Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1269 and Psest_1270 are separated by 11 nucleotides Psest_1270 and Psest_1271 are separated by 55 nucleotides Psest_1271 and Psest_1272 overlap by 15 nucleotides Psest_1272 and Psest_1273 overlap by 4 nucleotides
Psest_1269: Psest_1269 - Predicted amidohydrolase, at 1,374,769 to 1,375,563
_1269
Psest_1270: Psest_1270 - hypothetical protein, at 1,375,575 to 1,375,766
_1270
Psest_1271: Psest_1271 - Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family, at 1,375,822 to 1,376,355
_1271
Psest_1272: Psest_1272 - Protein of Unknown function (DUF2784)., at 1,376,341 to 1,376,712
_1272
Psest_1273: Psest_1273 - Predicted membrane protein, at 1,376,709 to 1,377,344
_1273
Position (kb)
1375
1376
1377 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1374.976 kb on + strand, within Psest_1269 at 1374.976 kb on + strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1375.016 kb on + strand, within Psest_1269 at 1375.017 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.150 kb on + strand, within Psest_1269 at 1375.150 kb on + strand, within Psest_1269 at 1375.151 kb on - strand, within Psest_1269 at 1375.274 kb on - strand, within Psest_1269 at 1375.351 kb on + strand, within Psest_1269 at 1375.352 kb on - strand, within Psest_1269 at 1375.352 kb on - strand, within Psest_1269 at 1375.567 kb on + strand at 1375.568 kb on - strand at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.613 kb on - strand, within Psest_1270 at 1375.613 kb on - strand, within Psest_1270 at 1375.640 kb on - strand, within Psest_1270 at 1375.760 kb on + strand at 1375.852 kb on + strand at 1375.852 kb on + strand at 1375.852 kb on + strand at 1375.852 kb on + strand at 1376.122 kb on - strand, within Psest_1271 at 1376.122 kb on - strand, within Psest_1271 at 1376.280 kb on + strand, within Psest_1271 at 1376.280 kb on + strand, within Psest_1271 at 1376.280 kb on + strand, within Psest_1271 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.381 kb on + strand, within Psest_1272 at 1376.385 kb on + strand, within Psest_1272 at 1376.385 kb on + strand, within Psest_1272 at 1376.385 kb on + strand, within Psest_1272 at 1376.385 kb on + strand, within Psest_1272 at 1376.423 kb on + strand, within Psest_1272 at 1376.423 kb on + strand, within Psest_1272 at 1376.423 kb on + strand, within Psest_1272 at 1376.423 kb on + strand, within Psest_1272 at 1376.423 kb on + strand, within Psest_1272 at 1376.424 kb on - strand, within Psest_1272 at 1376.424 kb on - strand, within Psest_1272 at 1376.424 kb on - strand, within Psest_1272 at 1376.471 kb on + strand, within Psest_1272 at 1376.605 kb on + strand, within Psest_1272 at 1376.605 kb on + strand, within Psest_1272 at 1376.605 kb on + strand, within Psest_1272 at 1376.606 kb on - strand, within Psest_1272 at 1376.705 kb on + strand at 1376.706 kb on - strand at 1376.914 kb on + strand, within Psest_1273 at 1376.915 kb on - strand, within Psest_1273 at 1377.055 kb on + strand, within Psest_1273 at 1377.056 kb on - strand, within Psest_1273 at 1377.056 kb on - strand, within Psest_1273 at 1377.062 kb on + strand, within Psest_1273 at 1377.062 kb on + strand, within Psest_1273 at 1377.115 kb on + strand, within Psest_1273 at 1377.116 kb on - strand, within Psest_1273 at 1377.119 kb on + strand, within Psest_1273 at 1377.119 kb on + strand, within Psest_1273 at 1377.120 kb on - strand, within Psest_1273 at 1377.120 kb on - strand, within Psest_1273 at 1377.120 kb on - strand, within Psest_1273 at 1377.120 kb on - strand, within Psest_1273 at 1377.121 kb on + strand, within Psest_1273 at 1377.121 kb on + strand, within Psest_1273 at 1377.121 kb on + strand, within Psest_1273 at 1377.122 kb on - strand, within Psest_1273 at 1377.166 kb on - strand, within Psest_1273 at 1377.169 kb on + strand, within Psest_1273 at 1377.169 kb on + strand, within Psest_1273 at 1377.169 kb on + strand, within Psest_1273 at 1377.170 kb on - strand, within Psest_1273 at 1377.170 kb on - strand, within Psest_1273 at 1377.170 kb on - strand, within Psest_1273 at 1377.204 kb on - strand, within Psest_1273 at 1377.204 kb on - strand, within Psest_1273 at 1377.204 kb on - strand, within Psest_1273 at 1377.204 kb on - strand, within Psest_1273 at 1377.204 kb on - strand, within Psest_1273 at 1377.263 kb on + strand, within Psest_1273 at 1377.269 kb on - strand, within Psest_1273 at 1377.344 kb on + strand at 1377.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,374,976 + Psest_1269 0.26 +1.5 1,374,976 + Psest_1269 0.26 +0.7 1,374,977 - Psest_1269 0.26 +0.1 1,374,977 - Psest_1269 0.26 +0.7 1,374,977 - Psest_1269 0.26 +1.2 1,375,016 + Psest_1269 0.31 +0.7 1,375,017 - Psest_1269 0.31 +0.1 1,375,106 - Psest_1269 0.42 -0.0 1,375,106 - Psest_1269 0.42 -1.0 1,375,106 - Psest_1269 0.42 +1.0 1,375,150 + Psest_1269 0.48 -2.7 1,375,150 + Psest_1269 0.48 +0.2 1,375,151 - Psest_1269 0.48 +0.6 1,375,274 - Psest_1269 0.64 +0.6 1,375,351 + Psest_1269 0.73 +0.6 1,375,352 - Psest_1269 0.73 -1.1 1,375,352 - Psest_1269 0.73 +1.9 1,375,567 + -0.8 1,375,568 - -1.4 1,375,612 + Psest_1270 0.19 -0.9 1,375,612 + Psest_1270 0.19 +0.8 1,375,612 + Psest_1270 0.19 +1.1 1,375,612 + Psest_1270 0.19 +0.4 1,375,613 - Psest_1270 0.20 -1.1 1,375,613 - Psest_1270 0.20 +2.7 1,375,640 - Psest_1270 0.34 +1.3 1,375,760 + +0.9 1,375,852 + -2.5 1,375,852 + -0.8 1,375,852 + +0.0 1,375,852 + +0.9 1,376,122 - Psest_1271 0.56 -0.5 1,376,122 - Psest_1271 0.56 +1.1 1,376,280 + Psest_1271 0.86 +1.1 1,376,280 + Psest_1271 0.86 -1.6 1,376,280 + Psest_1271 0.86 -0.3 1,376,381 + Psest_1272 0.11 -0.1 1,376,381 + Psest_1272 0.11 -0.2 1,376,381 + Psest_1272 0.11 -0.5 1,376,381 + Psest_1272 0.11 -1.4 1,376,381 + Psest_1272 0.11 -0.2 1,376,381 + Psest_1272 0.11 -2.6 1,376,381 + Psest_1272 0.11 -2.5 1,376,381 + Psest_1272 0.11 +1.8 1,376,381 + Psest_1272 0.11 -2.2 1,376,381 + Psest_1272 0.11 +0.5 1,376,381 + Psest_1272 0.11 -1.4 1,376,381 + Psest_1272 0.11 -1.9 1,376,381 + Psest_1272 0.11 +0.5 1,376,381 + Psest_1272 0.11 +0.5 1,376,381 + Psest_1272 0.11 +1.2 1,376,381 + Psest_1272 0.11 -3.2 1,376,381 + Psest_1272 0.11 +1.0 1,376,385 + Psest_1272 0.12 -1.6 1,376,385 + Psest_1272 0.12 +0.9 1,376,385 + Psest_1272 0.12 -0.0 1,376,385 + Psest_1272 0.12 -0.8 1,376,423 + Psest_1272 0.22 +1.8 1,376,423 + Psest_1272 0.22 +1.2 1,376,423 + Psest_1272 0.22 +0.7 1,376,423 + Psest_1272 0.22 -1.0 1,376,423 + Psest_1272 0.22 -2.9 1,376,424 - Psest_1272 0.22 -3.9 1,376,424 - Psest_1272 0.22 +2.5 1,376,424 - Psest_1272 0.22 -2.4 1,376,471 + Psest_1272 0.35 -1.6 1,376,605 + Psest_1272 0.71 -3.5 1,376,605 + Psest_1272 0.71 +1.7 1,376,605 + Psest_1272 0.71 -4.9 1,376,606 - Psest_1272 0.71 -2.6 1,376,705 + +1.5 1,376,706 - -0.1 1,376,914 + Psest_1273 0.32 -3.4 1,376,915 - Psest_1273 0.32 +1.8 1,377,055 + Psest_1273 0.54 -3.3 1,377,056 - Psest_1273 0.55 -0.1 1,377,056 - Psest_1273 0.55 +0.1 1,377,062 + Psest_1273 0.56 +0.5 1,377,062 + Psest_1273 0.56 +0.6 1,377,115 + Psest_1273 0.64 -1.1 1,377,116 - Psest_1273 0.64 +1.3 1,377,119 + Psest_1273 0.64 -1.3 1,377,119 + Psest_1273 0.64 +0.7 1,377,120 - Psest_1273 0.65 +0.5 1,377,120 - Psest_1273 0.65 -0.7 1,377,120 - Psest_1273 0.65 -3.5 1,377,120 - Psest_1273 0.65 -0.9 1,377,121 + Psest_1273 0.65 +2.1 1,377,121 + Psest_1273 0.65 +0.6 1,377,121 + Psest_1273 0.65 +0.7 1,377,122 - Psest_1273 0.65 +1.0 1,377,166 - Psest_1273 0.72 +0.3 1,377,169 + Psest_1273 0.72 +3.0 1,377,169 + Psest_1273 0.72 +1.6 1,377,169 + Psest_1273 0.72 +0.9 1,377,170 - Psest_1273 0.72 +1.5 1,377,170 - Psest_1273 0.72 +0.3 1,377,170 - Psest_1273 0.72 -0.6 1,377,204 - Psest_1273 0.78 -1.8 1,377,204 - Psest_1273 0.78 +0.2 1,377,204 - Psest_1273 0.78 -0.8 1,377,204 - Psest_1273 0.78 -2.9 1,377,204 - Psest_1273 0.78 -1.1 1,377,263 + Psest_1273 0.87 +0.5 1,377,269 - Psest_1273 0.88 -0.5 1,377,344 + -0.4 1,377,345 - +3.8
Or see this region's nucleotide sequence