Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0013 and Psest_0014 are separated by 26 nucleotides Psest_0014 and Psest_0015 are separated by 25 nucleotides Psest_0015 and Psest_0016 are separated by 146 nucleotides
Psest_0013: Psest_0013 - Lauroyl/myristoyl acyltransferase, at 15,128 to 16,015
_0013
Psest_0014: Psest_0014 - PilZ domain., at 16,042 to 16,308
_0014
Psest_0015: Psest_0015 - Tetratricopeptide repeat., at 16,334 to 16,651
_0015
Psest_0016: Psest_0016 - ectoine/hydroxyectoine ABC transporter, ATP-binding protein, at 16,798 to 17,634
_0016
Position (kb) 16
17 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 15.094 kb on + strand at 15.094 kb on + strand at 15.094 kb on + strand at 15.095 kb on - strand at 15.518 kb on + strand, within Psest_0013 at 15.518 kb on + strand, within Psest_0013 at 15.519 kb on - strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.723 kb on + strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.932 kb on + strand at 15.932 kb on + strand at 15.932 kb on + strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.944 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 16.041 kb on + strand at 16.042 kb on - strand at 16.042 kb on - strand at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.157 kb on - strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.182 kb on - strand, within Psest_0014 at 16.506 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.576 kb on - strand, within Psest_0015 at 16.698 kb on + strand at 16.699 kb on - strand at 16.733 kb on + strand at 16.734 kb on - strand at 16.734 kb on - strand at 16.734 kb on - strand at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 15,094 + +1.7 15,094 + +0.5 15,094 + +0.2 15,095 - +1.2 15,518 + Psest_0013 0.44 +3.0 15,518 + Psest_0013 0.44 +4.9 15,519 - Psest_0013 0.44 +4.0 15,548 + Psest_0013 0.47 +4.4 15,548 + Psest_0013 0.47 +4.1 15,549 - Psest_0013 0.47 +4.2 15,549 - Psest_0013 0.47 +4.9 15,723 + Psest_0013 0.67 +4.5 15,724 - Psest_0013 0.67 +4.5 15,724 - Psest_0013 0.67 +4.6 15,724 - Psest_0013 0.67 +2.9 15,878 + Psest_0013 0.84 +4.1 15,878 + Psest_0013 0.84 +4.3 15,878 + Psest_0013 0.84 +3.2 15,878 + Psest_0013 0.84 +4.4 15,878 + Psest_0013 0.84 +4.5 15,878 + Psest_0013 0.84 +4.3 15,878 + Psest_0013 0.84 +4.1 15,878 + Psest_0013 0.84 +4.3 15,878 + Psest_0013 0.84 +4.4 15,878 + Psest_0013 0.84 +3.8 15,878 + Psest_0013 0.84 +3.9 15,879 - Psest_0013 0.85 +3.9 15,879 - Psest_0013 0.85 +4.0 15,879 - Psest_0013 0.85 +3.7 15,879 - Psest_0013 0.85 +3.9 15,879 - Psest_0013 0.85 +5.3 15,879 - Psest_0013 0.85 +4.5 15,879 - Psest_0013 0.85 +5.0 15,879 - Psest_0013 0.85 +3.7 15,932 + +4.0 15,932 + +4.5 15,932 + +4.6 15,933 - +4.4 15,933 - +4.3 15,933 - +3.6 15,933 - +4.5 15,944 + +4.6 15,956 + +4.7 15,956 + +3.5 15,956 + +5.0 15,956 + +3.3 15,956 + +4.3 15,956 + +5.5 15,956 + +3.9 15,956 + +4.0 15,956 + +3.9 15,956 + +5.8 15,956 + +3.5 15,956 + +5.0 15,956 + +5.3 15,956 + +4.3 15,956 + +3.4 15,956 + +5.1 15,956 + +3.8 15,957 - +3.9 15,957 - +2.6 15,957 - +3.7 15,957 - +3.9 15,957 - +4.3 15,957 - +4.1 15,957 - +3.8 16,041 + -3.1 16,042 - +1.3 16,042 - +0.6 16,156 + Psest_0014 0.43 -1.1 16,156 + Psest_0014 0.43 +1.7 16,156 + Psest_0014 0.43 -2.4 16,156 + Psest_0014 0.43 +1.7 16,157 - Psest_0014 0.43 +1.7 16,181 + Psest_0014 0.52 +0.3 16,181 + Psest_0014 0.52 -2.9 16,182 - Psest_0014 0.52 -2.3 16,506 + Psest_0015 0.54 -1.2 16,575 + Psest_0015 0.76 +1.8 16,575 + Psest_0015 0.76 -1.0 16,575 + Psest_0015 0.76 -1.9 16,576 - Psest_0015 0.76 -3.1 16,698 + +0.8 16,699 - +0.9 16,733 + -0.1 16,734 - -0.6 16,734 - -1.3 16,734 - -0.9 17,287 + Psest_0016 0.58 -2.6 17,287 + Psest_0016 0.58 +0.8 17,287 + Psest_0016 0.58 -2.0 17,287 + Psest_0016 0.58 -0.2 17,287 + Psest_0016 0.58 -2.3 17,287 + Psest_0016 0.58 -0.1 17,287 + Psest_0016 0.58 -0.7 17,288 - Psest_0016 0.59 -0.8 17,288 - Psest_0016 0.59 +2.7 17,288 - Psest_0016 0.59 -3.6
Or see this region's nucleotide sequence