Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0112 and Psest_0113 are separated by 58 nucleotides Psest_0113 and Psest_0114 overlap by 4 nucleotides Psest_0114 and Psest_0115 are separated by 191 nucleotides Psest_0115 and Psest_0116 are separated by 16 nucleotides
Psest_0112: Psest_0112 - Transcriptional regulator, at 110,797 to 111,714
_0112
Psest_0113: Psest_0113 - Uncharacterized protein conserved in bacteria, at 111,773 to 112,414
_0113
Psest_0114: Psest_0114 - RNA polymerase sigma factor, sigma-70 family, at 112,411 to 112,980
_0114
Psest_0115: Psest_0115 - Predicted integral membrane protein, at 113,172 to 113,444
_0115
Psest_0116: Psest_0116 - Uncharacterized protein conserved in bacteria, at 113,461 to 114,288
_0116
Position (kb) 112
113 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.744 kb on + strand at 111.745 kb on - strand at 111.806 kb on - strand at 111.841 kb on - strand, within Psest_0113 at 112.281 kb on + strand, within Psest_0113 at 112.281 kb on + strand, within Psest_0113 at 112.577 kb on - strand, within Psest_0114 at 112.789 kb on + strand, within Psest_0114 at 112.789 kb on + strand, within Psest_0114 at 112.968 kb on + strand at 112.969 kb on - strand at 112.969 kb on - strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.384 kb on + strand, within Psest_0115 at 113.448 kb on + strand at 113.449 kb on - strand at 113.484 kb on - strand at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 111,512 + Psest_0112 0.78 +0.3 111,512 + Psest_0112 0.78 +1.3 111,512 + Psest_0112 0.78 +1.7 111,512 + Psest_0112 0.78 -0.7 111,512 + Psest_0112 0.78 +0.3 111,512 + Psest_0112 0.78 +1.3 111,744 + +0.3 111,745 - -1.3 111,806 - -2.4 111,841 - Psest_0113 0.11 -1.4 112,281 + Psest_0113 0.79 -0.9 112,281 + Psest_0113 0.79 +0.1 112,577 - Psest_0114 0.29 +0.3 112,789 + Psest_0114 0.66 -1.4 112,789 + Psest_0114 0.66 -2.9 112,968 + -0.3 112,969 - +0.3 112,969 - +0.1 113,112 + -0.3 113,112 + +0.3 113,112 + -0.3 113,112 + +0.9 113,113 - +0.5 113,113 - +1.7 113,113 - -0.7 113,113 - -0.7 113,113 - -0.6 113,384 + Psest_0115 0.78 +0.2 113,448 + +1.9 113,449 - +0.3 113,484 - -0.7 113,563 + Psest_0116 0.12 +0.9 113,563 + Psest_0116 0.12 +1.0 113,563 + Psest_0116 0.12 -1.7 113,563 + Psest_0116 0.12 -0.0 113,563 + Psest_0116 0.12 +0.6 113,563 + Psest_0116 0.12 -0.5 113,564 - Psest_0116 0.12 +1.0 113,564 - Psest_0116 0.12 +1.1 113,564 - Psest_0116 0.12 +0.3 113,564 - Psest_0116 0.12 -0.0 113,564 - Psest_0116 0.12 +0.4 113,564 - Psest_0116 0.12 -1.9 113,564 - Psest_0116 0.12 -1.5 113,564 - Psest_0116 0.12 +0.3 113,767 + Psest_0116 0.37 +2.2 113,767 + Psest_0116 0.37 -0.3 113,767 + Psest_0116 0.37 -0.7 113,767 + Psest_0116 0.37 -1.7 113,767 + Psest_0116 0.37 +0.8 113,767 + Psest_0116 0.37 -0.9 113,768 - Psest_0116 0.37 +0.3 113,768 - Psest_0116 0.37 +0.9 113,768 - Psest_0116 0.37 -0.8 113,768 - Psest_0116 0.37 +2.4 113,768 - Psest_0116 0.37 +0.1 113,768 - Psest_0116 0.37 +0.7 113,768 - Psest_0116 0.37 +0.9 113,888 - Psest_0116 0.52 -0.3 113,888 - Psest_0116 0.52 +1.3
Or see this region's nucleotide sequence