Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0666 and Psest_0667 are separated by 147 nucleotides Psest_0667 and Psest_0668 are separated by 6 nucleotides Psest_0668 and Psest_0669 are separated by 85 nucleotides
Psest_0666: Psest_0666 - Uncharacterized protein conserved in bacteria, at 725,370 to 726,182
_0666
Psest_0667: Psest_0667 - Phosphodiesterase/alkaline phosphatase D, at 726,330 to 727,883
_0667
Psest_0668: Psest_0668 - Ethanolamine ammonia-lyase, small subunit, at 727,890 to 728,684
_0668
Psest_0669: Psest_0669 - Ethanolamine ammonia-lyase, large subunit, at 728,770 to 730,161
_0669
Position (kb)
726
727
728 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 725.371 kb on + strand at 725.372 kb on - strand at 725.461 kb on + strand, within Psest_0666 at 725.462 kb on - strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.510 kb on - strand, within Psest_0666 at 725.614 kb on + strand, within Psest_0666 at 725.737 kb on + strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.034 kb on - strand, within Psest_0666 at 726.034 kb on - strand, within Psest_0666 at 726.216 kb on + strand at 726.216 kb on + strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.250 kb on + strand at 726.251 kb on - strand at 726.293 kb on - strand at 726.822 kb on + strand, within Psest_0667 at 726.822 kb on + strand, within Psest_0667 at 726.822 kb on + strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.996 kb on + strand, within Psest_0667 at 726.996 kb on + strand, within Psest_0667 at 726.997 kb on - strand, within Psest_0667 at 727.002 kb on + strand, within Psest_0667 at 727.224 kb on + strand, within Psest_0667 at 727.225 kb on - strand, within Psest_0667 at 727.239 kb on + strand, within Psest_0667 at 727.239 kb on + strand, within Psest_0667 at 727.302 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.579 kb on - strand, within Psest_0667 at 727.593 kb on + strand, within Psest_0667 at 727.594 kb on - strand, within Psest_0667 at 727.594 kb on - strand, within Psest_0667 at 727.596 kb on + strand, within Psest_0667 at 727.596 kb on + strand, within Psest_0667 at 727.597 kb on - strand, within Psest_0667 at 727.597 kb on - strand, within Psest_0667 at 727.597 kb on - strand, within Psest_0667 at 727.617 kb on + strand, within Psest_0667 at 727.617 kb on + strand, within Psest_0667 at 727.817 kb on - strand at 727.817 kb on - strand at 727.849 kb on - strand at 727.938 kb on + strand at 727.962 kb on + strand at 728.002 kb on + strand, within Psest_0668 at 728.002 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.030 kb on - strand, within Psest_0668 at 728.030 kb on - strand, within Psest_0668 at 728.030 kb on - strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.435 kb on - strand at 728.483 kb on + strand, within Psest_0668 at 728.483 kb on + strand, within Psest_0668 at 728.483 kb on + strand, within Psest_0668 at 728.484 kb on - strand, within Psest_0668 at 728.484 kb on - strand, within Psest_0668 at 728.688 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 725,371 + +1.3 725,372 - -0.5 725,461 + Psest_0666 0.11 -0.5 725,462 - Psest_0666 0.11 +0.2 725,475 + Psest_0666 0.13 +0.2 725,475 + Psest_0666 0.13 +0.6 725,476 - Psest_0666 0.13 -0.3 725,476 - Psest_0666 0.13 +0.2 725,509 + Psest_0666 0.17 +0.1 725,509 + Psest_0666 0.17 +0.7 725,510 - Psest_0666 0.17 -1.1 725,614 + Psest_0666 0.30 +1.1 725,737 + Psest_0666 0.45 +0.8 725,738 - Psest_0666 0.45 -0.9 725,738 - Psest_0666 0.45 -0.7 725,738 - Psest_0666 0.45 -0.4 725,738 - Psest_0666 0.45 -1.5 725,738 - Psest_0666 0.45 -0.3 725,738 - Psest_0666 0.45 +0.5 725,738 - Psest_0666 0.45 -0.9 726,033 + Psest_0666 0.82 +1.1 726,033 + Psest_0666 0.82 +0.1 726,033 + Psest_0666 0.82 +0.7 726,033 + Psest_0666 0.82 +0.1 726,033 + Psest_0666 0.82 +0.1 726,034 - Psest_0666 0.82 +0.5 726,034 - Psest_0666 0.82 -1.4 726,216 + -0.5 726,216 + +1.9 726,217 - -0.5 726,217 - -1.7 726,217 - -2.1 726,217 - +1.9 726,217 - -0.2 726,250 + +0.9 726,251 - -2.7 726,293 - +0.2 726,822 + Psest_0667 0.32 +0.3 726,822 + Psest_0667 0.32 +1.5 726,822 + Psest_0667 0.32 +0.8 726,823 - Psest_0667 0.32 +0.2 726,823 - Psest_0667 0.32 +1.5 726,823 - Psest_0667 0.32 +0.9 726,823 - Psest_0667 0.32 +2.7 726,879 + Psest_0667 0.35 +1.1 726,879 + Psest_0667 0.35 -1.5 726,879 + Psest_0667 0.35 +1.6 726,879 + Psest_0667 0.35 +1.1 726,879 + Psest_0667 0.35 -0.5 726,879 + Psest_0667 0.35 -0.5 726,879 + Psest_0667 0.35 +3.5 726,879 + Psest_0667 0.35 +1.7 726,880 - Psest_0667 0.35 +1.1 726,880 - Psest_0667 0.35 +0.7 726,880 - Psest_0667 0.35 +0.2 726,996 + Psest_0667 0.43 +1.1 726,996 + Psest_0667 0.43 -1.1 726,997 - Psest_0667 0.43 +1.5 727,002 + Psest_0667 0.43 +0.1 727,224 + Psest_0667 0.58 +2.1 727,225 - Psest_0667 0.58 -0.1 727,239 + Psest_0667 0.58 +0.9 727,239 + Psest_0667 0.58 -0.7 727,302 - Psest_0667 0.63 +2.1 727,334 - Psest_0667 0.65 +0.4 727,334 - Psest_0667 0.65 +2.4 727,334 - Psest_0667 0.65 +1.1 727,334 - Psest_0667 0.65 -2.2 727,334 - Psest_0667 0.65 -0.3 727,579 - Psest_0667 0.80 -2.1 727,593 + Psest_0667 0.81 +0.3 727,594 - Psest_0667 0.81 +0.3 727,594 - Psest_0667 0.81 -1.2 727,596 + Psest_0667 0.81 -0.9 727,596 + Psest_0667 0.81 +0.6 727,597 - Psest_0667 0.82 -1.7 727,597 - Psest_0667 0.82 -0.1 727,597 - Psest_0667 0.82 -0.5 727,617 + Psest_0667 0.83 -0.9 727,617 + Psest_0667 0.83 +0.1 727,817 - -0.1 727,817 - -0.4 727,849 - +0.1 727,938 + -0.1 727,962 + -1.2 728,002 + Psest_0668 0.14 +0.3 728,002 + Psest_0668 0.14 -0.0 728,029 + Psest_0668 0.17 +0.9 728,029 + Psest_0668 0.17 -1.2 728,029 + Psest_0668 0.17 +3.4 728,029 + Psest_0668 0.17 -1.0 728,029 + Psest_0668 0.17 -0.1 728,029 + Psest_0668 0.17 +0.5 728,029 + Psest_0668 0.17 -0.2 728,030 - Psest_0668 0.18 -0.9 728,030 - Psest_0668 0.18 -0.2 728,030 - Psest_0668 0.18 +0.1 728,110 + Psest_0668 0.28 -0.2 728,110 + Psest_0668 0.28 +1.1 728,110 + Psest_0668 0.28 -1.0 728,110 + Psest_0668 0.28 -0.7 728,111 - Psest_0668 0.28 +0.2 728,111 - Psest_0668 0.28 -1.4 728,111 - Psest_0668 0.28 -0.5 728,111 - Psest_0668 0.28 -0.7 728,111 - Psest_0668 0.28 +1.2 728,434 + Psest_0668 0.68 +0.1 728,434 + Psest_0668 0.68 +0.6 728,434 + Psest_0668 0.68 -0.4 728,434 + Psest_0668 0.68 +0.1 728,435 - +0.5 728,483 + Psest_0668 0.75 +1.2 728,483 + Psest_0668 0.75 +0.5 728,483 + Psest_0668 0.75 -0.7 728,484 - Psest_0668 0.75 -0.2 728,484 - Psest_0668 0.75 +0.1 728,688 + -0.5
Or see this region's nucleotide sequence