Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0021 and Psest_0022 overlap by 4 nucleotides Psest_0022 and Psest_0023 are separated by 55 nucleotides Psest_0023 and Psest_0024 are separated by 148 nucleotides
Psest_0021: Psest_0021 - ribosomal RNA small subunit methyltransferase RsmB, at 21,703 to 23,025
_0021
Psest_0022: Psest_0022 - methionyl-tRNA formyltransferase, at 23,022 to 23,966
_0022
Psest_0023: Psest_0023 - peptide deformylase, at 24,022 to 24,528
_0023
Psest_0024: Psest_0024 - LysM domain., at 24,677 to 25,702
_0024
Position (kb) 24
25 Strain fitness (log2 ratio) -2
-1
0
1
2 at 24.529 kb on - strand at 24.529 kb on - strand at 24.555 kb on - strand at 24.555 kb on - strand at 24.574 kb on - strand at 24.574 kb on - strand at 24.574 kb on - strand at 24.659 kb on - strand at 24.659 kb on - strand at 24.659 kb on - strand at 24.659 kb on - strand at 24.659 kb on - strand at 24.662 kb on - strand at 24.662 kb on - strand at 24.662 kb on - strand at 24.768 kb on - strand at 24.769 kb on + strand at 24.769 kb on + strand at 24.769 kb on + strand at 24.769 kb on + strand at 24.884 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.911 kb on + strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.912 kb on - strand, within Psest_0024 at 24.936 kb on + strand, within Psest_0024 at 24.937 kb on - strand, within Psest_0024 at 24.988 kb on + strand, within Psest_0024 at 24.988 kb on + strand, within Psest_0024 at 25.097 kb on + strand, within Psest_0024 at 25.097 kb on + strand, within Psest_0024 at 25.097 kb on + strand, within Psest_0024 at 25.097 kb on + strand, within Psest_0024 at 25.097 kb on + strand, within Psest_0024 at 25.098 kb on - strand, within Psest_0024 at 25.098 kb on - strand, within Psest_0024 at 25.151 kb on + strand, within Psest_0024 at 25.151 kb on + strand, within Psest_0024 at 25.152 kb on - strand, within Psest_0024 at 25.152 kb on - strand, within Psest_0024 at 25.214 kb on + strand, within Psest_0024 at 25.214 kb on + strand, within Psest_0024 at 25.214 kb on + strand, within Psest_0024 at 25.214 kb on + strand, within Psest_0024 at 25.215 kb on - strand, within Psest_0024 at 25.215 kb on - strand, within Psest_0024 at 25.215 kb on - strand, within Psest_0024 at 25.215 kb on - strand, within Psest_0024 at 25.215 kb on - strand, within Psest_0024 at 25.215 kb on - strand, within Psest_0024 at 25.215 kb on - strand, within Psest_0024
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 24,529 - -1.5 24,529 - +0.9 24,555 - -0.7 24,555 - -1.6 24,574 - +0.9 24,574 - -0.9 24,574 - -0.1 24,659 - -0.1 24,659 - +0.3 24,659 - +0.3 24,659 - +0.9 24,659 - +0.9 24,662 - -1.1 24,662 - +0.1 24,662 - -1.7 24,768 - -0.1 24,769 + -1.5 24,769 + -0.5 24,769 + -0.9 24,769 + +0.1 24,884 + Psest_0024 0.20 -0.1 24,911 + Psest_0024 0.23 +1.4 24,911 + Psest_0024 0.23 +1.9 24,911 + Psest_0024 0.23 +1.4 24,911 + Psest_0024 0.23 -1.9 24,911 + Psest_0024 0.23 -0.7 24,911 + Psest_0024 0.23 -0.1 24,911 + Psest_0024 0.23 -2.1 24,911 + Psest_0024 0.23 +0.6 24,911 + Psest_0024 0.23 -0.7 24,911 + Psest_0024 0.23 +0.6 24,911 + Psest_0024 0.23 -0.7 24,912 - Psest_0024 0.23 +1.4 24,912 - Psest_0024 0.23 +0.9 24,912 - Psest_0024 0.23 +1.5 24,912 - Psest_0024 0.23 +0.4 24,912 - Psest_0024 0.23 -0.7 24,912 - Psest_0024 0.23 -2.1 24,912 - Psest_0024 0.23 -0.5 24,912 - Psest_0024 0.23 +0.6 24,912 - Psest_0024 0.23 -1.1 24,912 - Psest_0024 0.23 -0.1 24,912 - Psest_0024 0.23 -0.1 24,912 - Psest_0024 0.23 -0.5 24,936 + Psest_0024 0.25 +1.2 24,937 - Psest_0024 0.25 +1.9 24,988 + Psest_0024 0.30 +1.4 24,988 + Psest_0024 0.30 +0.2 25,097 + Psest_0024 0.41 -0.6 25,097 + Psest_0024 0.41 +0.5 25,097 + Psest_0024 0.41 +0.9 25,097 + Psest_0024 0.41 +0.4 25,097 + Psest_0024 0.41 -0.5 25,098 - Psest_0024 0.41 -0.1 25,098 - Psest_0024 0.41 +0.9 25,151 + Psest_0024 0.46 +1.4 25,151 + Psest_0024 0.46 -1.9 25,152 - Psest_0024 0.46 +1.8 25,152 - Psest_0024 0.46 +0.1 25,214 + Psest_0024 0.52 -0.7 25,214 + Psest_0024 0.52 +1.9 25,214 + Psest_0024 0.52 +0.3 25,214 + Psest_0024 0.52 +0.5 25,215 - Psest_0024 0.52 -0.7 25,215 - Psest_0024 0.52 -0.1 25,215 - Psest_0024 0.52 -0.7 25,215 - Psest_0024 0.52 +1.7 25,215 - Psest_0024 0.52 -0.1 25,215 - Psest_0024 0.52 -0.9 25,215 - Psest_0024 0.52 +0.4
Or see this region's nucleotide sequence