Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1214 and Psest_1215 are separated by 114 nucleotides Psest_1215 and Psest_1216 are separated by 205 nucleotides
Psest_1214: Psest_1214 - Cytosine deaminase and related metal-dependent hydrolases, at 1,319,824 to 1,321,179
_1214
Psest_1215: Psest_1215 - Short-chain dehydrogenase involved in D-alanine esterification of lipoteichoic acid and wall teichoic acid (D-alanine transfer protein), at 1,321,294 to 1,321,743
_1215
Psest_1216: Psest_1216 - ATPase, P-type (transporting), HAD superfamily, subfamily IC, at 1,321,949 to 1,324,663
_1216
Position (kb)
1321
1322 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1320.413 kb on + strand, within Psest_1214 at 1320.413 kb on + strand, within Psest_1214 at 1320.483 kb on + strand, within Psest_1214 at 1320.484 kb on - strand, within Psest_1214 at 1320.493 kb on + strand, within Psest_1214 at 1320.493 kb on + strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1320.526 kb on - strand, within Psest_1214 at 1321.226 kb on + strand at 1321.227 kb on - strand at 1321.285 kb on + strand at 1321.286 kb on - strand at 1321.286 kb on - strand at 1321.286 kb on - strand at 1321.286 kb on - strand at 1321.436 kb on + strand, within Psest_1215 at 1321.436 kb on + strand, within Psest_1215 at 1321.436 kb on + strand, within Psest_1215 at 1321.437 kb on - strand, within Psest_1215 at 1321.437 kb on - strand, within Psest_1215 at 1321.880 kb on + strand at 1321.881 kb on - strand at 1321.892 kb on + strand at 1321.892 kb on + strand at 1321.892 kb on + strand at 1321.892 kb on + strand at 1321.893 kb on - strand at 1321.893 kb on - strand at 1321.893 kb on - strand at 1321.893 kb on - strand at 1321.899 kb on + strand at 1321.900 kb on - strand at 1321.900 kb on - strand at 1322.136 kb on + strand at 1322.136 kb on + strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.153 kb on + strand at 1322.154 kb on - strand at 1322.206 kb on + strand at 1322.206 kb on + strand at 1322.229 kb on + strand, within Psest_1216 at 1322.229 kb on + strand, within Psest_1216 at 1322.230 kb on - strand, within Psest_1216 at 1322.230 kb on - strand, within Psest_1216
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,320,413 + Psest_1214 0.43 -1.2 1,320,413 + Psest_1214 0.43 +0.8 1,320,483 + Psest_1214 0.49 -0.9 1,320,484 - Psest_1214 0.49 -1.4 1,320,493 + Psest_1214 0.49 -2.3 1,320,493 + Psest_1214 0.49 +0.4 1,320,526 - Psest_1214 0.52 -1.8 1,320,526 - Psest_1214 0.52 +0.8 1,320,526 - Psest_1214 0.52 -0.8 1,320,526 - Psest_1214 0.52 -2.1 1,320,526 - Psest_1214 0.52 -2.0 1,321,226 + +1.2 1,321,227 - +0.2 1,321,285 + +0.2 1,321,286 - -0.4 1,321,286 - +1.2 1,321,286 - -0.5 1,321,286 - -0.6 1,321,436 + Psest_1215 0.32 +0.2 1,321,436 + Psest_1215 0.32 -1.4 1,321,436 + Psest_1215 0.32 -1.2 1,321,437 - Psest_1215 0.32 -1.1 1,321,437 - Psest_1215 0.32 -0.8 1,321,880 + -0.8 1,321,881 - +1.9 1,321,892 + +0.2 1,321,892 + +0.3 1,321,892 + +0.2 1,321,892 + -0.4 1,321,893 - -0.4 1,321,893 - +2.0 1,321,893 - +0.0 1,321,893 - +1.5 1,321,899 + +0.2 1,321,900 - +1.2 1,321,900 - -0.6 1,322,136 + +1.6 1,322,136 + +1.2 1,322,137 - +0.2 1,322,137 - +1.0 1,322,137 - +3.2 1,322,137 - -1.7 1,322,137 - -0.4 1,322,153 + -0.9 1,322,154 - +0.2 1,322,206 + -2.6 1,322,206 + +1.6 1,322,229 + Psest_1216 0.10 -1.1 1,322,229 + Psest_1216 0.10 -0.6 1,322,230 - Psest_1216 0.10 -0.1 1,322,230 - Psest_1216 0.10 +0.1
Or see this region's nucleotide sequence