Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1286 and Psest_1287 are separated by 143 nucleotides Psest_1287 and Psest_1288 are separated by 109 nucleotides
Psest_1286: Psest_1286 - inosine-5'-monophosphate dehydrogenase, at 1,390,430 to 1,391,899
_1286
Psest_1287: Psest_1287 - GMP synthase (glutamine-hydrolyzing), C-terminal domain or B subunit/GMP synthase (glutamine-hydrolyzing), N-terminal domain or A subunit, at 1,392,043 to 1,393,623
_1287
Psest_1288: Psest_1288 - Putative multicopper oxidases, at 1,393,733 to 1,395,103
_1288
Position (kb)
1392
1393
1394 Strain fitness (log2 ratio)
-2
-1
0
1 at 1391.980 kb on + strand at 1393.662 kb on - strand at 1393.850 kb on + strand at 1393.850 kb on + strand at 1393.850 kb on + strand at 1393.917 kb on - strand, within Psest_1288 at 1394.058 kb on - strand, within Psest_1288 at 1394.061 kb on + strand, within Psest_1288 at 1394.400 kb on + strand, within Psest_1288 at 1394.401 kb on - strand, within Psest_1288 at 1394.417 kb on - strand, within Psest_1288 at 1394.417 kb on - strand, within Psest_1288 at 1394.462 kb on + strand, within Psest_1288 at 1394.462 kb on + strand, within Psest_1288 at 1394.462 kb on + strand, within Psest_1288 at 1394.463 kb on - strand, within Psest_1288 at 1394.463 kb on - strand, within Psest_1288 at 1394.463 kb on - strand, within Psest_1288
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,391,980 + +1.6 1,393,662 - -2.3 1,393,850 + -1.5 1,393,850 + +0.6 1,393,850 + +0.3 1,393,917 - Psest_1288 0.13 +0.7 1,394,058 - Psest_1288 0.24 -0.0 1,394,061 + Psest_1288 0.24 +1.1 1,394,400 + Psest_1288 0.49 -1.6 1,394,401 - Psest_1288 0.49 +1.0 1,394,417 - Psest_1288 0.50 -0.4 1,394,417 - Psest_1288 0.50 -0.3 1,394,462 + Psest_1288 0.53 +1.1 1,394,462 + Psest_1288 0.53 +0.9 1,394,462 + Psest_1288 0.53 +1.4 1,394,463 - Psest_1288 0.53 +1.2 1,394,463 - Psest_1288 0.53 +0.8 1,394,463 - Psest_1288 0.53 -1.4
Or see this region's nucleotide sequence