Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0124

Experiment: Polymyxin B sulfate 0.0001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Polymyxin B sulfate 0.0001 mg/ml
119,539	-		Psest_0123	0.54	+1.3
119,539	-		Psest_0123	0.54	-2.3
119,539	-		Psest_0123	0.54	+2.2
119,744	+		Psest_0123	0.64	-1.0
119,817	-		Psest_0123	0.67	-2.5
119,912	+		Psest_0123	0.72	-3.1
119,996	+		Psest_0123	0.76	-0.1
119,996	+		Psest_0123	0.76	+0.2
120,053	+		Psest_0123	0.79	-0.3
120,053	+		Psest_0123	0.79	-0.0
120,065	+		Psest_0123	0.79	-0.4
120,065	+		Psest_0123	0.79	-0.0
120,207	-		Psest_0123	0.86	-0.3
120,280	+		Psest_0123	0.89	+0.3
120,280	+		Psest_0123	0.89	+1.3
120,280	+		Psest_0123	0.89	-1.0
120,280	+		Psest_0123	0.89	-1.9
120,280	+		Psest_0123	0.89	-0.6
120,280	+		Psest_0123	0.89	-0.7
120,280	+		Psest_0123	0.89	-1.9
120,280	+		Psest_0123	0.89	+1.3
120,280	+		Psest_0123	0.89	-0.1
120,281	-		Psest_0123	0.89	+0.7
120,519	-				-0.7
120,519	-				+1.6
120,519	-				+0.7
120,519	-				+1.4
120,715	+		Psest_0124	0.13	+1.3
120,715	+		Psest_0124	0.13	-0.3
120,715	+		Psest_0124	0.13	+1.6
120,715	+		Psest_0124	0.13	+3.5
120,715	+		Psest_0124	0.13	+0.3
120,715	+		Psest_0124	0.13	-0.7
120,716	-		Psest_0124	0.13	+1.1
120,716	-		Psest_0124	0.13	+2.6
120,716	-		Psest_0124	0.13	-3.2
120,716	-		Psest_0124	0.13	-1.3
120,716	-		Psest_0124	0.13	-1.7
120,962	+		Psest_0124	0.29	-0.0
121,276	+		Psest_0124	0.50	-1.0
121,276	+		Psest_0124	0.50	+0.1
121,407	-		Psest_0124	0.59	-0.0
121,755	-		Psest_0124	0.82	+1.5
121,874	+		Psest_0124	0.89	+1.0
121,875	-		Psest_0124	0.89	+2.0
121,875	-		Psest_0124	0.89	+0.9
121,875	-		Psest_0124	0.89	+0.7
121,900	+				+2.9
121,900	+				-0.3
121,900	+				+0.9
122,331	+		Psest_0125	0.11	-0.3
122,331	+		Psest_0125	0.11	+0.6
122,332	-		Psest_0125	0.11	+1.3
122,332	-		Psest_0125	0.11	+1.8
122,334	+		Psest_0125	0.11	+1.3
122,335	-		Psest_0125	0.12	+0.1
122,556	+		Psest_0125	0.20	+0.9
122,556	+		Psest_0125	0.20	-0.7
122,557	-		Psest_0125	0.20	-1.3
122,557	-		Psest_0125	0.20	+0.3
122,557	-		Psest_0125	0.20	-0.3
122,616	+		Psest_0125	0.22	+2.3
122,728	+		Psest_0125	0.27	+2.6
122,728	+		Psest_0125	0.27	+1.3
122,728	+				+1.3
122,728	+		Psest_0125	0.27	-0.3
122,728	+		Psest_0125	0.27	+1.3
122,729	-		Psest_0125	0.27	-1.5
122,729	-		Psest_0125	0.27	-0.3