Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1059 and Psest_1060 are separated by 180 nucleotides Psest_1060 and Psest_1061 are separated by 16 nucleotides Psest_1061 and Psest_1062 are separated by 80 nucleotides Psest_1062 and Psest_1063 overlap by 1 nucleotides
Psest_1059: Psest_1059 - hypothetical protein, at 1,148,663 to 1,149,205
_1059
Psest_1060: Psest_1060 - trehalose-phosphatase, at 1,149,386 to 1,150,162
_1060
Psest_1061: Psest_1061 - alpha,alpha-trehalose-phosphate synthase [UDP-forming], at 1,150,179 to 1,151,594
_1061
Psest_1062: Psest_1062 - Carbonic anhydrase, at 1,151,675 to 1,152,319
_1062
Psest_1063: Psest_1063 - Predicted permease, DMT superfamily, at 1,152,319 to 1,153,218
_1063
Position (kb)
1150
1151
1152 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1149.378 kb on + strand at 1149.379 kb on - strand at 1149.379 kb on - strand at 1150.161 kb on - strand at 1150.172 kb on + strand at 1150.172 kb on + strand at 1150.173 kb on - strand at 1150.189 kb on + strand at 1150.190 kb on - strand at 1150.190 kb on - strand at 1150.190 kb on - strand at 1150.192 kb on + strand at 1150.193 kb on - strand at 1150.263 kb on + strand at 1150.264 kb on - strand at 1150.362 kb on + strand, within Psest_1061 at 1150.362 kb on + strand, within Psest_1061 at 1150.362 kb on + strand, within Psest_1061 at 1150.362 kb on + strand, within Psest_1061 at 1150.362 kb on + strand, within Psest_1061 at 1150.362 kb on + strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.363 kb on - strand, within Psest_1061 at 1150.392 kb on + strand, within Psest_1061 at 1150.393 kb on - strand, within Psest_1061 at 1150.401 kb on + strand, within Psest_1061 at 1150.405 kb on - strand, within Psest_1061 at 1150.710 kb on + strand, within Psest_1061 at 1150.711 kb on - strand, within Psest_1061 at 1150.711 kb on - strand, within Psest_1061 at 1150.714 kb on - strand, within Psest_1061 at 1150.764 kb on + strand, within Psest_1061 at 1150.764 kb on + strand, within Psest_1061 at 1150.764 kb on + strand, within Psest_1061 at 1150.764 kb on + strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.765 kb on - strand, within Psest_1061 at 1150.893 kb on + strand, within Psest_1061 at 1150.894 kb on - strand, within Psest_1061 at 1151.094 kb on + strand, within Psest_1061 at 1151.094 kb on + strand, within Psest_1061 at 1151.094 kb on + strand, within Psest_1061 at 1151.094 kb on + strand, within Psest_1061 at 1151.094 kb on + strand, within Psest_1061 at 1151.178 kb on + strand, within Psest_1061 at 1151.178 kb on + strand, within Psest_1061 at 1151.179 kb on - strand, within Psest_1061 at 1151.193 kb on + strand, within Psest_1061 at 1151.193 kb on + strand, within Psest_1061 at 1151.194 kb on - strand, within Psest_1061 at 1151.194 kb on - strand, within Psest_1061 at 1151.194 kb on - strand, within Psest_1061 at 1151.194 kb on - strand, within Psest_1061 at 1151.286 kb on + strand, within Psest_1061 at 1151.286 kb on + strand, within Psest_1061 at 1151.287 kb on - strand, within Psest_1061 at 1151.287 kb on - strand, within Psest_1061 at 1151.287 kb on - strand, within Psest_1061 at 1151.641 kb on + strand at 1151.642 kb on - strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.672 kb on + strand at 1151.673 kb on - strand at 1151.673 kb on - strand at 1151.673 kb on - strand at 1151.673 kb on - strand at 1151.673 kb on - strand at 1151.673 kb on - strand at 1151.673 kb on - strand at 1151.673 kb on - strand at 1151.783 kb on + strand, within Psest_1062 at 1152.053 kb on + strand, within Psest_1062 at 1152.053 kb on + strand, within Psest_1062 at 1152.054 kb on - strand, within Psest_1062 at 1152.054 kb on - strand, within Psest_1062 at 1152.054 kb on - strand, within Psest_1062 at 1152.054 kb on - strand, within Psest_1062 at 1152.054 kb on - strand, within Psest_1062 at 1152.057 kb on - strand, within Psest_1062 at 1152.218 kb on + strand, within Psest_1062 at 1152.219 kb on - strand, within Psest_1062 at 1152.219 kb on - strand, within Psest_1062 at 1152.219 kb on - strand, within Psest_1062 at 1152.219 kb on - strand, within Psest_1062 at 1152.406 kb on + strand at 1152.406 kb on + strand at 1152.406 kb on + strand at 1152.406 kb on + strand at 1152.406 kb on + strand at 1152.406 kb on + strand at 1152.406 kb on + strand at 1152.407 kb on - strand at 1152.407 kb on - strand at 1152.407 kb on - strand at 1152.407 kb on - strand at 1152.407 kb on - strand at 1152.407 kb on - strand at 1152.407 kb on - strand at 1152.407 kb on - strand at 1152.565 kb on + strand, within Psest_1063 at 1152.565 kb on + strand, within Psest_1063 at 1152.565 kb on + strand, within Psest_1063 at 1152.566 kb on - strand, within Psest_1063 at 1152.566 kb on - strand, within Psest_1063 at 1152.566 kb on - strand, within Psest_1063 at 1152.580 kb on + strand, within Psest_1063 at 1152.581 kb on - strand, within Psest_1063 at 1152.581 kb on - strand, within Psest_1063
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,149,378 + +0.1 1,149,379 - +0.0 1,149,379 - +0.6 1,150,161 - -0.0 1,150,172 + -0.9 1,150,172 + -1.4 1,150,173 - -0.0 1,150,189 + +0.0 1,150,190 - -0.1 1,150,190 - -0.2 1,150,190 - -1.9 1,150,192 + -2.3 1,150,193 - +3.4 1,150,263 + -0.2 1,150,264 - -1.0 1,150,362 + Psest_1061 0.13 -0.3 1,150,362 + Psest_1061 0.13 -1.9 1,150,362 + Psest_1061 0.13 -2.0 1,150,362 + Psest_1061 0.13 -1.2 1,150,362 + Psest_1061 0.13 +1.3 1,150,362 + Psest_1061 0.13 +2.1 1,150,363 - Psest_1061 0.13 -0.3 1,150,363 - Psest_1061 0.13 -1.1 1,150,363 - Psest_1061 0.13 +0.0 1,150,363 - Psest_1061 0.13 -1.4 1,150,363 - Psest_1061 0.13 -1.4 1,150,363 - Psest_1061 0.13 -2.4 1,150,363 - Psest_1061 0.13 -2.4 1,150,363 - Psest_1061 0.13 +1.6 1,150,363 - Psest_1061 0.13 -0.9 1,150,392 + Psest_1061 0.15 -3.0 1,150,393 - Psest_1061 0.15 -0.6 1,150,401 + Psest_1061 0.16 -2.6 1,150,405 - Psest_1061 0.16 -1.2 1,150,710 + Psest_1061 0.38 -1.4 1,150,711 - Psest_1061 0.38 -0.1 1,150,711 - Psest_1061 0.38 +2.1 1,150,714 - Psest_1061 0.38 +1.3 1,150,764 + Psest_1061 0.41 -2.0 1,150,764 + Psest_1061 0.41 +0.9 1,150,764 + Psest_1061 0.41 +1.7 1,150,764 + Psest_1061 0.41 -1.2 1,150,765 - Psest_1061 0.41 +2.5 1,150,765 - Psest_1061 0.41 -0.9 1,150,765 - Psest_1061 0.41 -0.2 1,150,765 - Psest_1061 0.41 -0.3 1,150,765 - Psest_1061 0.41 +0.1 1,150,765 - Psest_1061 0.41 +1.6 1,150,765 - Psest_1061 0.41 -1.2 1,150,765 - Psest_1061 0.41 -0.4 1,150,893 + Psest_1061 0.50 +1.4 1,150,894 - Psest_1061 0.50 -0.9 1,151,094 + Psest_1061 0.65 +0.8 1,151,094 + Psest_1061 0.65 -0.4 1,151,094 + Psest_1061 0.65 -0.0 1,151,094 + Psest_1061 0.65 +1.1 1,151,094 + Psest_1061 0.65 -1.4 1,151,178 + Psest_1061 0.71 +1.4 1,151,178 + Psest_1061 0.71 +2.3 1,151,179 - Psest_1061 0.71 -0.4 1,151,193 + Psest_1061 0.72 +1.1 1,151,193 + Psest_1061 0.72 -0.6 1,151,194 - Psest_1061 0.72 -1.4 1,151,194 - Psest_1061 0.72 -0.9 1,151,194 - Psest_1061 0.72 -1.9 1,151,194 - Psest_1061 0.72 +0.1 1,151,286 + Psest_1061 0.78 -0.4 1,151,286 + Psest_1061 0.78 -0.3 1,151,287 - Psest_1061 0.78 -0.2 1,151,287 - Psest_1061 0.78 +0.1 1,151,287 - Psest_1061 0.78 +0.3 1,151,641 + -1.7 1,151,642 - -1.2 1,151,672 + -0.9 1,151,672 + -3.0 1,151,672 + -0.2 1,151,672 + -2.0 1,151,672 + +0.0 1,151,672 + -0.8 1,151,672 + +0.1 1,151,672 + -0.1 1,151,672 + -0.7 1,151,672 + -1.7 1,151,672 + -0.9 1,151,672 + -2.6 1,151,673 - +3.5 1,151,673 - -1.0 1,151,673 - -0.3 1,151,673 - +2.0 1,151,673 - +3.5 1,151,673 - +0.8 1,151,673 - -0.9 1,151,673 - -1.9 1,151,783 + Psest_1062 0.17 -3.0 1,152,053 + Psest_1062 0.59 +0.5 1,152,053 + Psest_1062 0.59 +2.1 1,152,054 - Psest_1062 0.59 +2.2 1,152,054 - Psest_1062 0.59 -1.2 1,152,054 - Psest_1062 0.59 -0.4 1,152,054 - Psest_1062 0.59 -2.7 1,152,054 - Psest_1062 0.59 -1.5 1,152,057 - Psest_1062 0.59 -0.2 1,152,218 + Psest_1062 0.84 -0.3 1,152,219 - Psest_1062 0.84 +0.6 1,152,219 - Psest_1062 0.84 +0.1 1,152,219 - Psest_1062 0.84 -0.7 1,152,219 - Psest_1062 0.84 +4.0 1,152,406 + +1.7 1,152,406 + -0.4 1,152,406 + -1.6 1,152,406 + -1.9 1,152,406 + +0.2 1,152,406 + -0.9 1,152,406 + +0.5 1,152,407 - -0.1 1,152,407 - +2.0 1,152,407 - -1.4 1,152,407 - +0.7 1,152,407 - +0.3 1,152,407 - +0.1 1,152,407 - -3.1 1,152,407 - +0.8 1,152,565 + Psest_1063 0.27 -1.8 1,152,565 + Psest_1063 0.27 +1.4 1,152,565 + Psest_1063 0.27 -1.9 1,152,566 - Psest_1063 0.27 +1.8 1,152,566 - Psest_1063 0.27 -1.0 1,152,566 - Psest_1063 0.27 -0.9 1,152,580 + Psest_1063 0.29 -3.0 1,152,581 - Psest_1063 0.29 +0.8 1,152,581 - Psest_1063 0.29 -0.6
Or see this region's nucleotide sequence