Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2018 and Psest_2019 are separated by 76 nucleotides Psest_2019 and Psest_2020 are separated by 87 nucleotides Psest_2020 and Psest_2021 overlap by 4 nucleotides
Psest_2018: Psest_2018 - Uncharacterized conserved protein, at 2,136,821 to 2,137,987
_2018
Psest_2019: Psest_2019 - adenylosuccinate lyase, at 2,138,064 to 2,139,434
_2019
Psest_2020: Psest_2020 - Uncharacterized protein involved in purine metabolism, at 2,139,522 to 2,140,160
_2020
Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284
_2021
Position (kb)
2138
2139
2140 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2137.186 kb on + strand, within Psest_2018 at 2137.186 kb on + strand, within Psest_2018 at 2137.187 kb on - strand, within Psest_2018 at 2137.365 kb on + strand, within Psest_2018 at 2137.575 kb on + strand, within Psest_2018 at 2137.575 kb on + strand, within Psest_2018 at 2137.576 kb on - strand, within Psest_2018 at 2137.617 kb on + strand, within Psest_2018 at 2137.618 kb on - strand, within Psest_2018 at 2138.004 kb on + strand at 2138.005 kb on - strand at 2138.005 kb on - strand at 2138.007 kb on - strand at 2138.008 kb on + strand at 2138.009 kb on - strand at 2138.009 kb on - strand at 2139.483 kb on - strand at 2139.483 kb on - strand at 2139.483 kb on - strand at 2139.754 kb on + strand, within Psest_2020 at 2139.754 kb on + strand, within Psest_2020 at 2139.754 kb on + strand, within Psest_2020 at 2139.756 kb on + strand, within Psest_2020 at 2139.756 kb on + strand, within Psest_2020 at 2139.757 kb on - strand, within Psest_2020 at 2139.905 kb on - strand, within Psest_2020 at 2139.910 kb on - strand, within Psest_2020 at 2140.000 kb on + strand, within Psest_2020
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 2,137,186 + Psest_2018 0.31 +0.9 2,137,186 + Psest_2018 0.31 -1.2 2,137,187 - Psest_2018 0.31 +1.9 2,137,365 + Psest_2018 0.47 -2.8 2,137,575 + Psest_2018 0.65 -1.7 2,137,575 + Psest_2018 0.65 -3.2 2,137,576 - Psest_2018 0.65 -1.5 2,137,617 + Psest_2018 0.68 -2.3 2,137,618 - Psest_2018 0.68 -2.1 2,138,004 + -3.9 2,138,005 - +2.3 2,138,005 - -2.6 2,138,007 - +2.1 2,138,008 + -2.3 2,138,009 - +1.0 2,138,009 - +1.4 2,139,483 - +0.7 2,139,483 - -1.9 2,139,483 - +1.2 2,139,754 + Psest_2020 0.36 -0.9 2,139,754 + Psest_2020 0.36 -2.6 2,139,754 + Psest_2020 0.36 -1.3 2,139,756 + Psest_2020 0.37 +1.8 2,139,756 + Psest_2020 0.37 -1.7 2,139,757 - Psest_2020 0.37 +1.3 2,139,905 - Psest_2020 0.60 +2.4 2,139,910 - Psest_2020 0.61 -0.0 2,140,000 + Psest_2020 0.75 -3.3
Or see this region's nucleotide sequence