Strain Fitness in Shewanella oneidensis MR-1 around SO1809

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpspA and pspB are separated by 17 nucleotidespspB and pspC overlap by 14 nucleotidespspC and SO1810 are separated by 114 nucleotides SO1807: pspA - phage shock protein A (NCBI ptt file), at 1,897,828 to 1,898,511 pspA SO1808: pspB - phage shock protein B (NCBI ptt file), at 1,898,529 to 1,898,768 pspB SO1809: pspC - phage shock protein C (NCBI ptt file), at 1,898,755 to 1,899,141 pspC SO1810: SO1810 - hypothetical ATPase (NCBI ptt file), at 1,899,256 to 1,900,713 SO1810 Position (kb) 1898 1899 1900Strain fitness (log2 ratio) -3 -2 -1 0 1at 1897.857 kb on + strandat 1897.863 kb on + strandat 1897.891 kb on + strandat 1897.947 kb on + strand, within pspAat 1898.142 kb on - strand, within pspAat 1898.253 kb on + strand, within pspAat 1898.512 kb on + strandat 1898.707 kb on - strand, within pspBat 1898.830 kb on + strand, within pspCat 1898.830 kb on + strand, within pspCat 1898.838 kb on - strand, within pspCat 1898.941 kb on - strand, within pspCat 1898.961 kb on - strand, within pspCat 1898.999 kb on - strand, within pspCat 1899.059 kb on - strand, within pspCat 1899.481 kb on - strand, within SO1810at 1899.497 kb on - strand, within SO1810at 1899.587 kb on - strand, within SO1810at 1899.589 kb on + strand, within SO1810at 1899.756 kb on - strand, within SO1810at 1900.005 kb on + strand, within SO1810at 1900.034 kb on + strand, within SO1810at 1900.042 kb on - strand, within SO1810at 1900.042 kb on - strand, within SO1810

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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1,897,857 + +0.0
1,897,863 + -0.8
1,897,891 + -0.5
1,897,947 + pspA SO1807 0.17 -2.1
1,898,142 - pspA SO1807 0.46 -0.9
1,898,253 + pspA SO1807 0.62 -0.4
1,898,512 + -0.5
1,898,707 - pspB SO1808 0.74 -1.7
1,898,830 + pspC SO1809 0.19 -1.5
1,898,830 + pspC SO1809 0.19 -0.1
1,898,838 - pspC SO1809 0.21 -1.4
1,898,941 - pspC SO1809 0.48 -2.9
1,898,961 - pspC SO1809 0.53 -1.5
1,898,999 - pspC SO1809 0.63 -3.2
1,899,059 - pspC SO1809 0.79 -1.5
1,899,481 - SO1810 0.15 -0.6
1,899,497 - SO1810 0.17 -0.1
1,899,587 - SO1810 0.23 +0.3
1,899,589 + SO1810 0.23 -0.2
1,899,756 - SO1810 0.34 -0.3
1,900,005 + SO1810 0.51 -0.6
1,900,034 + SO1810 0.53 +0.7
1,900,042 - SO1810 0.54 -0.5
1,900,042 - SO1810 0.54 +0.8

Or see this region's nucleotide sequence