Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1255 and Psest_1256 are separated by 5 nucleotides Psest_1256 and Psest_1257 are separated by 12 nucleotides Psest_1257 and Psest_1258 are separated by 165 nucleotides Psest_1258 and Psest_1259 are separated by 19 nucleotides
Psest_1255: Psest_1255 - Fe-S protein assembly chaperone HscA, at 1,360,871 to 1,362,733
_1255
Psest_1256: Psest_1256 - ferredoxin, 2Fe-2S type, ISC system, at 1,362,739 to 1,363,077
_1256
Psest_1257: Psest_1257 - FeS assembly protein IscX, at 1,363,090 to 1,363,290
_1257
Psest_1258: Psest_1258 - Nucleoside diphosphate kinase, at 1,363,456 to 1,363,887
_1258
Psest_1259: Psest_1259 - 23S rRNA m2A2503 methyltransferase, at 1,363,907 to 1,365,055
_1259
Position (kb)
1363
1364 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1362.559 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.098 kb on + strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.099 kb on - strand at 1363.374 kb on + strand at 1363.380 kb on + strand at 1363.381 kb on - strand at 1363.403 kb on + strand at 1364.166 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.201 kb on + strand, within Psest_1259 at 1364.229 kb on + strand, within Psest_1259 at 1364.229 kb on + strand, within Psest_1259 at 1364.299 kb on + strand, within Psest_1259 at 1364.458 kb on + strand, within Psest_1259 at 1364.458 kb on + strand, within Psest_1259 at 1364.485 kb on + strand, within Psest_1259 at 1364.593 kb on + strand, within Psest_1259 at 1364.633 kb on + strand, within Psest_1259 at 1364.644 kb on + strand, within Psest_1259 at 1364.644 kb on + strand, within Psest_1259 at 1364.644 kb on + strand, within Psest_1259 at 1364.669 kb on + strand, within Psest_1259 at 1364.671 kb on + strand, within Psest_1259
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,362,559 + +2.0 1,363,098 + +1.0 1,363,098 + +1.4 1,363,098 + -0.3 1,363,098 + +1.9 1,363,098 + -0.5 1,363,098 + -1.0 1,363,098 + -1.1 1,363,098 + +1.4 1,363,099 - +0.9 1,363,099 - -0.6 1,363,099 - -0.7 1,363,099 - +1.6 1,363,099 - +0.8 1,363,099 - -0.6 1,363,099 - -3.2 1,363,099 - +1.7 1,363,099 - +1.7 1,363,374 + -2.9 1,363,380 + -1.6 1,363,381 - -2.6 1,363,403 + +0.0 1,364,166 + Psest_1259 0.23 -0.8 1,364,201 + Psest_1259 0.26 -1.6 1,364,201 + Psest_1259 0.26 -1.7 1,364,201 + Psest_1259 0.26 -1.6 1,364,201 + Psest_1259 0.26 -1.4 1,364,201 + Psest_1259 0.26 -1.6 1,364,201 + Psest_1259 0.26 +0.2 1,364,201 + Psest_1259 0.26 -3.8 1,364,201 + Psest_1259 0.26 -2.4 1,364,229 + Psest_1259 0.28 -2.7 1,364,229 + Psest_1259 0.28 -2.4 1,364,299 + Psest_1259 0.34 -2.9 1,364,458 + Psest_1259 0.48 +1.4 1,364,458 + Psest_1259 0.48 -3.4 1,364,485 + Psest_1259 0.50 -0.6 1,364,593 + Psest_1259 0.60 -2.5 1,364,633 + Psest_1259 0.63 +1.3 1,364,644 + Psest_1259 0.64 +1.0 1,364,644 + Psest_1259 0.64 +1.4 1,364,644 + Psest_1259 0.64 -2.5 1,364,669 + Psest_1259 0.66 +0.5 1,364,671 + Psest_1259 0.66 +4.5
Or see this region's nucleotide sequence