Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1822

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1821 and Psest_1822 are separated by 9 nucleotidesPsest_1822 and Psest_1823 overlap by 4 nucleotidesPsest_1823 and Psest_1824 are separated by 19 nucleotides Psest_1821: Psest_1821 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 1,919,932 to 1,921,341 _1821 Psest_1822: Psest_1822 - Glycosyltransferases involved in cell wall biogenesis, at 1,921,351 to 1,922,103 _1822 Psest_1823: Psest_1823 - Nucleoside-diphosphate-sugar epimerases, at 1,922,100 to 1,923,041 _1823 Psest_1824: Psest_1824 - Sugar transferases involved in lipopolysaccharide synthesis, at 1,923,061 to 1,923,612 _1824 Position (kb) 1921 1922 1923Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5at 1920.352 kb on - strand, within Psest_1821at 1920.394 kb on + strand, within Psest_1821at 1920.394 kb on + strand, within Psest_1821at 1920.394 kb on + strand, within Psest_1821at 1920.396 kb on + strand, within Psest_1821at 1920.396 kb on + strand, within Psest_1821at 1920.397 kb on - strand, within Psest_1821at 1920.398 kb on + strand, within Psest_1821at 1920.398 kb on + strand, within Psest_1821at 1920.398 kb on + strand, within Psest_1821at 1920.398 kb on + strand, within Psest_1821at 1920.398 kb on + strand, within Psest_1821at 1920.399 kb on - strand, within Psest_1821at 1920.399 kb on - strand, within Psest_1821at 1920.399 kb on - strand, within Psest_1821at 1920.481 kb on + strand, within Psest_1821at 1920.481 kb on + strand, within Psest_1821at 1920.481 kb on + strand, within Psest_1821at 1920.481 kb on + strand, within Psest_1821at 1920.482 kb on - strand, within Psest_1821at 1920.482 kb on - strand, within Psest_1821at 1920.482 kb on - strand, within Psest_1821at 1920.499 kb on + strand, within Psest_1821at 1920.499 kb on + strand, within Psest_1821at 1920.499 kb on + strand, within Psest_1821at 1920.516 kb on + strand, within Psest_1821at 1920.516 kb on + strand, within Psest_1821at 1920.575 kb on + strand, within Psest_1821at 1920.619 kb on - strand, within Psest_1821at 1920.679 kb on + strand, within Psest_1821at 1920.680 kb on - strand, within Psest_1821at 1920.680 kb on - strand, within Psest_1821at 1920.774 kb on + strand, within Psest_1821at 1920.896 kb on + strand, within Psest_1821at 1921.030 kb on + strand, within Psest_1821at 1921.030 kb on + strand, within Psest_1821at 1921.030 kb on + strand, within Psest_1821at 1921.030 kb on + strand, within Psest_1821at 1921.031 kb on - strand, within Psest_1821at 1921.031 kb on - strand, within Psest_1821at 1921.031 kb on - strand, within Psest_1821at 1921.173 kb on + strand, within Psest_1821at 1921.180 kb on + strand, within Psest_1821at 1921.180 kb on + strand, within Psest_1821at 1921.180 kb on + strand, within Psest_1821at 1921.180 kb on + strand, within Psest_1821at 1921.181 kb on - strand, within Psest_1821at 1921.181 kb on - strand, within Psest_1821at 1921.207 kb on + strandat 1921.207 kb on + strandat 1921.208 kb on - strandat 1921.208 kb on - strandat 1921.208 kb on - strandat 1921.208 kb on - strandat 1921.208 kb on - strandat 1921.208 kb on - strandat 1921.208 kb on - strandat 1921.212 kb on - strandat 1921.250 kb on + strandat 1921.251 kb on - strandat 1921.369 kb on - strandat 1921.383 kb on + strandat 1921.384 kb on - strandat 1921.447 kb on + strand, within Psest_1822at 1921.447 kb on + strand, within Psest_1822at 1921.448 kb on - strand, within Psest_1822at 1921.448 kb on - strand, within Psest_1822at 1921.448 kb on - strand, within Psest_1822at 1921.448 kb on - strand, within Psest_1822at 1921.448 kb on - strand, within Psest_1822at 1921.493 kb on + strand, within Psest_1822at 1921.497 kb on + strand, within Psest_1822at 1921.497 kb on + strand, within Psest_1822at 1921.515 kb on + strand, within Psest_1822at 1921.516 kb on - strand, within Psest_1822at 1921.524 kb on + strand, within Psest_1822at 1921.685 kb on + strand, within Psest_1822at 1921.686 kb on - strand, within Psest_1822at 1921.686 kb on - strand, within Psest_1822at 1921.828 kb on + strand, within Psest_1822at 1921.845 kb on - strand, within Psest_1822at 1921.846 kb on + strand, within Psest_1822at 1921.846 kb on + strand, within Psest_1822at 1921.846 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.848 kb on + strand, within Psest_1822at 1921.849 kb on - strand, within Psest_1822at 1921.849 kb on - strand, within Psest_1822at 1921.849 kb on - strand, within Psest_1822at 1921.853 kb on + strand, within Psest_1822at 1921.853 kb on + strand, within Psest_1822at 1921.918 kb on + strand, within Psest_1822at 1922.080 kb on + strandat 1922.080 kb on + strandat 1922.080 kb on + strandat 1922.080 kb on + strandat 1922.093 kb on + strandat 1922.093 kb on + strandat 1922.094 kb on - strandat 1922.094 kb on - strandat 1922.094 kb on - strandat 1922.099 kb on + strandat 1922.099 kb on + strandat 1922.100 kb on - strandat 1922.134 kb on + strandat 1922.158 kb on + strandat 1922.158 kb on + strandat 1922.158 kb on + strandat 1922.158 kb on + strandat 1922.158 kb on + strandat 1922.159 kb on - strandat 1922.256 kb on - strand, within Psest_1823at 1922.290 kb on + strand, within Psest_1823at 1922.290 kb on + strand, within Psest_1823at 1922.318 kb on + strand, within Psest_1823at 1922.319 kb on - strand, within Psest_1823at 1922.385 kb on - strand, within Psest_1823at 1922.426 kb on + strand, within Psest_1823at 1922.426 kb on + strand, within Psest_1823at 1922.426 kb on + strand, within Psest_1823at 1922.427 kb on - strand, within Psest_1823at 1922.427 kb on - strand, within Psest_1823at 1922.427 kb on - strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.430 kb on + strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.431 kb on - strand, within Psest_1823at 1922.432 kb on + strand, within Psest_1823at 1922.433 kb on - strand, within Psest_1823at 1922.433 kb on - strand, within Psest_1823at 1922.623 kb on + strand, within Psest_1823at 1922.666 kb on + strand, within Psest_1823at 1922.666 kb on + strand, within Psest_1823at 1922.735 kb on + strand, within Psest_1823at 1922.735 kb on + strand, within Psest_1823at 1922.736 kb on - strand, within Psest_1823at 1922.736 kb on - strand, within Psest_1823at 1922.736 kb on - strand, within Psest_1823at 1922.776 kb on + strand, within Psest_1823at 1922.807 kb on + strand, within Psest_1823at 1922.808 kb on - strand, within Psest_1823at 1922.818 kb on + strand, within Psest_1823at 1922.818 kb on + strand, within Psest_1823at 1922.819 kb on - strand, within Psest_1823at 1922.821 kb on + strand, within Psest_1823at 1922.821 kb on + strand, within Psest_1823at 1922.846 kb on + strand, within Psest_1823at 1922.897 kb on + strand, within Psest_1823at 1922.897 kb on + strand, within Psest_1823at 1922.897 kb on + strand, within Psest_1823at 1922.898 kb on - strand, within Psest_1823at 1922.898 kb on - strand, within Psest_1823at 1922.898 kb on - strand, within Psest_1823at 1923.054 kb on - strandat 1923.070 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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1,920,352 - Psest_1821 0.30 +1.8
1,920,394 + Psest_1821 0.33 -1.6
1,920,394 + Psest_1821 0.33 +1.5
1,920,394 + Psest_1821 0.33 -1.4
1,920,396 + Psest_1821 0.33 +1.7
1,920,396 + Psest_1821 0.33 +4.8
1,920,397 - Psest_1821 0.33 +0.7
1,920,398 + Psest_1821 0.33 -0.5
1,920,398 + Psest_1821 0.33 -0.1
1,920,398 + Psest_1821 0.33 +3.6
1,920,398 + Psest_1821 0.33 +1.3
1,920,398 + Psest_1821 0.33 -0.0
1,920,399 - Psest_1821 0.33 -1.8
1,920,399 - Psest_1821 0.33 -0.2
1,920,399 - Psest_1821 0.33 +0.8
1,920,481 + Psest_1821 0.39 -1.1
1,920,481 + Psest_1821 0.39 -1.1
1,920,481 + Psest_1821 0.39 -2.7
1,920,481 + Psest_1821 0.39 +0.6
1,920,482 - Psest_1821 0.39 -4.8
1,920,482 - Psest_1821 0.39 -3.2
1,920,482 - Psest_1821 0.39 +1.4
1,920,499 + Psest_1821 0.40 +0.8
1,920,499 + Psest_1821 0.40 -2.0
1,920,499 + Psest_1821 0.40 +1.1
1,920,516 + Psest_1821 0.41 -3.2
1,920,516 + Psest_1821 0.41 +0.8
1,920,575 + Psest_1821 0.46 +0.0
1,920,619 - Psest_1821 0.49 +0.3
1,920,679 + Psest_1821 0.53 -3.6
1,920,680 - Psest_1821 0.53 +0.0
1,920,680 - Psest_1821 0.53 -2.7
1,920,774 + Psest_1821 0.60 -4.9
1,920,896 + Psest_1821 0.68 +0.6
1,921,030 + Psest_1821 0.78 -3.3
1,921,030 + Psest_1821 0.78 -0.8
1,921,030 + Psest_1821 0.78 +0.6
1,921,030 + Psest_1821 0.78 +0.2
1,921,031 - Psest_1821 0.78 -2.7
1,921,031 - Psest_1821 0.78 -4.2
1,921,031 - Psest_1821 0.78 -2.1
1,921,173 + Psest_1821 0.88 -1.1
1,921,180 + Psest_1821 0.89 +0.4
1,921,180 + Psest_1821 0.89 -2.6
1,921,180 + Psest_1821 0.89 +0.8
1,921,180 + Psest_1821 0.89 -1.5
1,921,181 - Psest_1821 0.89 -0.8
1,921,181 - Psest_1821 0.89 -2.3
1,921,207 + +1.8
1,921,207 + +0.4
1,921,208 - +2.1
1,921,208 - -0.3
1,921,208 - -3.9
1,921,208 - -0.6
1,921,208 - -1.6
1,921,208 - -2.6
1,921,208 - -2.9
1,921,212 - +0.2
1,921,250 + -4.1
1,921,251 - -2.8
1,921,369 - -0.5
1,921,383 + -2.3
1,921,384 - +2.4
1,921,447 + Psest_1822 0.13 -2.5
1,921,447 + Psest_1822 0.13 -4.0
1,921,448 - Psest_1822 0.13 +5.1
1,921,448 - Psest_1822 0.13 -2.7
1,921,448 - Psest_1822 0.13 -2.2
1,921,448 - Psest_1822 0.13 +1.3
1,921,448 - Psest_1822 0.13 -3.9
1,921,493 + Psest_1822 0.19 -2.9
1,921,497 + Psest_1822 0.19 -3.8
1,921,497 + Psest_1822 0.19 -1.2
1,921,515 + Psest_1822 0.22 -2.3
1,921,516 - Psest_1822 0.22 -1.2
1,921,524 + Psest_1822 0.23 -2.0
1,921,685 + Psest_1822 0.44 +0.6
1,921,686 - Psest_1822 0.44 -4.6
1,921,686 - Psest_1822 0.44 -0.4
1,921,828 + Psest_1822 0.63 +1.8
1,921,845 - Psest_1822 0.66 -1.4
1,921,846 + Psest_1822 0.66 -3.3
1,921,846 + Psest_1822 0.66 -3.8
1,921,846 + Psest_1822 0.66 -3.9
1,921,848 + Psest_1822 0.66 -0.9
1,921,848 + Psest_1822 0.66 +2.2
1,921,848 + Psest_1822 0.66 -2.2
1,921,848 + Psest_1822 0.66 -2.1
1,921,848 + Psest_1822 0.66 -0.4
1,921,848 + Psest_1822 0.66 -3.0
1,921,848 + Psest_1822 0.66 -1.1
1,921,848 + Psest_1822 0.66 -0.6
1,921,848 + Psest_1822 0.66 -3.1
1,921,848 + Psest_1822 0.66 -2.1
1,921,848 + Psest_1822 0.66 -2.5
1,921,849 - Psest_1822 0.66 -0.2
1,921,849 - Psest_1822 0.66 -1.4
1,921,849 - Psest_1822 0.66 -0.6
1,921,853 + Psest_1822 0.67 -3.5
1,921,853 + Psest_1822 0.67 -1.9
1,921,918 + Psest_1822 0.75 +0.9
1,922,080 + -0.9
1,922,080 + -3.1
1,922,080 + -1.4
1,922,080 + -4.3
1,922,093 + +2.2
1,922,093 + -3.0
1,922,094 - +1.6
1,922,094 - -2.4
1,922,094 - -3.5
1,922,099 + -0.4
1,922,099 + -0.9
1,922,100 - -1.1
1,922,134 + -0.3
1,922,158 + -3.4
1,922,158 + +0.4
1,922,158 + +1.2
1,922,158 + +0.1
1,922,158 + +1.4
1,922,159 - +0.5
1,922,256 - Psest_1823 0.17 +2.5
1,922,290 + Psest_1823 0.20 -3.1
1,922,290 + Psest_1823 0.20 -0.7
1,922,318 + Psest_1823 0.23 +1.7
1,922,319 - Psest_1823 0.23 -4.1
1,922,385 - Psest_1823 0.30 +0.4
1,922,426 + Psest_1823 0.35 +1.0
1,922,426 + Psest_1823 0.35 -1.0
1,922,426 + Psest_1823 0.35 -0.7
1,922,427 - Psest_1823 0.35 -2.9
1,922,427 - Psest_1823 0.35 -2.5
1,922,427 - Psest_1823 0.35 +2.5
1,922,430 + Psest_1823 0.35 -1.2
1,922,430 + Psest_1823 0.35 -2.7
1,922,430 + Psest_1823 0.35 -3.5
1,922,430 + Psest_1823 0.35 +0.6
1,922,430 + Psest_1823 0.35 -1.0
1,922,430 + Psest_1823 0.35 +1.2
1,922,430 + Psest_1823 0.35 +1.0
1,922,430 + Psest_1823 0.35 -0.1
1,922,430 + Psest_1823 0.35 +0.6
1,922,431 - Psest_1823 0.35 -2.0
1,922,431 - Psest_1823 0.35 +2.3
1,922,431 - Psest_1823 0.35 -1.6
1,922,431 - Psest_1823 0.35 +1.7
1,922,431 - Psest_1823 0.35 -3.3
1,922,431 - Psest_1823 0.35 +3.9
1,922,431 - Psest_1823 0.35 +1.4
1,922,431 - Psest_1823 0.35 +2.0
1,922,431 - Psest_1823 0.35 -3.8
1,922,431 - Psest_1823 0.35 -4.1
1,922,432 + Psest_1823 0.35 -2.1
1,922,433 - Psest_1823 0.35 -3.1
1,922,433 - Psest_1823 0.35 -0.3
1,922,623 + Psest_1823 0.56 -1.8
1,922,666 + Psest_1823 0.60 +2.7
1,922,666 + Psest_1823 0.60 +0.9
1,922,735 + Psest_1823 0.67 -3.3
1,922,735 + Psest_1823 0.67 -4.1
1,922,736 - Psest_1823 0.68 -3.9
1,922,736 - Psest_1823 0.68 -2.5
1,922,736 - Psest_1823 0.68 -2.4
1,922,776 + Psest_1823 0.72 -0.7
1,922,807 + Psest_1823 0.75 +1.9
1,922,808 - Psest_1823 0.75 +0.5
1,922,818 + Psest_1823 0.76 -2.0
1,922,818 + Psest_1823 0.76 -3.3
1,922,819 - Psest_1823 0.76 +0.7
1,922,821 + Psest_1823 0.77 -0.9
1,922,821 + Psest_1823 0.77 -3.3
1,922,846 + Psest_1823 0.79 +1.3
1,922,897 + Psest_1823 0.85 +0.1
1,922,897 + Psest_1823 0.85 -3.9
1,922,897 + Psest_1823 0.85 -0.5
1,922,898 - Psest_1823 0.85 -0.1
1,922,898 - Psest_1823 0.85 -0.8
1,922,898 - Psest_1823 0.85 +1.9
1,923,054 - -2.0
1,923,070 - +1.9

Or see this region's nucleotide sequence