Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SOA0167 and SOA0168 are separated by 163 nucleotides SOA0168 and SOA0169 overlap by 4 nucleotides SOA0169 and SOA0170 overlap by 7 nucleotides SOA0170 and SOA0171 are separated by 272 nucleotides
SOA0167: SOA0167 - ISSod9, transposase (NCBI ptt file), at 155,302 to 158,268
SOA0167
SOA0168: SOA0168 - ISSo9, nucleotidyltransferase domain protein (NCBI ptt file), at 158,432 to 158,722
SOA0168
SOA0169: SOA0169 - ISSod9, conserved hypothetical protein (NCBI ptt file), at 158,719 to 159,096
SOA0169
SOA0170: SOA0170 - ISSod9, DNA-invertase (NCBI ptt file), at 159,090 to 159,647
SOA0170
SOA0171: SOA0171 - hypothetical protein (NCBI ptt file), at 159,920 to 160,177
SOA0171
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1 at 159.687 kb on + strand at 159.699 kb on + strand at 159.700 kb on - strand at 159.700 kb on - strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.709 kb on + strand at 159.778 kb on + strand at 159.778 kb on - strand at 159.783 kb on + strand at 159.791 kb on - strand at 159.791 kb on - strand at 159.813 kb on - strand at 159.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 159,687 + -1.7 159,699 + -2.7 159,700 - -2.3 159,700 - -1.6 159,709 + -0.3 159,709 + -2.0 159,709 + -1.8 159,709 + -1.4 159,778 + -0.4 159,778 - -0.7 159,783 + -0.4 159,791 - -2.2 159,791 - -1.8 159,813 - +0.6 159,840 - -2.4
Or see this region's nucleotide sequence