Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1485 and Psest_1486 are separated by 5 nucleotides Psest_1486 and Psest_1487 are separated by 9 nucleotides Psest_1487 and Psest_1488 are separated by 160 nucleotides
Psest_1485: Psest_1485 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 1,587,268 to 1,587,840
_1485
Psest_1486: Psest_1486 - hypothetical protein, at 1,587,846 to 1,588,778
_1486
Psest_1487: Psest_1487 - prolyl-tRNA synthetase, family II, at 1,588,788 to 1,590,503
_1487
Psest_1488: Psest_1488 - outer membrane porin, OprD family., at 1,590,664 to 1,591,920
_1488
Position (kb)
1588
1589
1590
1591 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1588.280 kb on - strand, within Psest_1486 at 1588.280 kb on - strand, within Psest_1486 at 1588.456 kb on - strand, within Psest_1486 at 1588.615 kb on - strand, within Psest_1486 at 1588.647 kb on - strand, within Psest_1486 at 1590.550 kb on - strand at 1590.550 kb on - strand at 1590.722 kb on + strand at 1590.722 kb on + strand at 1590.722 kb on + strand at 1590.722 kb on + strand at 1590.722 kb on + strand at 1590.723 kb on - strand at 1590.723 kb on - strand at 1590.723 kb on - strand at 1590.753 kb on - strand at 1590.753 kb on - strand at 1590.753 kb on - strand at 1590.753 kb on - strand at 1590.762 kb on + strand at 1590.762 kb on + strand at 1590.803 kb on - strand, within Psest_1488 at 1590.810 kb on - strand, within Psest_1488 at 1590.841 kb on - strand, within Psest_1488 at 1590.881 kb on + strand, within Psest_1488 at 1590.881 kb on + strand, within Psest_1488 at 1590.882 kb on - strand, within Psest_1488 at 1590.935 kb on + strand, within Psest_1488 at 1590.935 kb on + strand, within Psest_1488 at 1590.981 kb on - strand, within Psest_1488 at 1590.981 kb on - strand, within Psest_1488 at 1590.983 kb on + strand, within Psest_1488 at 1590.983 kb on + strand, within Psest_1488 at 1590.983 kb on + strand, within Psest_1488 at 1590.984 kb on - strand, within Psest_1488 at 1590.984 kb on - strand, within Psest_1488 at 1590.984 kb on - strand, within Psest_1488 at 1590.984 kb on - strand, within Psest_1488 at 1591.029 kb on - strand, within Psest_1488 at 1591.034 kb on + strand, within Psest_1488 at 1591.034 kb on + strand, within Psest_1488 at 1591.035 kb on - strand, within Psest_1488 at 1591.035 kb on - strand, within Psest_1488 at 1591.035 kb on - strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.094 kb on + strand, within Psest_1488 at 1591.095 kb on - strand, within Psest_1488 at 1591.095 kb on - strand, within Psest_1488 at 1591.095 kb on - strand, within Psest_1488 at 1591.095 kb on - strand, within Psest_1488 at 1591.095 kb on - strand, within Psest_1488 at 1591.095 kb on - strand, within Psest_1488 at 1591.154 kb on + strand, within Psest_1488 at 1591.155 kb on - strand, within Psest_1488 at 1591.158 kb on + strand, within Psest_1488 at 1591.158 kb on + strand, within Psest_1488 at 1591.159 kb on - strand, within Psest_1488 at 1591.159 kb on - strand, within Psest_1488 at 1591.159 kb on - strand, within Psest_1488 at 1591.167 kb on - strand, within Psest_1488 at 1591.217 kb on + strand, within Psest_1488 at 1591.217 kb on + strand, within Psest_1488 at 1591.217 kb on + strand, within Psest_1488 at 1591.217 kb on + strand, within Psest_1488 at 1591.217 kb on + strand, within Psest_1488 at 1591.218 kb on - strand, within Psest_1488 at 1591.218 kb on - strand, within Psest_1488 at 1591.218 kb on - strand, within Psest_1488 at 1591.232 kb on + strand, within Psest_1488 at 1591.232 kb on + strand, within Psest_1488 at 1591.233 kb on - strand, within Psest_1488 at 1591.265 kb on + strand, within Psest_1488 at 1591.268 kb on + strand, within Psest_1488 at 1591.268 kb on + strand, within Psest_1488 at 1591.268 kb on + strand, within Psest_1488 at 1591.268 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.301 kb on + strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.302 kb on - strand, within Psest_1488 at 1591.312 kb on + strand, within Psest_1488 at 1591.312 kb on + strand, within Psest_1488 at 1591.312 kb on + strand, within Psest_1488 at 1591.312 kb on + strand, within Psest_1488 at 1591.312 kb on + strand, within Psest_1488 at 1591.312 kb on + strand, within Psest_1488 at 1591.312 kb on + strand, within Psest_1488 at 1591.313 kb on - strand, within Psest_1488 at 1591.313 kb on - strand, within Psest_1488 at 1591.313 kb on - strand, within Psest_1488 at 1591.313 kb on - strand, within Psest_1488 at 1591.342 kb on + strand, within Psest_1488 at 1591.342 kb on + strand, within Psest_1488 at 1591.343 kb on - strand, within Psest_1488 at 1591.343 kb on - strand, within Psest_1488 at 1591.343 kb on - strand, within Psest_1488 at 1591.343 kb on - strand, within Psest_1488 at 1591.346 kb on + strand, within Psest_1488 at 1591.346 kb on + strand, within Psest_1488 at 1591.346 kb on + strand, within Psest_1488 at 1591.346 kb on + strand, within Psest_1488 at 1591.346 kb on + strand, within Psest_1488 at 1591.347 kb on - strand, within Psest_1488 at 1591.347 kb on - strand, within Psest_1488 at 1591.350 kb on + strand, within Psest_1488 at 1591.350 kb on + strand, within Psest_1488 at 1591.350 kb on + strand, within Psest_1488 at 1591.350 kb on + strand, within Psest_1488 at 1591.484 kb on + strand, within Psest_1488 at 1591.484 kb on + strand, within Psest_1488 at 1591.484 kb on + strand, within Psest_1488 at 1591.484 kb on + strand, within Psest_1488 at 1591.485 kb on - strand, within Psest_1488 at 1591.485 kb on - strand, within Psest_1488 at 1591.485 kb on - strand, within Psest_1488
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,588,280 - Psest_1486 0.47 -2.1 1,588,280 - Psest_1486 0.47 -1.8 1,588,456 - Psest_1486 0.65 -0.1 1,588,615 - Psest_1486 0.82 -4.3 1,588,647 - Psest_1486 0.86 -2.8 1,590,550 - -2.2 1,590,550 - -0.6 1,590,722 + -2.8 1,590,722 + -4.2 1,590,722 + +0.0 1,590,722 + -2.5 1,590,722 + +1.7 1,590,723 - -1.0 1,590,723 - -0.2 1,590,723 - +1.1 1,590,753 - +0.9 1,590,753 - -3.0 1,590,753 - +0.1 1,590,753 - -0.1 1,590,762 + +0.5 1,590,762 + +0.7 1,590,803 - Psest_1488 0.11 -2.7 1,590,810 - Psest_1488 0.12 -0.5 1,590,841 - Psest_1488 0.14 -0.3 1,590,881 + Psest_1488 0.17 -2.3 1,590,881 + Psest_1488 0.17 +0.4 1,590,882 - Psest_1488 0.17 -0.7 1,590,935 + Psest_1488 0.22 -1.5 1,590,935 + Psest_1488 0.22 +1.9 1,590,981 - Psest_1488 0.25 -2.6 1,590,981 - Psest_1488 0.25 -2.5 1,590,983 + Psest_1488 0.25 +0.0 1,590,983 + Psest_1488 0.25 -2.5 1,590,983 + Psest_1488 0.25 +2.7 1,590,984 - Psest_1488 0.25 -2.0 1,590,984 - Psest_1488 0.25 -0.3 1,590,984 - Psest_1488 0.25 +1.0 1,590,984 - Psest_1488 0.25 -1.4 1,591,029 - Psest_1488 0.29 -2.5 1,591,034 + Psest_1488 0.29 -3.5 1,591,034 + Psest_1488 0.29 -2.3 1,591,035 - Psest_1488 0.30 +1.2 1,591,035 - Psest_1488 0.30 -3.1 1,591,035 - Psest_1488 0.30 -0.3 1,591,094 + Psest_1488 0.34 +0.3 1,591,094 + Psest_1488 0.34 -3.4 1,591,094 + Psest_1488 0.34 +0.7 1,591,094 + Psest_1488 0.34 +0.6 1,591,094 + Psest_1488 0.34 -0.2 1,591,094 + Psest_1488 0.34 -2.1 1,591,094 + Psest_1488 0.34 -3.2 1,591,094 + Psest_1488 0.34 -3.0 1,591,094 + Psest_1488 0.34 -0.7 1,591,095 - Psest_1488 0.34 +0.2 1,591,095 - Psest_1488 0.34 -2.5 1,591,095 - Psest_1488 0.34 -1.5 1,591,095 - Psest_1488 0.34 +1.0 1,591,095 - Psest_1488 0.34 -1.5 1,591,095 - Psest_1488 0.34 -1.7 1,591,154 + Psest_1488 0.39 -1.2 1,591,155 - Psest_1488 0.39 +1.2 1,591,158 + Psest_1488 0.39 +0.2 1,591,158 + Psest_1488 0.39 -1.8 1,591,159 - Psest_1488 0.39 +0.5 1,591,159 - Psest_1488 0.39 -0.7 1,591,159 - Psest_1488 0.39 +1.3 1,591,167 - Psest_1488 0.40 -0.3 1,591,217 + Psest_1488 0.44 -3.7 1,591,217 + Psest_1488 0.44 -2.0 1,591,217 + Psest_1488 0.44 -2.4 1,591,217 + Psest_1488 0.44 -2.4 1,591,217 + Psest_1488 0.44 +1.1 1,591,218 - Psest_1488 0.44 -4.3 1,591,218 - Psest_1488 0.44 +1.5 1,591,218 - Psest_1488 0.44 +0.3 1,591,232 + Psest_1488 0.45 -1.7 1,591,232 + Psest_1488 0.45 -3.5 1,591,233 - Psest_1488 0.45 -1.4 1,591,265 + Psest_1488 0.48 -0.7 1,591,268 + Psest_1488 0.48 -2.8 1,591,268 + Psest_1488 0.48 +0.4 1,591,268 + Psest_1488 0.48 +0.8 1,591,268 + Psest_1488 0.48 +1.5 1,591,301 + Psest_1488 0.51 -3.1 1,591,301 + Psest_1488 0.51 -2.3 1,591,301 + Psest_1488 0.51 +1.1 1,591,301 + Psest_1488 0.51 -3.3 1,591,301 + Psest_1488 0.51 +0.0 1,591,301 + Psest_1488 0.51 -2.1 1,591,301 + Psest_1488 0.51 -1.0 1,591,301 + Psest_1488 0.51 -3.2 1,591,301 + Psest_1488 0.51 -2.7 1,591,302 - Psest_1488 0.51 -3.3 1,591,302 - Psest_1488 0.51 -1.7 1,591,302 - Psest_1488 0.51 +1.7 1,591,302 - Psest_1488 0.51 +1.5 1,591,302 - Psest_1488 0.51 -3.5 1,591,302 - Psest_1488 0.51 -3.0 1,591,302 - Psest_1488 0.51 -1.5 1,591,302 - Psest_1488 0.51 -0.5 1,591,312 + Psest_1488 0.52 -3.1 1,591,312 + Psest_1488 0.52 +1.6 1,591,312 + Psest_1488 0.52 -3.2 1,591,312 + Psest_1488 0.52 -0.8 1,591,312 + Psest_1488 0.52 +0.6 1,591,312 + Psest_1488 0.52 -0.7 1,591,312 + Psest_1488 0.52 +1.3 1,591,313 - Psest_1488 0.52 -3.5 1,591,313 - Psest_1488 0.52 -4.5 1,591,313 - Psest_1488 0.52 +0.0 1,591,313 - Psest_1488 0.52 -1.0 1,591,342 + Psest_1488 0.54 +1.3 1,591,342 + Psest_1488 0.54 -3.8 1,591,343 - Psest_1488 0.54 -1.3 1,591,343 - Psest_1488 0.54 -0.4 1,591,343 - Psest_1488 0.54 +1.4 1,591,343 - Psest_1488 0.54 -3.3 1,591,346 + Psest_1488 0.54 +3.3 1,591,346 + Psest_1488 0.54 -3.8 1,591,346 + Psest_1488 0.54 -2.4 1,591,346 + Psest_1488 0.54 +0.8 1,591,346 + Psest_1488 0.54 -0.5 1,591,347 - Psest_1488 0.54 +1.0 1,591,347 - Psest_1488 0.54 -2.3 1,591,350 + Psest_1488 0.55 -3.6 1,591,350 + Psest_1488 0.55 +3.3 1,591,350 + Psest_1488 0.55 +1.5 1,591,350 + Psest_1488 0.55 +0.4 1,591,484 + Psest_1488 0.65 -2.8 1,591,484 + Psest_1488 0.65 +0.7 1,591,484 + Psest_1488 0.65 -1.1 1,591,484 + Psest_1488 0.65 -2.6 1,591,485 - Psest_1488 0.65 -3.9 1,591,485 - Psest_1488 0.65 +0.0 1,591,485 - Psest_1488 0.65 -3.0
Or see this region's nucleotide sequence