Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2369 and Psest_2370 overlap by 12 nucleotides Psest_2370 and Psest_2371 are separated by 336 nucleotides Psest_2371 and Psest_2372 are separated by 128 nucleotides Psest_2372 and Psest_2373 are separated by 90 nucleotides Psest_2373 and Psest_2374 overlap by 1 nucleotides Psest_2374 and Psest_2375 overlap by 1 nucleotides
Psest_2369: Psest_2369 - Transcriptional regulator, at 2,523,321 to 2,523,941
_2369
Psest_2370: Psest_2370 - Protein of unknown function (DUF1652)., at 2,523,930 to 2,524,202
_2370
Psest_2371: Psest_2371 - tRNA_Ser_TGA, at 2,524,539 to 2,524,628
_2371
Psest_2372: Psest_2372 - Integral membrane protein, interacts with FtsH, at 2,524,757 to 2,525,425
_2372
Psest_2373: Psest_2373 - sulfur relay protein TusD/DsrE, at 2,525,516 to 2,525,908
_2373
Psest_2374: Psest_2374 - sulfur relay protein TusC/DsrF, at 2,525,908 to 2,526,267
_2374
Psest_2375: Psest_2375 - sulfur relay protein TusB/DsrH, at 2,526,267 to 2,526,569
_2375
Position (kb)
2524
2525
2526 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2523.802 kb on - strand, within Psest_2369 at 2523.802 kb on - strand, within Psest_2369 at 2523.802 kb on - strand, within Psest_2369 at 2523.802 kb on - strand, within Psest_2369 at 2524.042 kb on - strand, within Psest_2370 at 2524.153 kb on + strand, within Psest_2370 at 2524.154 kb on - strand, within Psest_2370 at 2524.154 kb on - strand, within Psest_2370 at 2524.156 kb on + strand, within Psest_2370 at 2524.157 kb on - strand, within Psest_2370 at 2524.237 kb on - strand at 2524.237 kb on - strand at 2524.290 kb on + strand at 2524.472 kb on + strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.663 kb on - strand at 2524.684 kb on - strand at 2524.706 kb on - strand at 2524.833 kb on - strand, within Psest_2372 at 2524.833 kb on - strand, within Psest_2372 at 2524.937 kb on + strand, within Psest_2372 at 2524.938 kb on - strand, within Psest_2372 at 2525.044 kb on + strand, within Psest_2372 at 2525.044 kb on + strand, within Psest_2372 at 2525.044 kb on + strand, within Psest_2372 at 2525.045 kb on - strand, within Psest_2372 at 2525.045 kb on - strand, within Psest_2372 at 2525.045 kb on - strand, within Psest_2372 at 2525.045 kb on - strand, within Psest_2372 at 2525.045 kb on - strand, within Psest_2372 at 2525.045 kb on - strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.048 kb on + strand, within Psest_2372 at 2525.049 kb on - strand, within Psest_2372 at 2525.049 kb on - strand, within Psest_2372 at 2525.049 kb on - strand, within Psest_2372 at 2525.129 kb on + strand, within Psest_2372 at 2525.129 kb on + strand, within Psest_2372 at 2525.129 kb on + strand, within Psest_2372 at 2525.130 kb on - strand, within Psest_2372 at 2525.130 kb on - strand, within Psest_2372 at 2525.130 kb on - strand, within Psest_2372 at 2525.130 kb on - strand, within Psest_2372 at 2525.330 kb on - strand, within Psest_2372 at 2525.330 kb on - strand, within Psest_2372 at 2525.333 kb on + strand, within Psest_2372 at 2525.333 kb on + strand, within Psest_2372 at 2525.333 kb on + strand, within Psest_2372 at 2525.333 kb on + strand, within Psest_2372 at 2525.334 kb on - strand, within Psest_2372 at 2525.334 kb on - strand, within Psest_2372 at 2525.369 kb on + strand at 2525.370 kb on - strand at 2525.484 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 2,523,802 - Psest_2369 0.77 +0.2 2,523,802 - Psest_2369 0.77 -1.2 2,523,802 - Psest_2369 0.77 -1.6 2,523,802 - Psest_2369 0.77 +0.3 2,524,042 - Psest_2370 0.41 +1.3 2,524,153 + Psest_2370 0.82 -0.1 2,524,154 - Psest_2370 0.82 +1.3 2,524,154 - Psest_2370 0.82 -0.7 2,524,156 + Psest_2370 0.83 -1.1 2,524,157 - Psest_2370 0.83 +1.3 2,524,237 - -2.1 2,524,237 - -1.3 2,524,290 + -0.8 2,524,472 + +0.5 2,524,663 - -0.3 2,524,663 - -2.0 2,524,663 - -1.3 2,524,663 - -0.0 2,524,663 - -2.5 2,524,663 - -3.9 2,524,663 - -2.6 2,524,663 - -2.4 2,524,663 - -1.0 2,524,684 - -2.9 2,524,706 - -0.9 2,524,833 - Psest_2372 0.11 -1.5 2,524,833 - Psest_2372 0.11 -1.3 2,524,937 + Psest_2372 0.27 +0.3 2,524,938 - Psest_2372 0.27 -1.4 2,525,044 + Psest_2372 0.43 -2.7 2,525,044 + Psest_2372 0.43 -2.8 2,525,044 + Psest_2372 0.43 -0.5 2,525,045 - Psest_2372 0.43 -2.3 2,525,045 - Psest_2372 0.43 -1.5 2,525,045 - Psest_2372 0.43 -1.3 2,525,045 - Psest_2372 0.43 -1.3 2,525,045 - Psest_2372 0.43 -0.0 2,525,045 - Psest_2372 0.43 +2.0 2,525,048 + Psest_2372 0.43 -3.3 2,525,048 + Psest_2372 0.43 -2.9 2,525,048 + Psest_2372 0.43 -1.0 2,525,048 + Psest_2372 0.43 -1.3 2,525,048 + Psest_2372 0.43 -2.8 2,525,048 + Psest_2372 0.43 -1.7 2,525,048 + Psest_2372 0.43 +0.7 2,525,048 + Psest_2372 0.43 -0.6 2,525,048 + Psest_2372 0.43 -1.7 2,525,048 + Psest_2372 0.43 -0.3 2,525,048 + Psest_2372 0.43 -1.4 2,525,048 + Psest_2372 0.43 -2.8 2,525,048 + Psest_2372 0.43 -1.1 2,525,048 + Psest_2372 0.43 -1.0 2,525,048 + Psest_2372 0.43 -2.3 2,525,048 + Psest_2372 0.43 -1.7 2,525,049 - Psest_2372 0.44 -0.3 2,525,049 - Psest_2372 0.44 -1.6 2,525,049 - Psest_2372 0.44 -2.0 2,525,129 + Psest_2372 0.56 -1.4 2,525,129 + Psest_2372 0.56 -3.6 2,525,129 + Psest_2372 0.56 -2.3 2,525,130 - Psest_2372 0.56 -3.4 2,525,130 - Psest_2372 0.56 -0.3 2,525,130 - Psest_2372 0.56 -1.5 2,525,130 - Psest_2372 0.56 -2.5 2,525,330 - Psest_2372 0.86 -2.3 2,525,330 - Psest_2372 0.86 +0.7 2,525,333 + Psest_2372 0.86 -1.3 2,525,333 + Psest_2372 0.86 -2.9 2,525,333 + Psest_2372 0.86 -1.3 2,525,333 + Psest_2372 0.86 -0.3 2,525,334 - Psest_2372 0.86 -3.6 2,525,334 - Psest_2372 0.86 -1.8 2,525,369 + -1.3 2,525,370 - -2.4 2,525,484 + -0.7
Or see this region's nucleotide sequence