2072 0.70 -0.7 2,201,747 + Psest_2072 0.70 -4.0 2,201,840 + Psest_2072 0.82 +0.5 2,201,840 + Psest_2072 0.82 +0.4 2,201,840 + Psest_2072 0.82 +1.2 2,201,840 + Psest_2072 0.82 -4.0 2,201,841 - Psest_2072 0.82 -0.4 2,201,841 - Psest_2072 0.82 -0.6 2,201,841 - Psest_2072 0.82 -0.6 2,201,841 - Psest_2072 0.82 -0.0 2,201,841 - Psest_2072 0.82 -1.7 2,201,841 - Psest_2072 0.82 -1.9 2,201,841 - Psest_2072 0.82 -1.0 2,201,841 - Psest_2072 0.82 -1.3 2,201,844 + Psest_2072 0.83 +0.3 2,201,844 + Psest_2072 0.83 -1.4 2,201,845 - Psest_2072 0.83 -0.0 2,202,439 + +1.4 2,202,448 + +0.4 2,202,448 + -1.5 2,202,468 + +1.9 2,202,468 + -0.4 2,203,261 - -0.9 2,203,267 + +2.1 2,203,268 - -0.6 2,203,297 + -3.9 2,203,298 - -2.6 2,203,298 - -2.7 2,203,298 - +0.9 2,203,340 + +1.8 2,203,340 + +2.4 2,203,340 + -0.1 2,203,340 + +1.6 2,203,341 - -0.2 2,203,341 - -3.5 2,203,341 - -3.7 2,203,370 + +0.2 2,203,370 + -0.4 2,203,371 - +4.8
Or see this region's nucleotide sequence