Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0150 and Psest_0151 are separated by 357 nucleotides Psest_0151 and Psest_0152 are separated by 129 nucleotides Psest_0152 and Psest_0153 overlap by 1 nucleotides
Psest_0150: Psest_0150 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 148,970 to 150,301
_0150
Psest_0151: Psest_0151 - Response regulator with putative antiterminator output domain, at 150,659 to 151,969
_0151
Psest_0152: Psest_0152 - Imidazoleglycerol-phosphate dehydratase, at 152,099 to 152,692
_0152
Psest_0153: Psest_0153 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 152,692 to 153,330
_0153
Position (kb) 150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 149.829 kb on + strand, within Psest_0150 at 149.935 kb on - strand, within Psest_0150 at 150.032 kb on + strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.725 kb on + strand at 150.725 kb on + strand at 150.726 kb on - strand at 150.726 kb on - strand at 150.789 kb on + strand at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 151.299 kb on - strand, within Psest_0151 at 151.654 kb on - strand, within Psest_0151 at 151.654 kb on - strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.810 kb on - strand, within Psest_0151 at 151.862 kb on - strand at 152.058 kb on + strand at 152.058 kb on + strand at 152.059 kb on - strand at 152.059 kb on - strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.061 kb on - strand at 152.061 kb on - strand at 152.111 kb on - strand at 152.111 kb on - strand at 152.111 kb on - strand at 152.117 kb on + strand at 152.117 kb on + strand at 152.118 kb on - strand at 152.118 kb on - strand at 152.118 kb on - strand at 152.121 kb on + strand at 152.122 kb on - strand at 152.122 kb on - strand at 152.122 kb on - strand at 152.339 kb on - strand, within Psest_0152 at 152.339 kb on - strand, within Psest_0152 at 152.387 kb on + strand, within Psest_0152 at 152.387 kb on + strand, within Psest_0152 at 152.388 kb on - strand, within Psest_0152 at 152.388 kb on - strand, within Psest_0152 at 152.465 kb on + strand, within Psest_0152 at 152.466 kb on - strand, within Psest_0152 at 152.466 kb on - strand, within Psest_0152 at 152.555 kb on - strand, within Psest_0152 at 152.690 kb on + strand at 152.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 149,829 + Psest_0150 0.64 -2.1 149,935 - Psest_0150 0.72 -2.5 150,032 + Psest_0150 0.80 -3.2 150,033 - Psest_0150 0.80 -0.5 150,033 - Psest_0150 0.80 -3.2 150,033 - Psest_0150 0.80 -1.1 150,033 - Psest_0150 0.80 -2.5 150,054 + Psest_0150 0.81 +0.6 150,054 + Psest_0150 0.81 -2.8 150,055 - Psest_0150 0.81 -1.2 150,055 - Psest_0150 0.81 +0.6 150,725 + -2.2 150,725 + -0.4 150,726 - -3.5 150,726 - +0.1 150,789 + -2.3 150,790 - Psest_0151 0.10 -1.2 150,790 - Psest_0151 0.10 +1.3 150,790 - Psest_0151 0.10 +0.5 150,912 - Psest_0151 0.19 -0.6 150,912 - Psest_0151 0.19 -1.7 151,299 - Psest_0151 0.49 +1.4 151,654 - Psest_0151 0.76 +0.0 151,654 - Psest_0151 0.76 -1.1 151,809 + Psest_0151 0.88 -0.8 151,809 + Psest_0151 0.88 -3.4 151,809 + Psest_0151 0.88 -1.9 151,809 + Psest_0151 0.88 -1.0 151,809 + Psest_0151 0.88 +0.2 151,809 + Psest_0151 0.88 -2.0 151,810 - Psest_0151 0.88 -1.9 151,862 - -0.2 152,058 + -1.0 152,058 + -3.1 152,059 - -0.4 152,059 - +1.4 152,060 + -1.5 152,060 + +0.6 152,060 + -1.0 152,060 + -1.2 152,060 + -1.5 152,061 - -0.7 152,061 - -1.8 152,111 - +1.0 152,111 - +0.3 152,111 - -1.4 152,117 + -2.0 152,117 + +1.4 152,118 - -1.5 152,118 - -2.0 152,118 - -0.1 152,121 + -0.2 152,122 - -0.3 152,122 - +0.4 152,122 - -3.1 152,339 - Psest_0152 0.40 -0.6 152,339 - Psest_0152 0.40 -1.1 152,387 + Psest_0152 0.48 -0.7 152,387 + Psest_0152 0.48 -2.2 152,388 - Psest_0152 0.49 -3.7 152,388 - Psest_0152 0.49 -1.4 152,465 + Psest_0152 0.62 -0.8 152,466 - Psest_0152 0.62 -0.9 152,466 - Psest_0152 0.62 -1.8 152,555 - Psest_0152 0.77 -2.2 152,690 + -2.8 152,691 - -3.0
Or see this region's nucleotide sequence