Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4374 and SO4375 overlap by 4 nucleotides SO4375 and SO4376 overlap by 4 nucleotides SO4376 and SO4377 overlap by 1 nucleotides
SO4374: SO4374 - histidine ammonia-lyase, putative (NCBI ptt file), at 4,567,930 to 4,569,495
SO4374
SO4375: SO4375 - hypothetical 4-hydroxybenzoyl-CoA thioesterase (NCBI ptt file), at 4,569,492 to 4,569,926
SO4375
SO4376: SO4376 - hypothetical protein (NCBI ptt file), at 4,569,923 to 4,570,753
SO4376
SO4377: SO4377 - membrane protein, putative (NCBI ptt file), at 4,570,753 to 4,573,257
SO4377
Position (kb)
4569
4570 Strain fitness (log2 ratio)
-2
-1
0
1 at 4568.495 kb on - strand, within SO4374 at 4568.536 kb on + strand, within SO4374 at 4568.545 kb on - strand, within SO4374 at 4568.563 kb on - strand, within SO4374 at 4568.587 kb on + strand, within SO4374 at 4568.590 kb on - strand, within SO4374 at 4568.643 kb on + strand, within SO4374 at 4568.651 kb on + strand, within SO4374 at 4568.671 kb on - strand, within SO4374 at 4568.671 kb on - strand, within SO4374 at 4568.700 kb on + strand, within SO4374 at 4568.764 kb on - strand, within SO4374 at 4568.965 kb on + strand, within SO4374 at 4569.029 kb on - strand, within SO4374 at 4569.093 kb on - strand, within SO4374 at 4569.110 kb on + strand, within SO4374 at 4569.110 kb on + strand, within SO4374 at 4569.110 kb on + strand, within SO4374 at 4569.118 kb on - strand, within SO4374 at 4569.332 kb on + strand, within SO4374 at 4569.362 kb on + strand at 4569.415 kb on + strand at 4569.445 kb on + strand at 4569.484 kb on - strand at 4569.535 kb on + strand at 4569.571 kb on + strand, within SO4375 at 4569.624 kb on + strand, within SO4375 at 4569.698 kb on + strand, within SO4375 at 4569.714 kb on - strand, within SO4375 at 4569.716 kb on + strand, within SO4375 at 4569.716 kb on + strand, within SO4375 at 4569.803 kb on - strand, within SO4375 at 4569.812 kb on + strand, within SO4375 at 4569.820 kb on - strand, within SO4375 at 4569.868 kb on + strand, within SO4375 at 4569.913 kb on - strand at 4569.986 kb on + strand at 4569.986 kb on + strand at 4569.994 kb on - strand at 4569.994 kb on - strand at 4569.994 kb on - strand at 4569.994 kb on - strand at 4569.994 kb on - strand at 4570.001 kb on + strand at 4570.004 kb on - strand at 4570.004 kb on - strand at 4570.004 kb on - strand at 4570.011 kb on + strand, within SO4376 at 4570.016 kb on + strand, within SO4376 at 4570.019 kb on - strand, within SO4376 at 4570.019 kb on - strand, within SO4376 at 4570.019 kb on - strand, within SO4376 at 4570.019 kb on - strand, within SO4376 at 4570.019 kb on - strand, within SO4376 at 4570.029 kb on + strand, within SO4376 at 4570.208 kb on + strand, within SO4376 at 4570.237 kb on - strand, within SO4376 at 4570.392 kb on + strand, within SO4376 at 4570.392 kb on - strand, within SO4376 at 4570.395 kb on - strand, within SO4376 at 4570.400 kb on - strand, within SO4376 at 4570.431 kb on + strand, within SO4376 at 4570.560 kb on - strand, within SO4376 at 4570.571 kb on + strand, within SO4376 at 4570.578 kb on + strand, within SO4376 at 4570.578 kb on + strand, within SO4376 at 4570.586 kb on - strand, within SO4376 at 4570.601 kb on - strand, within SO4376 at 4570.619 kb on + strand, within SO4376 at 4570.656 kb on - strand, within SO4376 at 4570.684 kb on + strand at 4570.887 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,568,495 - SO4374 0.36 -0.0 4,568,536 + SO4374 0.39 +0.2 4,568,545 - SO4374 0.39 +0.7 4,568,563 - SO4374 0.40 -0.5 4,568,587 + SO4374 0.42 -0.2 4,568,590 - SO4374 0.42 +0.1 4,568,643 + SO4374 0.46 +0.8 4,568,651 + SO4374 0.46 -0.3 4,568,671 - SO4374 0.47 +0.1 4,568,671 - SO4374 0.47 -0.5 4,568,700 + SO4374 0.49 -0.3 4,568,764 - SO4374 0.53 +0.1 4,568,965 + SO4374 0.66 -0.5 4,569,029 - SO4374 0.70 +0.2 4,569,093 - SO4374 0.74 +0.7 4,569,110 + SO4374 0.75 -0.3 4,569,110 + SO4374 0.75 +0.5 4,569,110 + SO4374 0.75 +1.1 4,569,118 - SO4374 0.76 -0.2 4,569,332 + SO4374 0.90 -0.3 4,569,362 + +0.4 4,569,415 + -0.3 4,569,445 + +0.9 4,569,484 - -0.3 4,569,535 + +0.7 4,569,571 + SO4375 0.18 +1.3 4,569,624 + SO4375 0.30 +0.3 4,569,698 + SO4375 0.47 -0.6 4,569,714 - SO4375 0.51 +0.3 4,569,716 + SO4375 0.51 +0.1 4,569,716 + SO4375 0.51 +0.1 4,569,803 - SO4375 0.71 +0.1 4,569,812 + SO4375 0.74 -0.1 4,569,820 - SO4375 0.75 -0.0 4,569,868 + SO4375 0.86 -0.9 4,569,913 - -0.9 4,569,986 + -0.3 4,569,986 + -0.0 4,569,994 - -0.3 4,569,994 - -0.5 4,569,994 - +0.0 4,569,994 - -0.4 4,569,994 - -0.6 4,570,001 + -1.9 4,570,004 - -0.7 4,570,004 - -0.3 4,570,004 - -0.7 4,570,011 + SO4376 0.11 -1.3 4,570,016 + SO4376 0.11 -0.8 4,570,019 - SO4376 0.12 -0.6 4,570,019 - SO4376 0.12 -0.9 4,570,019 - SO4376 0.12 -0.6 4,570,019 - SO4376 0.12 +0.0 4,570,019 - SO4376 0.12 -0.5 4,570,029 + SO4376 0.13 -0.7 4,570,208 + SO4376 0.34 -1.7 4,570,237 - SO4376 0.38 -0.4 4,570,392 + SO4376 0.56 -1.7 4,570,392 - SO4376 0.56 -0.4 4,570,395 - SO4376 0.57 -0.5 4,570,400 - SO4376 0.57 -0.3 4,570,431 + SO4376 0.61 -0.4 4,570,560 - SO4376 0.77 -0.9 4,570,571 + SO4376 0.78 -0.5 4,570,578 + SO4376 0.79 -0.4 4,570,578 + SO4376 0.79 -0.0 4,570,586 - SO4376 0.80 -0.1 4,570,601 - SO4376 0.82 +0.1 4,570,619 + SO4376 0.84 -1.5 4,570,656 - SO4376 0.88 -0.4 4,570,684 + -0.1 4,570,887 + -0.5
Or see this region's nucleotide sequence