Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proC and SO3355 are separated by 76 nucleotides SO3355 and SO3356 overlap by 1 nucleotides SO3356 and SO3357 are separated by 67 nucleotides SO3357 and SO3358 are separated by 117 nucleotides
SO3354: proC - pyrroline-5-carboxylate reductase (NCBI ptt file), at 3,498,002 to 3,498,820
proC
SO3355: SO3355 - conserved hypothetical protein (NCBI ptt file), at 3,498,897 to 3,499,445
SO3355
SO3356: SO3356 - conserved hypothetical protein TIGR00251 (NCBI ptt file), at 3,499,445 to 3,499,735
SO3356
SO3357: SO3357 - conserved hypothetical protein (NCBI ptt file), at 3,499,803 to 3,500,237
SO3357
SO3358: SO3358 - HAM1 protein (NCBI ptt file), at 3,500,355 to 3,500,972
SO3358
Position (kb)
3498
3499
3500 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3497.954 kb on + strand at 3497.974 kb on + strand at 3497.974 kb on + strand at 3497.974 kb on + strand at 3497.974 kb on + strand at 3497.982 kb on - strand at 3498.034 kb on + strand at 3498.063 kb on + strand at 3498.094 kb on + strand, within proC at 3498.108 kb on + strand, within proC at 3498.108 kb on + strand, within proC at 3498.116 kb on - strand, within proC at 3498.116 kb on - strand, within proC at 3498.178 kb on - strand, within proC at 3498.180 kb on + strand, within proC at 3498.210 kb on + strand, within proC at 3498.238 kb on - strand, within proC at 3498.255 kb on + strand, within proC at 3498.324 kb on + strand, within proC at 3498.346 kb on + strand, within proC at 3498.374 kb on - strand, within proC at 3498.457 kb on - strand, within proC at 3498.590 kb on + strand, within proC at 3498.696 kb on + strand, within proC at 3498.696 kb on + strand, within proC at 3498.704 kb on - strand, within proC at 3498.711 kb on - strand, within proC at 3498.716 kb on + strand, within proC at 3498.920 kb on - strand at 3498.972 kb on + strand, within SO3355 at 3499.044 kb on + strand, within SO3355 at 3499.044 kb on + strand, within SO3355 at 3499.054 kb on + strand, within SO3355 at 3499.139 kb on - strand, within SO3355 at 3499.266 kb on - strand, within SO3355 at 3499.323 kb on + strand, within SO3355 at 3499.447 kb on - strand at 3499.447 kb on - strand at 3499.542 kb on + strand, within SO3356 at 3499.693 kb on + strand, within SO3356 at 3499.701 kb on - strand, within SO3356 at 3499.758 kb on + strand at 3499.845 kb on - strand at 3499.908 kb on + strand, within SO3357 at 3499.916 kb on - strand, within SO3357 at 3499.946 kb on + strand, within SO3357 at 3499.954 kb on + strand, within SO3357 at 3499.962 kb on - strand, within SO3357 at 3500.013 kb on - strand, within SO3357 at 3500.123 kb on - strand, within SO3357 at 3500.208 kb on - strand at 3500.428 kb on + strand, within SO3358 at 3500.436 kb on - strand, within SO3358
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,497,954 + -0.3 3,497,974 + +0.5 3,497,974 + +0.2 3,497,974 + +0.1 3,497,974 + -0.4 3,497,982 - -1.1 3,498,034 + +0.2 3,498,063 + +0.1 3,498,094 + proC SO3354 0.11 +0.2 3,498,108 + proC SO3354 0.13 -0.5 3,498,108 + proC SO3354 0.13 -0.2 3,498,116 - proC SO3354 0.14 -0.6 3,498,116 - proC SO3354 0.14 -1.0 3,498,178 - proC SO3354 0.21 -0.8 3,498,180 + proC SO3354 0.22 -0.2 3,498,210 + proC SO3354 0.25 -0.5 3,498,238 - proC SO3354 0.29 -1.3 3,498,255 + proC SO3354 0.31 -1.2 3,498,324 + proC SO3354 0.39 +0.0 3,498,346 + proC SO3354 0.42 -0.1 3,498,374 - proC SO3354 0.45 -1.4 3,498,457 - proC SO3354 0.56 -1.3 3,498,590 + proC SO3354 0.72 +0.7 3,498,696 + proC SO3354 0.85 -0.5 3,498,696 + proC SO3354 0.85 -0.4 3,498,704 - proC SO3354 0.86 -2.1 3,498,711 - proC SO3354 0.87 -3.8 3,498,716 + proC SO3354 0.87 +0.6 3,498,920 - -2.3 3,498,972 + SO3355 0.14 -2.9 3,499,044 + SO3355 0.27 -3.7 3,499,044 + SO3355 0.27 -3.5 3,499,054 + SO3355 0.29 -2.7 3,499,139 - SO3355 0.44 -4.5 3,499,266 - SO3355 0.67 -3.3 3,499,323 + SO3355 0.78 -4.2 3,499,447 - +0.1 3,499,447 - +0.4 3,499,542 + SO3356 0.33 +0.3 3,499,693 + SO3356 0.85 -0.4 3,499,701 - SO3356 0.88 +0.3 3,499,758 + +0.4 3,499,845 - +0.2 3,499,908 + SO3357 0.24 -0.7 3,499,916 - SO3357 0.26 -0.2 3,499,946 + SO3357 0.33 +0.6 3,499,954 + SO3357 0.35 +0.1 3,499,962 - SO3357 0.37 -0.0 3,500,013 - SO3357 0.48 -0.3 3,500,123 - SO3357 0.74 -0.6 3,500,208 - +0.1 3,500,428 + SO3358 0.12 +0.1 3,500,436 - SO3358 0.13 +0.6
Or see this region's nucleotide sequence