Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1880 and Psest_1881 are separated by 86 nucleotides Psest_1881 and Psest_1882 overlap by 4 nucleotides Psest_1882 and Psest_1883 are separated by 91 nucleotides Psest_1883 and Psest_1884 are separated by 142 nucleotides
Psest_1880: Psest_1880 - Predicted Fe-S-cluster oxidoreductase, at 1,991,311 to 1,991,682
_1880
Psest_1881: Psest_1881 - Nitroreductase, at 1,991,769 to 1,992,341
_1881
Psest_1882: Psest_1882 - Signal transduction histidine kinase, at 1,992,338 to 1,993,684
_1882
Psest_1883: Psest_1883 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 1,993,776 to 1,994,189
_1883
Psest_1884: Psest_1884 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 1,994,332 to 1,995,009
_1884
Position (kb)
1992
1993
1994 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1991.356 kb on + strand, within Psest_1880 at 1991.357 kb on - strand, within Psest_1880 at 1991.374 kb on + strand, within Psest_1880 at 1991.374 kb on + strand, within Psest_1880 at 1991.374 kb on + strand, within Psest_1880 at 1991.374 kb on + strand, within Psest_1880 at 1991.374 kb on + strand, within Psest_1880 at 1991.375 kb on - strand, within Psest_1880 at 1991.375 kb on - strand, within Psest_1880 at 1991.504 kb on + strand, within Psest_1880 at 1991.504 kb on + strand, within Psest_1880 at 1991.504 kb on + strand, within Psest_1880 at 1991.504 kb on + strand, within Psest_1880 at 1991.504 kb on + strand, within Psest_1880 at 1991.505 kb on - strand, within Psest_1880 at 1991.505 kb on - strand, within Psest_1880 at 1991.530 kb on + strand, within Psest_1880 at 1991.530 kb on + strand, within Psest_1880 at 1991.530 kb on + strand, within Psest_1880 at 1991.530 kb on + strand, within Psest_1880 at 1991.530 kb on + strand, within Psest_1880 at 1991.531 kb on - strand, within Psest_1880 at 1991.531 kb on - strand, within Psest_1880 at 1991.531 kb on - strand, within Psest_1880 at 1991.531 kb on - strand, within Psest_1880 at 1991.531 kb on - strand, within Psest_1880 at 1991.738 kb on + strand at 1991.738 kb on + strand at 1991.763 kb on + strand at 1992.199 kb on + strand, within Psest_1881 at 1992.199 kb on + strand, within Psest_1881 at 1992.199 kb on + strand, within Psest_1881 at 1992.199 kb on + strand, within Psest_1881 at 1992.200 kb on - strand, within Psest_1881 at 1992.200 kb on - strand, within Psest_1881 at 1992.250 kb on - strand, within Psest_1881 at 1992.339 kb on + strand at 1992.339 kb on + strand at 1992.340 kb on - strand at 1992.340 kb on - strand at 1992.340 kb on - strand at 1992.340 kb on - strand at 1992.578 kb on + strand, within Psest_1882 at 1993.113 kb on + strand, within Psest_1882 at 1993.113 kb on + strand, within Psest_1882 at 1993.114 kb on - strand, within Psest_1882 at 1993.114 kb on - strand, within Psest_1882 at 1993.114 kb on - strand, within Psest_1882 at 1993.185 kb on - strand, within Psest_1882 at 1993.291 kb on - strand, within Psest_1882 at 1993.302 kb on + strand, within Psest_1882 at 1993.302 kb on + strand, within Psest_1882 at 1993.303 kb on - strand, within Psest_1882 at 1993.448 kb on + strand, within Psest_1882 at 1993.448 kb on + strand, within Psest_1882 at 1993.448 kb on + strand, within Psest_1882 at 1993.525 kb on - strand, within Psest_1882 at 1993.525 kb on - strand, within Psest_1882 at 1993.982 kb on - strand, within Psest_1883 at 1994.173 kb on - strand at 1994.195 kb on + strand at 1994.196 kb on - strand at 1994.196 kb on - strand at 1994.277 kb on + strand at 1994.278 kb on - strand at 1994.278 kb on - strand at 1994.342 kb on + strand at 1994.343 kb on - strand at 1994.348 kb on + strand at 1994.349 kb on - strand at 1994.682 kb on + strand, within Psest_1884 at 1994.683 kb on - strand, within Psest_1884
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,991,356 + Psest_1880 0.12 -0.6 1,991,357 - Psest_1880 0.12 +0.0 1,991,374 + Psest_1880 0.17 +1.3 1,991,374 + Psest_1880 0.17 -0.8 1,991,374 + Psest_1880 0.17 -1.5 1,991,374 + Psest_1880 0.17 +0.2 1,991,374 + Psest_1880 0.17 -1.2 1,991,375 - Psest_1880 0.17 -2.3 1,991,375 - Psest_1880 0.17 -1.4 1,991,504 + Psest_1880 0.52 -4.0 1,991,504 + Psest_1880 0.52 -0.5 1,991,504 + Psest_1880 0.52 -1.0 1,991,504 + Psest_1880 0.52 +1.9 1,991,504 + Psest_1880 0.52 -1.8 1,991,505 - Psest_1880 0.52 +1.6 1,991,505 - Psest_1880 0.52 -4.4 1,991,530 + Psest_1880 0.59 -0.4 1,991,530 + Psest_1880 0.59 -1.2 1,991,530 + Psest_1880 0.59 -1.5 1,991,530 + Psest_1880 0.59 -0.5 1,991,530 + Psest_1880 0.59 -3.1 1,991,531 - Psest_1880 0.59 +0.3 1,991,531 - Psest_1880 0.59 -1.9 1,991,531 - Psest_1880 0.59 -2.1 1,991,531 - Psest_1880 0.59 +0.9 1,991,531 - Psest_1880 0.59 -1.3 1,991,738 + -0.3 1,991,738 + -1.8 1,991,763 + -0.8 1,992,199 + Psest_1881 0.75 +0.9 1,992,199 + Psest_1881 0.75 +0.6 1,992,199 + Psest_1881 0.75 +0.4 1,992,199 + Psest_1881 0.75 -0.6 1,992,200 - Psest_1881 0.75 -2.2 1,992,200 - Psest_1881 0.75 +1.0 1,992,250 - Psest_1881 0.84 +1.0 1,992,339 + -2.1 1,992,339 + +0.2 1,992,340 - +0.4 1,992,340 - -0.9 1,992,340 - +0.1 1,992,340 - -0.8 1,992,578 + Psest_1882 0.18 -0.9 1,993,113 + Psest_1882 0.58 -1.6 1,993,113 + Psest_1882 0.58 -2.3 1,993,114 - Psest_1882 0.58 -0.8 1,993,114 - Psest_1882 0.58 -3.2 1,993,114 - Psest_1882 0.58 -2.1 1,993,185 - Psest_1882 0.63 -1.4 1,993,291 - Psest_1882 0.71 +0.2 1,993,302 + Psest_1882 0.72 +2.5 1,993,302 + Psest_1882 0.72 +0.3 1,993,303 - Psest_1882 0.72 -1.3 1,993,448 + Psest_1882 0.82 -0.3 1,993,448 + Psest_1882 0.82 +0.3 1,993,448 + Psest_1882 0.82 -1.5 1,993,525 - Psest_1882 0.88 -1.7 1,993,525 - Psest_1882 0.88 -1.1 1,993,982 - Psest_1883 0.50 +0.5 1,994,173 - +0.4 1,994,195 + +0.3 1,994,196 - -0.8 1,994,196 - -3.2 1,994,277 + -0.5 1,994,278 - -1.8 1,994,278 - -1.0 1,994,342 + +0.3 1,994,343 - +0.5 1,994,348 + -1.1 1,994,349 - -2.7 1,994,682 + Psest_1884 0.52 -0.4 1,994,683 - Psest_1884 0.52 -0.2
Or see this region's nucleotide sequence