Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0148 and Psest_0149 are separated by 11 nucleotides Psest_0149 and Psest_0150 are separated by 26 nucleotides Psest_0150 and Psest_0151 are separated by 357 nucleotides
Psest_0148: Psest_0148 - nitrate transport ATP-binding subunits C and D, at 147,280 to 148,062
_0148
Psest_0149: Psest_0149 - nitrate ABC transporter, permease protein, at 148,074 to 148,943
_0149
Psest_0150: Psest_0150 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 148,970 to 150,301
_0150
Psest_0151: Psest_0151 - Response regulator with putative antiterminator output domain, at 150,659 to 151,969
_0151
Position (kb) 148
149
150
151 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.157 kb on - strand at 148.291 kb on - strand, within Psest_0149 at 148.306 kb on + strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.342 kb on + strand, within Psest_0149 at 148.627 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.700 kb on - strand, within Psest_0149 at 148.700 kb on - strand, within Psest_0149 at 149.004 kb on + strand at 149.005 kb on - strand at 149.235 kb on + strand, within Psest_0150 at 149.235 kb on + strand, within Psest_0150 at 149.256 kb on + strand, within Psest_0150 at 149.340 kb on + strand, within Psest_0150 at 149.340 kb on + strand, within Psest_0150 at 149.341 kb on - strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.476 kb on - strand, within Psest_0150 at 149.544 kb on + strand, within Psest_0150 at 149.829 kb on + strand, within Psest_0150 at 149.935 kb on - strand, within Psest_0150 at 150.032 kb on + strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.725 kb on + strand at 150.725 kb on + strand at 150.726 kb on - strand at 150.726 kb on - strand at 150.789 kb on + strand at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 151.299 kb on - strand, within Psest_0151
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 148,055 + +2.0 148,055 + -1.9 148,055 + -0.5 148,055 + -0.9 148,055 + +0.1 148,055 + -0.4 148,055 + -1.3 148,055 + -2.1 148,056 - -3.7 148,056 - -1.9 148,056 - -0.2 148,056 - -2.7 148,056 - -2.1 148,056 - +0.8 148,056 - +1.0 148,056 - -2.2 148,056 - -3.3 148,156 + -1.0 148,156 + -3.1 148,156 + -1.4 148,156 + +0.0 148,157 - -1.3 148,291 - Psest_0149 0.25 -0.8 148,306 + Psest_0149 0.27 +0.8 148,307 - Psest_0149 0.27 +1.9 148,307 - Psest_0149 0.27 +1.3 148,307 - Psest_0149 0.27 -2.3 148,307 - Psest_0149 0.27 -0.3 148,307 - Psest_0149 0.27 -0.7 148,307 - Psest_0149 0.27 -2.5 148,307 - Psest_0149 0.27 +0.9 148,307 - Psest_0149 0.27 +1.3 148,307 - Psest_0149 0.27 -1.0 148,342 + Psest_0149 0.31 -2.4 148,627 + Psest_0149 0.64 -3.2 148,699 + Psest_0149 0.72 -3.9 148,699 + Psest_0149 0.72 -2.6 148,699 + Psest_0149 0.72 +0.0 148,700 - Psest_0149 0.72 -2.3 148,700 - Psest_0149 0.72 +0.1 149,004 + -2.1 149,005 - -1.5 149,235 + Psest_0150 0.20 -3.3 149,235 + Psest_0150 0.20 -1.4 149,256 + Psest_0150 0.21 -1.3 149,340 + Psest_0150 0.28 -0.1 149,340 + Psest_0150 0.28 -2.0 149,341 - Psest_0150 0.28 -2.3 149,475 + Psest_0150 0.38 +0.6 149,475 + Psest_0150 0.38 +0.4 149,475 + Psest_0150 0.38 -3.3 149,475 + Psest_0150 0.38 -2.8 149,476 - Psest_0150 0.38 -0.4 149,544 + Psest_0150 0.43 -0.2 149,829 + Psest_0150 0.64 -2.1 149,935 - Psest_0150 0.72 -2.5 150,032 + Psest_0150 0.80 -3.2 150,033 - Psest_0150 0.80 -0.5 150,033 - Psest_0150 0.80 -3.2 150,033 - Psest_0150 0.80 -1.1 150,033 - Psest_0150 0.80 -2.5 150,054 + Psest_0150 0.81 +0.6 150,054 + Psest_0150 0.81 -2.8 150,055 - Psest_0150 0.81 -1.2 150,055 - Psest_0150 0.81 +0.6 150,725 + -2.2 150,725 + -0.4 150,726 - -3.5 150,726 - +0.1 150,789 + -2.3 150,790 - Psest_0151 0.10 -1.2 150,790 - Psest_0151 0.10 +1.3 150,790 - Psest_0151 0.10 +0.5 150,912 - Psest_0151 0.19 -0.6 150,912 - Psest_0151 0.19 -1.7 151,299 - Psest_0151 0.49 +1.4
Or see this region's nucleotide sequence