Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1318

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1316 and Psest_1317 are separated by 84 nucleotidesPsest_1317 and Psest_1318 overlap by 4 nucleotidesPsest_1318 and Psest_1319 overlap by 7 nucleotidesPsest_1319 and Psest_1320 are separated by 43 nucleotides Psest_1316: Psest_1316 - Predicted transmembrane sensor domain, at 1,416,625 to 1,417,983 _1316 Psest_1317: Psest_1317 - phosphonate ABC transporter, permease protein PhnE, at 1,418,068 to 1,418,835 _1317 Psest_1318: Psest_1318 - ABC-type phosphate/phosphonate transport system, permease component, at 1,418,832 to 1,419,665 _1318 Psest_1319: Psest_1319 - ABC-type phosphate/phosphonate transport system, ATPase component, at 1,419,659 to 1,420,489 _1319 Psest_1320: Psest_1320 - phosphate/phosphite/phosphonate ABC transporters, periplasmic binding protein, at 1,420,533 to 1,421,384 _1320 Position (kb) 1418 1419 1420Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1417.844 kb on + strand, within Psest_1316at 1417.956 kb on + strandat 1417.957 kb on - strandat 1418.189 kb on + strand, within Psest_1317at 1418.189 kb on + strand, within Psest_1317at 1418.189 kb on + strandat 1418.189 kb on + strand, within Psest_1317at 1418.190 kb on - strand, within Psest_1317at 1418.190 kb on - strand, within Psest_1317at 1418.190 kb on - strand, within Psest_1317at 1418.190 kb on - strand, within Psest_1317at 1418.190 kb on - strand, within Psest_1317at 1418.190 kb on - strand, within Psest_1317at 1418.265 kb on - strand, within Psest_1317at 1418.265 kb on - strand, within Psest_1317at 1418.274 kb on - strand, within Psest_1317at 1419.031 kb on + strand, within Psest_1318at 1419.031 kb on + strand, within Psest_1318at 1419.064 kb on - strand, within Psest_1318at 1419.154 kb on + strand, within Psest_1318at 1419.179 kb on - strand, within Psest_1318at 1419.459 kb on + strand, within Psest_1318at 1419.459 kb on + strand, within Psest_1318at 1419.459 kb on + strand, within Psest_1318at 1419.735 kb on + strandat 1419.735 kb on + strandat 1419.736 kb on - strandat 1419.736 kb on - strandat 1419.736 kb on - strandat 1419.962 kb on + strand, within Psest_1319at 1419.979 kb on - strand, within Psest_1319at 1420.041 kb on + strand, within Psest_1319at 1420.041 kb on + strand, within Psest_1319at 1420.041 kb on + strand, within Psest_1319at 1420.041 kb on + strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.042 kb on - strand, within Psest_1319at 1420.191 kb on - strand, within Psest_1319at 1420.191 kb on - strand, within Psest_1319at 1420.433 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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1,417,844 + Psest_1316 0.90 -1.0
1,417,956 + +0.9
1,417,957 - +0.7
1,418,189 + Psest_1317 0.16 +1.9
1,418,189 + Psest_1317 0.16 +1.2
1,418,189 + +1.2
1,418,189 + Psest_1317 0.16 +0.6
1,418,190 - Psest_1317 0.16 +0.4
1,418,190 - Psest_1317 0.16 +0.7
1,418,190 - Psest_1317 0.16 -1.1
1,418,190 - Psest_1317 0.16 -0.1
1,418,190 - Psest_1317 0.16 +1.2
1,418,190 - Psest_1317 0.16 -0.8
1,418,265 - Psest_1317 0.26 +0.3
1,418,265 - Psest_1317 0.26 +1.9
1,418,274 - Psest_1317 0.27 -1.6
1,419,031 + Psest_1318 0.24 -2.1
1,419,031 + Psest_1318 0.24 -1.1
1,419,064 - Psest_1318 0.28 +0.2
1,419,154 + Psest_1318 0.39 -2.1
1,419,179 - Psest_1318 0.42 -2.1
1,419,459 + Psest_1318 0.75 +0.9
1,419,459 + Psest_1318 0.75 -2.0
1,419,459 + Psest_1318 0.75 -0.9
1,419,735 + -1.1
1,419,735 + +0.0
1,419,736 - +1.4
1,419,736 - -2.3
1,419,736 - +0.2
1,419,962 + Psest_1319 0.36 -0.0
1,419,979 - Psest_1319 0.39 -0.4
1,420,041 + Psest_1319 0.46 -0.5
1,420,041 + Psest_1319 0.46 +0.5
1,420,041 + Psest_1319 0.46 +1.7
1,420,041 + Psest_1319 0.46 -2.0
1,420,042 - Psest_1319 0.46 -0.1
1,420,042 - Psest_1319 0.46 +0.9
1,420,042 - Psest_1319 0.46 +0.8
1,420,042 - Psest_1319 0.46 -0.6
1,420,042 - Psest_1319 0.46 +0.9
1,420,042 - Psest_1319 0.46 -0.8
1,420,042 - Psest_1319 0.46 +0.4
1,420,042 - Psest_1319 0.46 -1.8
1,420,042 - Psest_1319 0.46 +1.2
1,420,191 - Psest_1319 0.64 -0.8
1,420,191 - Psest_1319 0.64 -1.4
1,420,433 - +3.5

Or see this region's nucleotide sequence