Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0202

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0201 and Psest_0202 are separated by 131 nucleotidesPsest_0202 and Psest_0203 overlap by 20 nucleotides Psest_0201: Psest_0201 - PAS domain S-box, at 199,180 to 200,760 _0201 Psest_0202: Psest_0202 - diguanylate cyclase (GGDEF) domain, at 200,892 to 202,823 _0202 Psest_0203: Psest_0203 - Signal transduction histidine kinase, at 202,804 to 204,204 _0203 Position (kb) 200 201 202 203Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 200.181 kb on + strand, within Psest_0201at 200.182 kb on - strand, within Psest_0201at 200.182 kb on - strand, within Psest_0201at 200.243 kb on + strand, within Psest_0201at 200.395 kb on - strand, within Psest_0201at 200.640 kb on + strandat 200.829 kb on + strandat 200.829 kb on + strandat 200.829 kb on + strandat 200.829 kb on + strandat 200.829 kb on + strandat 200.833 kb on + strandat 200.833 kb on + strandat 201.024 kb on + strandat 201.024 kb on + strandat 201.024 kb on + strandat 201.024 kb on + strandat 201.024 kb on + strandat 201.024 kb on + strandat 201.024 kb on + strandat 201.024 kb on + strandat 201.025 kb on - strandat 201.147 kb on + strand, within Psest_0202at 201.147 kb on + strand, within Psest_0202at 201.147 kb on + strand, within Psest_0202at 201.148 kb on - strand, within Psest_0202at 201.148 kb on - strand, within Psest_0202at 201.148 kb on - strand, within Psest_0202at 201.178 kb on - strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.357 kb on + strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.358 kb on - strand, within Psest_0202at 201.360 kb on - strand, within Psest_0202at 201.360 kb on - strand, within Psest_0202at 201.492 kb on + strand, within Psest_0202at 201.531 kb on + strand, within Psest_0202at 201.532 kb on - strand, within Psest_0202at 201.532 kb on - strand, within Psest_0202at 201.549 kb on + strand, within Psest_0202at 201.549 kb on + strand, within Psest_0202at 201.570 kb on + strand, within Psest_0202at 201.570 kb on + strand, within Psest_0202at 201.570 kb on + strand, within Psest_0202at 201.570 kb on + strand, within Psest_0202at 201.570 kb on + strand, within Psest_0202at 201.570 kb on + strand, within Psest_0202at 201.570 kb on + strand, within Psest_0202at 201.571 kb on - strand, within Psest_0202at 201.571 kb on - strand, within Psest_0202at 201.571 kb on - strand, within Psest_0202at 201.571 kb on - strand, within Psest_0202at 201.603 kb on + strand, within Psest_0202at 201.603 kb on + strand, within Psest_0202at 201.603 kb on + strand, within Psest_0202at 201.603 kb on + strand, within Psest_0202at 201.756 kb on + strand, within Psest_0202at 201.757 kb on - strand, within Psest_0202at 202.209 kb on + strand, within Psest_0202at 202.209 kb on + strand, within Psest_0202at 202.348 kb on + strand, within Psest_0202at 202.728 kb on + strandat 202.728 kb on + strandat 202.728 kb on + strandat 202.728 kb on + strandat 202.729 kb on - strandat 202.729 kb on - strandat 202.953 kb on - strand, within Psest_0203at 202.953 kb on - strand, within Psest_0203at 203.068 kb on - strand, within Psest_0203

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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200,181 + Psest_0201 0.63 +1.3
200,182 - Psest_0201 0.63 +0.9
200,182 - Psest_0201 0.63 -0.3
200,243 + Psest_0201 0.67 -0.3
200,395 - Psest_0201 0.77 +3.3
200,640 + -1.7
200,829 + +0.3
200,829 + +1.3
200,829 + +0.7
200,829 + +0.3
200,829 + +2.5
200,833 + -1.0
200,833 + -0.3
201,024 + +0.9
201,024 + +0.8
201,024 + -0.7
201,024 + +1.3
201,024 + +0.9
201,024 + -0.7
201,024 + +1.3
201,024 + -0.5
201,025 - -1.3
201,147 + Psest_0202 0.13 -0.3
201,147 + Psest_0202 0.13 +1.5
201,147 + Psest_0202 0.13 +1.3
201,148 - Psest_0202 0.13 -0.3
201,148 - Psest_0202 0.13 +0.9
201,148 - Psest_0202 0.13 +2.3
201,178 - Psest_0202 0.15 +1.3
201,357 + Psest_0202 0.24 +1.0
201,357 + Psest_0202 0.24 -1.0
201,357 + Psest_0202 0.24 -0.3
201,357 + Psest_0202 0.24 +0.1
201,357 + Psest_0202 0.24 +0.3
201,357 + Psest_0202 0.24 -0.3
201,357 + Psest_0202 0.24 -0.5
201,357 + Psest_0202 0.24 +0.3
201,357 + Psest_0202 0.24 +1.3
201,358 - Psest_0202 0.24 +1.0
201,358 - Psest_0202 0.24 +1.3
201,358 - Psest_0202 0.24 +2.9
201,358 - Psest_0202 0.24 +2.3
201,358 - Psest_0202 0.24 +4.8
201,358 - Psest_0202 0.24 +1.3
201,358 - Psest_0202 0.24 +0.8
201,358 - Psest_0202 0.24 +2.3
201,358 - Psest_0202 0.24 +1.3
201,358 - Psest_0202 0.24 -0.7
201,358 - Psest_0202 0.24 -0.3
201,358 - Psest_0202 0.24 +2.6
201,358 - Psest_0202 0.24 -0.3
201,358 - Psest_0202 0.24 +0.6
201,358 - Psest_0202 0.24 +0.9
201,358 - Psest_0202 0.24 +1.4
201,358 - Psest_0202 0.24 +0.3
201,358 - Psest_0202 0.24 +0.3
201,360 - Psest_0202 0.24 +1.6
201,360 - Psest_0202 0.24 +1.7
201,492 + Psest_0202 0.31 -0.7
201,531 + Psest_0202 0.33 +1.7
201,532 - Psest_0202 0.33 -0.7
201,532 - Psest_0202 0.33 +3.0
201,549 + Psest_0202 0.34 -0.7
201,549 + Psest_0202 0.34 -1.0
201,570 + Psest_0202 0.35 +0.3
201,570 + Psest_0202 0.35 +0.9
201,570 + Psest_0202 0.35 -1.3
201,570 + Psest_0202 0.35 +1.3
201,570 + Psest_0202 0.35 +0.3
201,570 + Psest_0202 0.35 +1.3
201,570 + Psest_0202 0.35 +0.3
201,571 - Psest_0202 0.35 +2.2
201,571 - Psest_0202 0.35 +1.3
201,571 - Psest_0202 0.35 +1.3
201,571 - Psest_0202 0.35 -0.3
201,603 + Psest_0202 0.37 +0.2
201,603 + Psest_0202 0.37 +2.7
201,603 + Psest_0202 0.37 +0.6
201,603 + Psest_0202 0.37 +1.4
201,756 + Psest_0202 0.45 +1.3
201,757 - Psest_0202 0.45 +1.7
202,209 + Psest_0202 0.68 +1.3
202,209 + Psest_0202 0.68 +1.3
202,348 + Psest_0202 0.75 +1.3
202,728 + -1.0
202,728 + +1.0
202,728 + -0.3
202,728 + +0.1
202,729 - +0.7
202,729 - +1.3
202,953 - Psest_0203 0.11 +1.3
202,953 - Psest_0203 0.11 +0.7
203,068 - Psest_0203 0.19 +1.6

Or see this region's nucleotide sequence