Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0172 and Psest_0173 overlap by 4 nucleotides Psest_0173 and Psest_0174 are separated by 129 nucleotides Psest_0174 and Psest_0175 are separated by 16 nucleotides Psest_0175 and Psest_0176 are separated by 1 nucleotides
Psest_0172: Psest_0172 - Uncharacterized protein conserved in bacteria, at 166,834 to 167,148
_0172
Psest_0173: Psest_0173 - TIGR02449 family protein, at 167,145 to 167,354
_0173
Psest_0174: Psest_0174 - Uncharacterized protein conserved in bacteria, at 167,484 to 168,038
_0174
Psest_0175: Psest_0175 - Xaa-Pro aminopeptidase, at 168,055 to 169,389
_0175
Psest_0176: Psest_0176 - 2-polyprenyl-6-methoxyphenol 4-hydroxylase, at 169,391 to 170,569
_0176
Position (kb) 168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.103 kb on - strand at 167.103 kb on - strand, within Psest_0172 at 167.103 kb on - strand, within Psest_0172 at 167.433 kb on - strand at 167.433 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.940 kb on + strand, within Psest_0174 at 167.940 kb on + strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.958 kb on + strand, within Psest_0174 at 167.958 kb on + strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.154 kb on + strand at 168.274 kb on + strand, within Psest_0175 at 168.348 kb on + strand, within Psest_0175 at 168.436 kb on + strand, within Psest_0175 at 168.437 kb on - strand, within Psest_0175 at 168.440 kb on - strand, within Psest_0175 at 168.784 kb on + strand, within Psest_0175 at 168.856 kb on + strand, within Psest_0175 at 168.877 kb on - strand, within Psest_0175 at 168.933 kb on - strand, within Psest_0175 at 168.963 kb on + strand, within Psest_0175 at 169.084 kb on + strand, within Psest_0175 at 169.096 kb on + strand, within Psest_0175 at 169.096 kb on + strand, within Psest_0175 at 169.096 kb on + strand, within Psest_0175 at 169.096 kb on + strand, within Psest_0175 at 169.096 kb on + strand, within Psest_0175 at 169.096 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.216 kb on + strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.217 kb on - strand, within Psest_0175 at 169.218 kb on + strand, within Psest_0175
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 167,102 + Psest_0172 0.85 +1.3 167,102 + Psest_0172 0.85 +1.6 167,102 + Psest_0172 0.85 +0.3 167,103 - +1.3 167,103 - Psest_0172 0.85 +1.5 167,103 - Psest_0172 0.85 -0.0 167,433 - -2.3 167,433 - -0.5 167,454 - -2.7 167,454 - -2.7 167,454 - -0.5 167,454 - -3.4 167,940 + Psest_0174 0.82 +0.5 167,940 + Psest_0174 0.82 -0.6 167,941 - Psest_0174 0.82 -0.1 167,941 - Psest_0174 0.82 -0.7 167,941 - Psest_0174 0.82 -0.9 167,958 + Psest_0174 0.85 -2.7 167,958 + Psest_0174 0.85 -3.3 167,959 - Psest_0174 0.86 -2.2 167,959 - Psest_0174 0.86 +0.9 167,959 - Psest_0174 0.86 -3.3 167,959 - Psest_0174 0.86 -0.2 168,154 + +1.7 168,154 + +0.9 168,154 + +1.3 168,154 + -0.3 168,154 + -0.3 168,154 + -1.9 168,154 + +0.6 168,274 + Psest_0175 0.16 -0.0 168,348 + Psest_0175 0.22 -0.7 168,436 + Psest_0175 0.29 -2.0 168,437 - Psest_0175 0.29 -0.5 168,440 - Psest_0175 0.29 -1.3 168,784 + Psest_0175 0.55 +0.6 168,856 + Psest_0175 0.60 +1.9 168,877 - Psest_0175 0.62 -1.5 168,933 - Psest_0175 0.66 -0.5 168,963 + Psest_0175 0.68 -2.9 169,084 + Psest_0175 0.77 +0.7 169,096 + Psest_0175 0.78 -0.7 169,096 + Psest_0175 0.78 +1.3 169,096 + Psest_0175 0.78 +0.7 169,096 + Psest_0175 0.78 +1.6 169,096 + Psest_0175 0.78 -2.1 169,096 + Psest_0175 0.78 -2.9 169,216 + Psest_0175 0.87 +0.2 169,216 + Psest_0175 0.87 +1.0 169,216 + Psest_0175 0.87 -0.4 169,216 + Psest_0175 0.87 -1.0 169,216 + Psest_0175 0.87 -2.0 169,216 + Psest_0175 0.87 -1.7 169,216 + Psest_0175 0.87 -2.5 169,216 + Psest_0175 0.87 -0.5 169,216 + Psest_0175 0.87 +0.7 169,217 - Psest_0175 0.87 -0.9 169,217 - Psest_0175 0.87 +1.3 169,217 - Psest_0175 0.87 -1.5 169,217 - Psest_0175 0.87 -1.0 169,217 - Psest_0175 0.87 -2.8 169,217 - Psest_0175 0.87 -1.7 169,217 - Psest_0175 0.87 -3.1 169,217 - Psest_0175 0.87 -2.0 169,217 - Psest_0175 0.87 -2.1 169,217 - Psest_0175 0.87 -1.0 169,217 - Psest_0175 0.87 -1.5 169,217 - Psest_0175 0.87 +0.6 169,217 - Psest_0175 0.87 -2.0 169,217 - Psest_0175 0.87 -1.7 169,217 - Psest_0175 0.87 -0.7 169,217 - Psest_0175 0.87 -1.9 169,217 - Psest_0175 0.87 +0.3 169,218 + Psest_0175 0.87 +0.1
Or see this region's nucleotide sequence