Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2582 and Psest_2583 overlap by 4 nucleotides Psest_2583 and Psest_2584 are separated by 210 nucleotides Psest_2584 and Psest_2585 are separated by 91 nucleotides
Psest_2582: Psest_2582 - folylpolyglutamate synthase/dihydrofolate synthase, at 2,762,038 to 2,763,342
_2582
Psest_2583: Psest_2583 - acetyl-CoA carboxylase, carboxyl transferase, beta subunit, at 2,763,339 to 2,764,232
_2583
Psest_2584: Psest_2584 - Phosphoribosylanthranilate isomerase, at 2,764,443 to 2,765,066
_2584
Psest_2585: Psest_2585 - pseudouridylate synthase I, at 2,765,158 to 2,766,018
_2585
Position (kb)
2763
2764
2765 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2764.305 kb on - strand at 2764.305 kb on - strand at 2764.305 kb on - strand at 2764.390 kb on - strand at 2764.683 kb on - strand, within Psest_2584 at 2764.695 kb on - strand, within Psest_2584 at 2764.720 kb on - strand, within Psest_2584 at 2764.896 kb on - strand, within Psest_2584 at 2764.899 kb on - strand, within Psest_2584 at 2764.977 kb on + strand, within Psest_2584 at 2764.977 kb on + strand, within Psest_2584 at 2764.978 kb on - strand, within Psest_2584 at 2764.978 kb on - strand, within Psest_2584 at 2764.978 kb on - strand, within Psest_2584 at 2765.043 kb on + strand at 2765.044 kb on - strand at 2765.071 kb on + strand at 2765.071 kb on + strand at 2765.075 kb on + strand at 2765.075 kb on + strand at 2765.075 kb on + strand at 2765.075 kb on + strand at 2765.076 kb on - strand at 2765.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 2,764,305 - +1.3 2,764,305 - -0.3 2,764,305 - -0.3 2,764,390 - +0.2 2,764,683 - Psest_2584 0.38 -3.3 2,764,695 - Psest_2584 0.40 -4.1 2,764,720 - Psest_2584 0.44 -2.3 2,764,896 - Psest_2584 0.73 -2.4 2,764,899 - Psest_2584 0.73 -4.2 2,764,977 + Psest_2584 0.86 -1.9 2,764,977 + Psest_2584 0.86 -2.2 2,764,978 - Psest_2584 0.86 -2.9 2,764,978 - Psest_2584 0.86 -5.2 2,764,978 - Psest_2584 0.86 -2.0 2,765,043 + -3.0 2,765,044 - -1.0 2,765,071 + -3.8 2,765,071 + -3.2 2,765,075 + -1.7 2,765,075 + -3.2 2,765,075 + -2.9 2,765,075 + -5.2 2,765,076 - -1.8 2,765,080 - -0.9
Or see this region's nucleotide sequence