Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1947 and Psest_1948 are separated by 92 nucleotides Psest_1948 and Psest_1949 are separated by 356 nucleotides Psest_1949 and Psest_1950 are separated by 39 nucleotides
Psest_1947: Psest_1947 - dihydroorotate dehydrogenase, subfamily 2, at 2,063,694 to 2,064,725
_1947
Psest_1948: Psest_1948 - Ribosome modulation factor, at 2,064,818 to 2,065,033
_1948
Psest_1949: Psest_1949 - Predicted N6-adenine-specific DNA methylase, at 2,065,390 to 2,067,570
_1949
Psest_1950: Psest_1950 - D-alanyl-D-alanine carboxypeptidase, serine-type, PBP4 family, at 2,067,610 to 2,069,067
_1950
Position (kb)
2065
2066
2067
2068 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2064.465 kb on + strand, within Psest_1947 at 2064.653 kb on + strand at 2064.653 kb on + strand at 2064.657 kb on + strand at 2064.657 kb on + strand at 2064.657 kb on + strand at 2064.657 kb on + strand at 2064.658 kb on - strand at 2064.767 kb on - strand at 2064.847 kb on + strand, within Psest_1948 at 2064.848 kb on - strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2065.094 kb on + strand at 2065.103 kb on + strand at 2065.103 kb on + strand at 2065.104 kb on - strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.227 kb on - strand at 2065.251 kb on + strand at 2065.251 kb on + strand at 2065.500 kb on + strand at 2065.501 kb on - strand at 2065.782 kb on + strand, within Psest_1949 at 2065.783 kb on - strand, within Psest_1949 at 2065.821 kb on - strand, within Psest_1949 at 2066.013 kb on + strand, within Psest_1949 at 2066.024 kb on + strand, within Psest_1949 at 2066.024 kb on + strand, within Psest_1949 at 2066.025 kb on - strand, within Psest_1949 at 2066.105 kb on + strand, within Psest_1949 at 2066.105 kb on + strand, within Psest_1949 at 2066.105 kb on + strand, within Psest_1949 at 2066.106 kb on - strand, within Psest_1949 at 2066.106 kb on - strand, within Psest_1949 at 2066.540 kb on + strand, within Psest_1949 at 2066.599 kb on - strand, within Psest_1949 at 2066.599 kb on - strand, within Psest_1949 at 2066.732 kb on - strand, within Psest_1949 at 2066.732 kb on - strand, within Psest_1949 at 2066.800 kb on + strand, within Psest_1949 at 2067.000 kb on - strand, within Psest_1949 at 2067.000 kb on - strand, within Psest_1949 at 2067.020 kb on - strand, within Psest_1949 at 2067.112 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.207 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.261 kb on - strand, within Psest_1949 at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.644 kb on + strand at 2067.645 kb on - strand at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.873 kb on + strand, within Psest_1950 at 2067.874 kb on - strand, within Psest_1950 at 2067.874 kb on - strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.272 kb on - strand, within Psest_1950 at 2068.298 kb on + strand, within Psest_1950 at 2068.298 kb on + strand, within Psest_1950 at 2068.299 kb on - strand, within Psest_1950 at 2068.299 kb on - strand, within Psest_1950 at 2068.343 kb on - strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.423 kb on + strand, within Psest_1950
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 2,064,465 + Psest_1947 0.75 -0.5 2,064,653 + -1.0 2,064,653 + +1.5 2,064,657 + -0.9 2,064,657 + -1.6 2,064,657 + -2.4 2,064,657 + -0.8 2,064,658 - +0.8 2,064,767 - -2.4 2,064,847 + Psest_1948 0.13 -0.5 2,064,848 - Psest_1948 0.14 -2.8 2,064,971 + Psest_1948 0.71 +0.4 2,064,971 + Psest_1948 0.71 +2.6 2,064,971 + Psest_1948 0.71 +1.0 2,064,971 + Psest_1948 0.71 -0.9 2,064,971 + Psest_1948 0.71 +1.2 2,064,971 + Psest_1948 0.71 +1.9 2,064,971 + Psest_1948 0.71 +1.0 2,064,971 + Psest_1948 0.71 +0.6 2,064,971 + Psest_1948 0.71 -0.9 2,064,971 + Psest_1948 0.71 +0.5 2,064,971 + Psest_1948 0.71 +0.3 2,064,971 + Psest_1948 0.71 -3.2 2,064,971 + Psest_1948 0.71 -0.5 2,064,971 + Psest_1948 0.71 -3.1 2,064,971 + Psest_1948 0.71 +0.3 2,064,971 + Psest_1948 0.71 -1.5 2,064,971 + Psest_1948 0.71 -0.1 2,064,972 - Psest_1948 0.71 +1.6 2,064,972 - Psest_1948 0.71 +2.1 2,064,972 - Psest_1948 0.71 +1.3 2,064,972 - Psest_1948 0.71 +0.8 2,064,972 - Psest_1948 0.71 -2.0 2,064,972 - Psest_1948 0.71 +0.6 2,064,972 - Psest_1948 0.71 +0.3 2,064,972 - Psest_1948 0.71 -2.2 2,064,972 - Psest_1948 0.71 +2.0 2,064,972 - Psest_1948 0.71 +1.4 2,064,972 - Psest_1948 0.71 +1.9 2,064,972 - Psest_1948 0.71 -2.7 2,065,094 + -1.3 2,065,103 + +1.6 2,065,103 + +2.0 2,065,104 - -0.3 2,065,213 + +0.6 2,065,213 + +1.2 2,065,213 + -0.4 2,065,213 + -0.5 2,065,213 + +0.9 2,065,213 + +0.4 2,065,213 + -1.2 2,065,214 - -0.7 2,065,214 - -1.2 2,065,214 - +0.3 2,065,214 - +0.9 2,065,214 - +0.9 2,065,227 - +1.5 2,065,251 + +0.6 2,065,251 + -0.6 2,065,500 + +0.8 2,065,501 - +0.6 2,065,782 + Psest_1949 0.18 +1.6 2,065,783 - Psest_1949 0.18 +1.3 2,065,821 - Psest_1949 0.20 +2.6 2,066,013 + Psest_1949 0.29 +1.4 2,066,024 + Psest_1949 0.29 +0.5 2,066,024 + Psest_1949 0.29 -0.9 2,066,025 - Psest_1949 0.29 +1.3 2,066,105 + Psest_1949 0.33 -4.0 2,066,105 + Psest_1949 0.33 -0.2 2,066,105 + Psest_1949 0.33 +1.8 2,066,106 - Psest_1949 0.33 +1.4 2,066,106 - Psest_1949 0.33 -0.8 2,066,540 + Psest_1949 0.53 -1.0 2,066,599 - Psest_1949 0.55 -1.9 2,066,599 - Psest_1949 0.55 -3.9 2,066,732 - Psest_1949 0.62 +0.9 2,066,732 - Psest_1949 0.62 -2.7 2,066,800 + Psest_1949 0.65 -0.1 2,067,000 - Psest_1949 0.74 -1.7 2,067,000 - Psest_1949 0.74 +2.1 2,067,020 - Psest_1949 0.75 -3.0 2,067,112 + Psest_1949 0.79 -0.5 2,067,187 + Psest_1949 0.82 +0.5 2,067,187 + Psest_1949 0.82 -0.0 2,067,187 + Psest_1949 0.82 -0.0 2,067,207 + Psest_1949 0.83 +1.1 2,067,260 + Psest_1949 0.86 -2.4 2,067,260 + Psest_1949 0.86 -0.5 2,067,260 + Psest_1949 0.86 +1.8 2,067,260 + Psest_1949 0.86 +2.6 2,067,260 + Psest_1949 0.86 +1.6 2,067,260 + Psest_1949 0.86 +2.2 2,067,260 + Psest_1949 0.86 -2.7 2,067,260 + Psest_1949 0.86 -4.1 2,067,261 - Psest_1949 0.86 -0.9 2,067,424 + +2.7 2,067,424 + -1.4 2,067,424 + +1.5 2,067,424 + +3.7 2,067,424 + -1.1 2,067,424 + +1.4 2,067,644 + +1.9 2,067,645 - +1.7 2,067,819 + Psest_1950 0.14 +1.1 2,067,819 + Psest_1950 0.14 +0.1 2,067,819 + Psest_1950 0.14 +0.2 2,067,819 + Psest_1950 0.14 +1.9 2,067,820 - Psest_1950 0.14 -0.1 2,067,820 - Psest_1950 0.14 -1.2 2,067,820 - Psest_1950 0.14 +2.6 2,067,820 - Psest_1950 0.14 -4.1 2,067,820 - Psest_1950 0.14 -2.8 2,067,820 - Psest_1950 0.14 -0.1 2,067,820 - Psest_1950 0.14 +1.6 2,067,820 - Psest_1950 0.14 +1.6 2,067,820 - Psest_1950 0.14 +0.1 2,067,820 - Psest_1950 0.14 +2.6 2,067,873 + Psest_1950 0.18 +1.3 2,067,874 - Psest_1950 0.18 +0.5 2,067,874 - Psest_1950 0.18 +0.8 2,068,121 + Psest_1950 0.35 +0.6 2,068,121 + Psest_1950 0.35 +1.2 2,068,121 + Psest_1950 0.35 +0.8 2,068,121 + Psest_1950 0.35 -0.1 2,068,121 + Psest_1950 0.35 +1.7 2,068,121 + Psest_1950 0.35 -1.3 2,068,121 + Psest_1950 0.35 +0.5 2,068,121 + Psest_1950 0.35 +0.6 2,068,122 - Psest_1950 0.35 -0.7 2,068,122 - Psest_1950 0.35 -0.3 2,068,122 - Psest_1950 0.35 +0.7 2,068,122 - Psest_1950 0.35 +0.4 2,068,122 - Psest_1950 0.35 -1.2 2,068,122 - Psest_1950 0.35 +0.6 2,068,122 - Psest_1950 0.35 -0.0 2,068,272 - Psest_1950 0.45 +1.6 2,068,298 + Psest_1950 0.47 +1.5 2,068,298 + Psest_1950 0.47 -3.5 2,068,299 - Psest_1950 0.47 -2.2 2,068,299 - Psest_1950 0.47 -0.9 2,068,343 - Psest_1950 0.50 -1.8 2,068,376 + Psest_1950 0.53 -0.2 2,068,376 + Psest_1950 0.53 +1.4 2,068,376 + Psest_1950 0.53 +0.4 2,068,376 + Psest_1950 0.53 -0.1 2,068,376 + Psest_1950 0.53 +1.3 2,068,376 + Psest_1950 0.53 +1.6 2,068,377 - Psest_1950 0.53 +1.2 2,068,377 - Psest_1950 0.53 +0.2 2,068,377 - Psest_1950 0.53 +1.8 2,068,377 - Psest_1950 0.53 -2.5 2,068,377 - Psest_1950 0.53 +1.4 2,068,381 + Psest_1950 0.53 -1.0 2,068,381 + Psest_1950 0.53 +0.5 2,068,381 + Psest_1950 0.53 +1.4 2,068,381 + Psest_1950 0.53 +0.7 2,068,382 - Psest_1950 0.53 +0.4 2,068,382 - Psest_1950 0.53 +1.5 2,068,382 - Psest_1950 0.53 +0.5 2,068,382 - Psest_1950 0.53 +0.1 2,068,423 + Psest_1950 0.56 +0.8
Or see this region's nucleotide sequence