Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0247 and Psest_0248 are separated by 129 nucleotides Psest_0248 and Psest_0249 are separated by 175 nucleotides Psest_0249 and Psest_0250 overlap by 4 nucleotides
Psest_0247: Psest_0247 - NAD-dependent aldehyde dehydrogenases, at 257,716 to 259,128
_0247
Psest_0248: Psest_0248 - Transcriptional regulator, at 259,258 to 259,902
_0248
Psest_0249: Psest_0249 - UDP-N-acetylglucosamine 2-epimerase, at 260,078 to 261,268
_0249
Psest_0250: Psest_0250 - Glycosyltransferases, probably involved in cell wall biogenesis, at 261,265 to 263,451
_0250
Position (kb) 260
261
262 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 259.163 kb on - strand at 259.198 kb on + strand at 259.198 kb on + strand at 259.198 kb on + strand at 259.198 kb on + strand at 259.216 kb on + strand at 259.216 kb on + strand at 259.217 kb on - strand at 259.217 kb on - strand at 259.387 kb on + strand, within Psest_0248 at 259.387 kb on + strand, within Psest_0248 at 259.387 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.391 kb on - strand, within Psest_0248 at 259.438 kb on + strand, within Psest_0248 at 259.510 kb on + strand, within Psest_0248 at 259.510 kb on + strand, within Psest_0248 at 259.511 kb on - strand, within Psest_0248 at 259.511 kb on - strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.652 kb on - strand, within Psest_0248 at 259.715 kb on + strand, within Psest_0248 at 259.715 kb on + strand, within Psest_0248 at 259.715 kb on + strand, within Psest_0248 at 259.716 kb on - strand, within Psest_0248 at 259.807 kb on + strand, within Psest_0248 at 259.807 kb on + strand, within Psest_0248 at 259.807 kb on + strand, within Psest_0248 at 259.808 kb on - strand, within Psest_0248 at 259.862 kb on + strand at 259.863 kb on - strand at 259.863 kb on - strand at 259.863 kb on - strand at 259.863 kb on - strand at 259.868 kb on + strand at 259.869 kb on - strand at 259.869 kb on - strand at 259.869 kb on - strand at 259.936 kb on - strand at 260.105 kb on - strand at 260.173 kb on + strand at 260.356 kb on - strand, within Psest_0249 at 260.376 kb on + strand, within Psest_0249 at 260.376 kb on + strand, within Psest_0249 at 260.376 kb on + strand, within Psest_0249 at 260.377 kb on - strand, within Psest_0249 at 260.377 kb on - strand, within Psest_0249 at 260.472 kb on + strand, within Psest_0249 at 260.510 kb on + strand, within Psest_0249 at 260.677 kb on + strand, within Psest_0249 at 260.677 kb on + strand, within Psest_0249 at 260.677 kb on + strand, within Psest_0249 at 260.678 kb on - strand, within Psest_0249 at 260.678 kb on - strand, within Psest_0249 at 260.862 kb on + strand, within Psest_0249 at 260.862 kb on + strand, within Psest_0249 at 260.863 kb on - strand, within Psest_0249 at 260.894 kb on + strand, within Psest_0249 at 260.960 kb on + strand, within Psest_0249 at 261.045 kb on + strand, within Psest_0249 at 261.106 kb on + strand, within Psest_0249 at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.248 kb on - strand at 261.277 kb on + strand at 261.277 kb on + strand at 261.277 kb on + strand at 261.278 kb on - strand at 261.278 kb on - strand at 261.278 kb on - strand at 261.283 kb on + strand at 261.283 kb on + strand at 261.283 kb on + strand at 261.284 kb on - strand at 261.284 kb on - strand at 261.284 kb on - strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.310 kb on + strand at 261.310 kb on + strand at 261.311 kb on - strand at 261.311 kb on - strand at 261.311 kb on - strand at 261.311 kb on - strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.469 kb on + strand at 261.469 kb on + strand at 261.470 kb on - strand at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.528 kb on - strand, within Psest_0250 at 261.528 kb on - strand, within Psest_0250 at 261.528 kb on - strand, within Psest_0250 at 261.568 kb on + strand, within Psest_0250 at 261.568 kb on + strand, within Psest_0250 at 261.569 kb on - strand, within Psest_0250 at 261.578 kb on - strand, within Psest_0250 at 261.584 kb on + strand, within Psest_0250 at 261.584 kb on + strand, within Psest_0250 at 261.585 kb on - strand, within Psest_0250 at 261.594 kb on + strand, within Psest_0250 at 261.594 kb on + strand, within Psest_0250 at 261.594 kb on + strand, within Psest_0250 at 261.595 kb on - strand, within Psest_0250 at 261.595 kb on - strand, within Psest_0250 at 261.595 kb on - strand, within Psest_0250 at 261.829 kb on + strand, within Psest_0250 at 261.829 kb on + strand, within Psest_0250 at 261.829 kb on + strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.836 kb on + strand, within Psest_0250 at 261.869 kb on - strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 262.061 kb on + strand, within Psest_0250 at 262.061 kb on + strand, within Psest_0250 at 262.082 kb on - strand, within Psest_0250 at 262.082 kb on - strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.208 kb on + strand, within Psest_0250 at 262.218 kb on - strand, within Psest_0250
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 259,163 - +0.3 259,198 + +0.7 259,198 + -0.1 259,198 + +1.4 259,198 + -0.5 259,216 + +0.1 259,216 + -2.7 259,217 - -1.1 259,217 - -0.9 259,387 + Psest_0248 0.20 -0.0 259,387 + Psest_0248 0.20 +0.4 259,387 + Psest_0248 0.20 +0.4 259,390 + Psest_0248 0.20 -0.1 259,390 + Psest_0248 0.20 -1.1 259,390 + Psest_0248 0.20 -0.1 259,390 + Psest_0248 0.20 +0.1 259,390 + Psest_0248 0.20 -1.2 259,391 - Psest_0248 0.21 +0.3 259,438 + Psest_0248 0.28 -2.2 259,510 + Psest_0248 0.39 -0.5 259,510 + Psest_0248 0.39 +0.1 259,511 - Psest_0248 0.39 -0.9 259,511 - Psest_0248 0.39 -2.1 259,651 + Psest_0248 0.61 -1.9 259,651 + Psest_0248 0.61 -2.0 259,651 + Psest_0248 0.61 -0.5 259,651 + Psest_0248 0.61 -0.6 259,651 + Psest_0248 0.61 -2.9 259,652 - Psest_0248 0.61 -0.8 259,715 + Psest_0248 0.71 -1.5 259,715 + Psest_0248 0.71 -1.5 259,715 + Psest_0248 0.71 -0.6 259,716 - Psest_0248 0.71 -1.4 259,807 + Psest_0248 0.85 -2.3 259,807 + Psest_0248 0.85 +0.2 259,807 + Psest_0248 0.85 +1.3 259,808 - Psest_0248 0.85 -2.1 259,862 + -0.2 259,863 - -0.2 259,863 - -2.6 259,863 - +0.7 259,863 - -1.0 259,868 + +0.3 259,869 - -1.2 259,869 - -0.8 259,869 - -0.8 259,936 - -0.6 260,105 - +0.7 260,173 + -1.9 260,356 - Psest_0249 0.23 -0.1 260,376 + Psest_0249 0.25 -2.0 260,376 + Psest_0249 0.25 -0.4 260,376 + Psest_0249 0.25 +1.6 260,377 - Psest_0249 0.25 -0.7 260,377 - Psest_0249 0.25 -0.3 260,472 + Psest_0249 0.33 +0.9 260,510 + Psest_0249 0.36 -0.6 260,677 + Psest_0249 0.50 +0.6 260,677 + Psest_0249 0.50 -2.3 260,677 + Psest_0249 0.50 -1.3 260,678 - Psest_0249 0.50 +0.1 260,678 - Psest_0249 0.50 -3.1 260,862 + Psest_0249 0.66 -2.1 260,862 + Psest_0249 0.66 -1.3 260,863 - Psest_0249 0.66 -0.3 260,894 + Psest_0249 0.69 +0.6 260,960 + Psest_0249 0.74 -3.2 261,045 + Psest_0249 0.81 -0.6 261,106 + Psest_0249 0.86 -2.8 261,220 + -1.5 261,220 + -1.8 261,220 + +0.8 261,220 + -2.0 261,220 + -0.8 261,220 + -1.7 261,221 - +0.9 261,221 - -0.8 261,221 - +0.3 261,221 - -0.3 261,221 - +0.6 261,221 - -0.2 261,221 - -0.6 261,221 - -0.0 261,221 - -0.7 261,221 - +1.5 261,221 - -0.2 261,248 - -0.8 261,277 + -2.2 261,277 + -1.4 261,277 + +0.7 261,278 - -0.4 261,278 - -0.0 261,278 - +0.4 261,283 + -0.4 261,283 + +0.9 261,283 + -0.7 261,284 - -0.4 261,284 - -2.2 261,284 - +0.0 261,307 + -0.2 261,307 + +0.2 261,307 + -2.4 261,307 + +0.1 261,307 + +0.2 261,308 - +1.1 261,308 - +1.7 261,308 - +0.3 261,308 - +1.0 261,308 - -1.4 261,310 + -2.1 261,310 + -1.1 261,311 - -0.3 261,311 - -0.3 261,311 - -0.1 261,311 - -0.9 261,316 + -1.2 261,316 + -1.9 261,316 + -0.6 261,316 + +0.2 261,316 + -2.0 261,316 + -1.8 261,317 - -0.1 261,317 - -1.3 261,317 - -0.7 261,317 - -0.1 261,317 - -2.4 261,317 - -0.7 261,469 + +1.7 261,469 + -2.3 261,470 - -0.0 261,527 + Psest_0250 0.12 -3.1 261,527 + Psest_0250 0.12 -1.1 261,527 + Psest_0250 0.12 -0.4 261,527 + Psest_0250 0.12 -2.9 261,527 + Psest_0250 0.12 +1.0 261,527 + Psest_0250 0.12 -1.0 261,528 - Psest_0250 0.12 -1.5 261,528 - Psest_0250 0.12 -0.7 261,528 - Psest_0250 0.12 -3.4 261,568 + Psest_0250 0.14 -1.5 261,568 + Psest_0250 0.14 -1.4 261,569 - Psest_0250 0.14 -1.6 261,578 - Psest_0250 0.14 +0.9 261,584 + Psest_0250 0.15 -0.9 261,584 + Psest_0250 0.15 -1.8 261,585 - Psest_0250 0.15 -0.8 261,594 + Psest_0250 0.15 -2.3 261,594 + Psest_0250 0.15 +0.3 261,594 + Psest_0250 0.15 +1.1 261,595 - Psest_0250 0.15 +0.1 261,595 - Psest_0250 0.15 -1.2 261,595 - Psest_0250 0.15 -0.7 261,829 + Psest_0250 0.26 -0.7 261,829 + Psest_0250 0.26 -1.0 261,829 + Psest_0250 0.26 -0.6 261,830 - Psest_0250 0.26 -1.0 261,830 - Psest_0250 0.26 +0.3 261,830 - Psest_0250 0.26 -0.2 261,830 - Psest_0250 0.26 +0.2 261,836 + Psest_0250 0.26 -1.5 261,869 - Psest_0250 0.28 -3.0 261,874 + Psest_0250 0.28 -0.7 261,874 + Psest_0250 0.28 -1.7 261,874 + Psest_0250 0.28 -1.9 261,874 + Psest_0250 0.28 -0.5 261,874 + Psest_0250 0.28 -2.8 261,874 + Psest_0250 0.28 +0.0 261,874 + Psest_0250 0.28 +1.1 261,874 + Psest_0250 0.28 -0.0 261,874 + Psest_0250 0.28 +0.3 261,874 + Psest_0250 0.28 +0.5 261,875 - Psest_0250 0.28 -1.9 261,875 - Psest_0250 0.28 -0.4 261,875 - Psest_0250 0.28 +1.1 261,875 - Psest_0250 0.28 -1.8 262,061 + Psest_0250 0.36 -0.8 262,061 + Psest_0250 0.36 -4.0 262,082 - Psest_0250 0.37 -1.1 262,082 - Psest_0250 0.37 -0.7 262,124 + Psest_0250 0.39 -1.8 262,124 + Psest_0250 0.39 -1.8 262,124 + Psest_0250 0.39 -1.6 262,124 + Psest_0250 0.39 -1.6 262,125 - Psest_0250 0.39 -0.5 262,125 - Psest_0250 0.39 -2.3 262,125 - Psest_0250 0.39 -0.4 262,125 - Psest_0250 0.39 -0.6 262,125 - Psest_0250 0.39 -1.5 262,208 + Psest_0250 0.43 -2.0 262,218 - Psest_0250 0.44 -0.8
Or see this region's nucleotide sequence