Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0154 and Psest_0155 are separated by 36 nucleotides Psest_0155 and Psest_0156 are separated by 98 nucleotides Psest_0156 and Psest_0157 are separated by 86 nucleotides
Psest_0154: Psest_0154 - Uncharacterized conserved protein, at 153,331 to 153,591
_0154
Psest_0155: Psest_0155 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 153,628 to 154,371
_0155
Psest_0156: Psest_0156 - imidazoleglycerol phosphate synthase, cyclase subunit, at 154,470 to 155,240
_0156
Psest_0157: Psest_0157 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 155,327 to 156,076
_0157
Position (kb) 154
155
156 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 153.499 kb on + strand, within Psest_0154 at 153.499 kb on + strand, within Psest_0154 at 153.499 kb on + strand, within Psest_0154 at 153.500 kb on - strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.904 kb on + strand, within Psest_0155 at 153.904 kb on + strand, within Psest_0155 at 153.904 kb on + strand, within Psest_0155 at 153.905 kb on - strand, within Psest_0155 at 153.905 kb on - strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.926 kb on - strand, within Psest_0155 at 153.926 kb on - strand, within Psest_0155 at 153.926 kb on - strand, within Psest_0155 at 154.123 kb on + strand, within Psest_0155 at 154.306 kb on + strand at 154.306 kb on + strand at 154.306 kb on + strand at 154.307 kb on - strand at 154.307 kb on - strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.358 kb on - strand at 154.412 kb on + strand at 154.412 kb on + strand at 154.412 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.670 kb on - strand, within Psest_0156 at 155.184 kb on + strand at 155.184 kb on + strand at 155.184 kb on + strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.208 kb on + strand at 155.209 kb on - strand at 155.266 kb on + strand at 155.267 kb on - strand at 155.267 kb on - strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.368 kb on - strand at 155.438 kb on + strand, within Psest_0157 at 155.439 kb on - strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.577 kb on - strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.951 kb on - strand, within Psest_0157 at 155.963 kb on + strand, within Psest_0157 at 155.964 kb on - strand, within Psest_0157 at 156.069 kb on - strand at 156.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 153,499 + Psest_0154 0.64 -1.7 153,499 + Psest_0154 0.64 +0.9 153,499 + Psest_0154 0.64 -1.2 153,500 - Psest_0154 0.65 +0.9 153,502 + Psest_0154 0.66 -0.5 153,502 + Psest_0154 0.66 -1.3 153,502 + Psest_0154 0.66 +3.1 153,502 + Psest_0154 0.66 -0.2 153,502 + Psest_0154 0.66 -0.2 153,502 + Psest_0154 0.66 +0.2 153,502 + Psest_0154 0.66 +2.0 153,502 + Psest_0154 0.66 -1.7 153,502 + Psest_0154 0.66 -1.5 153,502 + Psest_0154 0.66 -1.5 153,503 - Psest_0154 0.66 +1.9 153,503 - Psest_0154 0.66 -1.7 153,503 - Psest_0154 0.66 -2.4 153,503 - Psest_0154 0.66 -2.9 153,503 - Psest_0154 0.66 -2.3 153,503 - Psest_0154 0.66 -0.9 153,904 + Psest_0155 0.37 -0.4 153,904 + Psest_0155 0.37 -2.9 153,904 + Psest_0155 0.37 +0.6 153,905 - Psest_0155 0.37 +0.8 153,905 - Psest_0155 0.37 +0.6 153,925 + Psest_0155 0.40 -0.2 153,925 + Psest_0155 0.40 -0.9 153,925 + Psest_0155 0.40 -3.7 153,925 + Psest_0155 0.40 -1.2 153,925 + Psest_0155 0.40 +1.3 153,925 + Psest_0155 0.40 -0.9 153,926 - Psest_0155 0.40 -3.5 153,926 - Psest_0155 0.40 +2.3 153,926 - Psest_0155 0.40 -3.7 154,123 + Psest_0155 0.67 +1.3 154,306 + +0.7 154,306 + -0.9 154,306 + +0.5 154,307 - -2.8 154,307 - +0.7 154,357 + +0.0 154,357 + -0.6 154,357 + -1.3 154,357 + -2.9 154,357 + -1.5 154,358 - -1.2 154,412 + +0.3 154,412 + -0.3 154,412 + -0.4 154,419 + +1.1 154,419 + -0.7 154,419 + +0.5 154,419 + +0.4 154,419 + -3.1 154,420 - -2.5 154,420 - +0.8 154,420 - +1.8 154,420 - -3.0 154,420 - -1.3 154,670 - Psest_0156 0.26 +3.0 155,184 + -0.5 155,184 + -1.0 155,184 + -3.9 155,185 - +0.3 155,185 - -0.9 155,185 - +0.5 155,185 - -0.7 155,208 + -3.1 155,209 - -0.6 155,266 + +0.0 155,267 - -0.2 155,267 - -3.3 155,277 + -0.4 155,277 + -1.9 155,277 + +0.6 155,277 + +0.3 155,277 + -1.9 155,278 - -0.9 155,278 - +1.3 155,278 - -1.1 155,278 - +0.1 155,278 - +1.7 155,278 - -2.7 155,368 - -3.4 155,438 + Psest_0157 0.15 -2.4 155,439 - Psest_0157 0.15 +0.4 155,540 + Psest_0157 0.28 +1.1 155,540 + Psest_0157 0.28 +1.4 155,576 + Psest_0157 0.33 -3.1 155,576 + Psest_0157 0.33 +1.5 155,576 + Psest_0157 0.33 -2.2 155,577 - Psest_0157 0.33 +0.4 155,603 + Psest_0157 0.37 +0.7 155,603 + Psest_0157 0.37 +4.1 155,603 + Psest_0157 0.37 +0.3 155,603 + Psest_0157 0.37 -0.1 155,604 - Psest_0157 0.37 -0.2 155,604 - Psest_0157 0.37 +1.8 155,744 + Psest_0157 0.56 -2.2 155,744 + Psest_0157 0.56 -2.9 155,744 + Psest_0157 0.56 -1.5 155,744 + Psest_0157 0.56 -0.4 155,744 + Psest_0157 0.56 -3.9 155,744 + Psest_0157 0.56 -2.0 155,745 - Psest_0157 0.56 -1.8 155,745 - Psest_0157 0.56 +0.1 155,745 - Psest_0157 0.56 -2.5 155,759 + Psest_0157 0.58 -1.3 155,759 + Psest_0157 0.58 -2.8 155,760 - Psest_0157 0.58 +0.7 155,760 - Psest_0157 0.58 +0.6 155,760 - Psest_0157 0.58 +0.3 155,760 - Psest_0157 0.58 +2.5 155,951 - Psest_0157 0.83 +0.2 155,963 + Psest_0157 0.85 +0.7 155,964 - Psest_0157 0.85 -0.9 156,069 - +2.2 156,069 - +1.9
Or see this region's nucleotide sequence