Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO_2233 and SO2234 are separated by 430 nucleotides SO2234 and crr are separated by 145 nucleotides crr and ptsI are separated by 71 nucleotides
SO_2233: SO_2233 - no description, at 2,351,057 to 2,351,716
_2233
SO2234: SO2234 - hypothetical protein (NCBI ptt file), at 2,352,147 to 2,352,518
SO2234
SO2236: crr - PTS system, glucose-specific IIA component (NCBI ptt file), at 2,352,664 to 2,353,173
crr
SO2237: ptsI - phosphoenolpyruvate-protein phosphotransferase (NCBI ptt file), at 2,353,245 to 2,354,948
ptsI
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-1
0
1
2 at 2351.696 kb on + strand at 2351.916 kb on + strand at 2352.071 kb on - strand at 2352.218 kb on - strand, within SO2234 at 2352.316 kb on + strand, within SO2234 at 2352.339 kb on + strand, within SO2234 at 2352.452 kb on - strand, within SO2234 at 2352.521 kb on + strand at 2352.662 kb on - strand at 2352.717 kb on - strand, within crr at 2352.872 kb on + strand, within crr at 2352.879 kb on + strand, within crr at 2352.879 kb on + strand, within crr at 2352.879 kb on + strand, within crr at 2352.912 kb on + strand, within crr at 2352.912 kb on + strand, within crr at 2352.993 kb on + strand, within crr at 2353.143 kb on - strand at 2353.216 kb on + strand at 2353.258 kb on + strand at 2353.376 kb on + strand at 2353.384 kb on - strand at 2353.800 kb on + strand, within ptsI at 2354.095 kb on + strand, within ptsI
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,351,696 + +0.8 2,351,916 + +1.8 2,352,071 - +2.1 2,352,218 - SO2234 0.19 +2.4 2,352,316 + SO2234 0.45 +1.6 2,352,339 + SO2234 0.52 +0.2 2,352,452 - SO2234 0.82 +2.5 2,352,521 + +0.5 2,352,662 - +1.6 2,352,717 - crr SO2236 0.10 +1.4 2,352,872 + crr SO2236 0.41 +1.2 2,352,879 + crr SO2236 0.42 -0.3 2,352,879 + crr SO2236 0.42 +1.2 2,352,879 + crr SO2236 0.42 +0.6 2,352,912 + crr SO2236 0.49 +1.5 2,352,912 + crr SO2236 0.49 +1.3 2,352,993 + crr SO2236 0.65 +1.1 2,353,143 - +2.5 2,353,216 + +1.5 2,353,258 + +2.2 2,353,376 + -1.4 2,353,384 - +1.1 2,353,800 + ptsI SO2237 0.33 +1.1 2,354,095 + ptsI SO2237 0.50 +2.3
Or see this region's nucleotide sequence