Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2037

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2036 and Psest_2037 are separated by 120 nucleotidesPsest_2037 and Psest_2038 are separated by 28 nucleotidesPsest_2038 and Psest_2039 are separated by 52 nucleotides Psest_2036: Psest_2036 - seryl-tRNA synthetase, at 2,156,928 to 2,158,208 _2036 Psest_2037: Psest_2037 - uroporphyrin-III C-methyltransferase, at 2,158,329 to 2,159,723 _2037 Psest_2038: Psest_2038 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 2,159,752 to 2,160,417 _2038 Psest_2039: Psest_2039 - PQQ-dependent catabolism-associated CXXCW motif protein, at 2,160,470 to 2,161,033 _2039 Position (kb) 2158 2159 2160Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2158.209 kb on + strandat 2158.830 kb on - strand, within Psest_2037at 2159.343 kb on + strand, within Psest_2037at 2159.344 kb on - strand, within Psest_2037at 2159.472 kb on + strand, within Psest_2037at 2159.472 kb on + strand, within Psest_2037at 2159.472 kb on + strand, within Psest_2037at 2159.472 kb on + strand, within Psest_2037at 2159.472 kb on + strand, within Psest_2037at 2159.473 kb on - strand, within Psest_2037at 2159.473 kb on - strand, within Psest_2037at 2159.473 kb on - strand, within Psest_2037at 2159.473 kb on - strand, within Psest_2037at 2159.721 kb on + strandat 2159.721 kb on + strandat 2159.721 kb on + strandat 2160.044 kb on + strand, within Psest_2038at 2160.128 kb on + strand, within Psest_2038at 2160.128 kb on + strand, within Psest_2038at 2160.129 kb on - strand, within Psest_2038at 2160.132 kb on + strand, within Psest_2038at 2160.132 kb on + strand, within Psest_2038at 2160.145 kb on + strand, within Psest_2038at 2160.145 kb on + strand, within Psest_2038at 2160.145 kb on + strand, within Psest_2038at 2160.145 kb on + strand, within Psest_2038at 2160.146 kb on - strand, within Psest_2038at 2160.146 kb on - strand, within Psest_2038at 2160.328 kb on + strand, within Psest_2038at 2160.329 kb on - strand, within Psest_2038at 2160.413 kb on + strandat 2160.413 kb on + strandat 2160.413 kb on + strandat 2160.413 kb on + strandat 2160.413 kb on + strandat 2160.414 kb on - strandat 2160.414 kb on - strandat 2160.558 kb on + strand, within Psest_2039at 2160.559 kb on - strand, within Psest_2039at 2160.568 kb on + strand, within Psest_2039at 2160.569 kb on - strand, within Psest_2039at 2160.570 kb on + strand, within Psest_2039at 2160.630 kb on + strand, within Psest_2039at 2160.690 kb on + strand, within Psest_2039at 2160.691 kb on - strand, within Psest_2039

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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2,158,209 + +1.8
2,158,830 - Psest_2037 0.36 -3.6
2,159,343 + Psest_2037 0.73 -0.3
2,159,344 - Psest_2037 0.73 +0.2
2,159,472 + Psest_2037 0.82 -2.8
2,159,472 + Psest_2037 0.82 -1.7
2,159,472 + Psest_2037 0.82 -1.2
2,159,472 + Psest_2037 0.82 -3.6
2,159,472 + Psest_2037 0.82 -0.9
2,159,473 - Psest_2037 0.82 -3.2
2,159,473 - Psest_2037 0.82 -1.9
2,159,473 - Psest_2037 0.82 -3.0
2,159,473 - Psest_2037 0.82 -0.5
2,159,721 + +0.0
2,159,721 + +0.8
2,159,721 + -0.3
2,160,044 + Psest_2038 0.44 -2.2
2,160,128 + Psest_2038 0.56 +1.8
2,160,128 + Psest_2038 0.56 +0.1
2,160,129 - Psest_2038 0.57 +1.0
2,160,132 + Psest_2038 0.57 +0.9
2,160,132 + Psest_2038 0.57 +2.3
2,160,145 + Psest_2038 0.59 +1.7
2,160,145 + Psest_2038 0.59 +1.6
2,160,145 + Psest_2038 0.59 -0.3
2,160,145 + Psest_2038 0.59 +0.3
2,160,146 - Psest_2038 0.59 +1.1
2,160,146 - Psest_2038 0.59 +1.7
2,160,328 + Psest_2038 0.86 +1.4
2,160,329 - Psest_2038 0.87 -1.5
2,160,413 + +0.5
2,160,413 + -1.3
2,160,413 + -2.4
2,160,413 + -2.2
2,160,413 + -0.0
2,160,414 - +1.9
2,160,414 - -0.7
2,160,558 + Psest_2039 0.16 -1.5
2,160,559 - Psest_2039 0.16 -4.9
2,160,568 + Psest_2039 0.17 -0.7
2,160,569 - Psest_2039 0.18 +0.4
2,160,570 + Psest_2039 0.18 -0.1
2,160,630 + Psest_2039 0.28 +1.3
2,160,690 + Psest_2039 0.39 -2.8
2,160,691 - Psest_2039 0.39 +1.1

Or see this region's nucleotide sequence