Strain Fitness in Shewanella oneidensis MR-1 around SO3833

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO3832 and prfA are separated by 2 nucleotidesprfA and hemA are separated by 14 nucleotides SO3832: SO3832 - hemK family protein (NCBI ptt file), at 3,981,537 to 3,982,397 SO3832 SO3833: prfA - peptide chain release factor 1 (NCBI ptt file), at 3,982,400 to 3,983,491 prfA SO3834: hemA - glutamyl-tRNA reductase (NCBI ptt file), at 3,983,506 to 3,984,756 hemA Position (kb) 3982 3983 3984Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3981.415 kb on + strandat 3981.415 kb on + strandat 3981.446 kb on + strandat 3981.513 kb on - strandat 3981.530 kb on - strandat 3981.659 kb on + strand, within SO3832at 3981.703 kb on - strand, within SO3832at 3981.752 kb on - strand, within SO3832at 3981.918 kb on - strand, within SO3832at 3981.939 kb on - strand, within SO3832at 3982.054 kb on - strand, within SO3832at 3982.056 kb on + strand, within SO3832at 3982.232 kb on + strand, within SO3832at 3982.232 kb on + strand, within SO3832at 3982.247 kb on - strand, within SO3832

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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3,981,415 + +0.8
3,981,415 + +0.0
3,981,446 + +0.7
3,981,513 - -0.4
3,981,530 - +0.2
3,981,659 + SO3832 0.14 -2.7
3,981,703 - SO3832 0.19 -3.8
3,981,752 - SO3832 0.25 -2.7
3,981,918 - SO3832 0.44 -3.1
3,981,939 - SO3832 0.47 -2.8
3,982,054 - SO3832 0.60 -3.5
3,982,056 + SO3832 0.60 -3.1
3,982,232 + SO3832 0.81 -1.6
3,982,232 + SO3832 0.81 -2.7
3,982,247 - SO3832 0.82 -3.5

Or see this region's nucleotide sequence