Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2027 and Psest_2028 are separated by 124 nucleotides Psest_2028 and Psest_2029 are separated by 100 nucleotides Psest_2029 and Psest_2030 are separated by 52 nucleotides Psest_2030 and Psest_2031 are separated by 51 nucleotides
Psest_2027: Psest_2027 - ATP-dependent Clp protease ATP-binding subunit clpA, at 2,146,675 to 2,148,945
_2027
Psest_2028: Psest_2028 - translation initiation factor IF-1, at 2,149,070 to 2,149,288
_2028
Psest_2029: Psest_2029 - Putative arginyl-tRNA:protein arginylyltransferase, at 2,149,389 to 2,150,096
_2029
Psest_2030: Psest_2030 - leucyl/phenylalanyl-tRNA--protein transferase, at 2,150,149 to 2,150,829
_2030
Psest_2031: Psest_2031 - thioredoxin-disulfide reductase, at 2,150,881 to 2,151,828
_2031
Position (kb)
2149
2150
2151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2148.477 kb on + strand, within Psest_2027 at 2148.543 kb on + strand, within Psest_2027 at 2148.543 kb on + strand, within Psest_2027 at 2148.621 kb on + strand, within Psest_2027 at 2148.769 kb on + strand at 2148.769 kb on + strand at 2149.450 kb on + strand at 2149.451 kb on - strand at 2149.451 kb on - strand at 2149.451 kb on - strand at 2149.579 kb on + strand, within Psest_2029 at 2149.588 kb on + strand, within Psest_2029 at 2149.588 kb on + strand, within Psest_2029 at 2149.589 kb on - strand, within Psest_2029 at 2149.607 kb on + strand, within Psest_2029 at 2149.700 kb on + strand, within Psest_2029 at 2149.700 kb on + strand, within Psest_2029 at 2149.726 kb on + strand, within Psest_2029 at 2149.726 kb on + strand, within Psest_2029 at 2149.727 kb on - strand, within Psest_2029 at 2149.731 kb on - strand, within Psest_2029 at 2149.758 kb on - strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.768 kb on + strand, within Psest_2029 at 2149.768 kb on + strand, within Psest_2029 at 2149.768 kb on + strand, within Psest_2029 at 2149.769 kb on - strand, within Psest_2029 at 2149.769 kb on - strand, within Psest_2029 at 2149.775 kb on + strand, within Psest_2029 at 2149.775 kb on + strand, within Psest_2029 at 2149.776 kb on - strand, within Psest_2029 at 2149.777 kb on + strand, within Psest_2029 at 2149.778 kb on - strand, within Psest_2029 at 2149.975 kb on + strand, within Psest_2029 at 2150.041 kb on + strand at 2150.041 kb on + strand at 2150.042 kb on - strand at 2150.042 kb on - strand at 2150.042 kb on - strand at 2150.127 kb on + strand at 2150.127 kb on + strand at 2150.127 kb on + strand at 2150.127 kb on + strand at 2150.127 kb on + strand at 2150.127 kb on + strand at 2150.128 kb on - strand at 2150.128 kb on - strand at 2150.169 kb on + strand at 2150.170 kb on - strand at 2150.305 kb on + strand, within Psest_2030 at 2150.306 kb on - strand, within Psest_2030 at 2150.371 kb on + strand, within Psest_2030 at 2150.468 kb on + strand, within Psest_2030 at 2150.468 kb on + strand, within Psest_2030 at 2150.468 kb on + strand, within Psest_2030 at 2150.468 kb on + strand, within Psest_2030 at 2150.469 kb on - strand, within Psest_2030 at 2150.469 kb on - strand, within Psest_2030 at 2150.469 kb on - strand, within Psest_2030 at 2150.469 kb on - strand, within Psest_2030 at 2150.773 kb on + strand at 2150.773 kb on + strand at 2150.773 kb on + strand at 2150.773 kb on + strand at 2150.774 kb on - strand at 2150.886 kb on + strand at 2150.886 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 2,148,477 + Psest_2027 0.79 +0.3 2,148,543 + Psest_2027 0.82 +1.8 2,148,543 + Psest_2027 0.82 +2.5 2,148,621 + Psest_2027 0.86 +0.6 2,148,769 + +0.4 2,148,769 + +1.2 2,149,450 + -3.7 2,149,451 - -0.8 2,149,451 - -2.3 2,149,451 - -1.9 2,149,579 + Psest_2029 0.27 -0.1 2,149,588 + Psest_2029 0.28 -1.4 2,149,588 + Psest_2029 0.28 -1.1 2,149,589 - Psest_2029 0.28 -0.4 2,149,607 + Psest_2029 0.31 -2.6 2,149,700 + Psest_2029 0.44 -2.3 2,149,700 + Psest_2029 0.44 +1.0 2,149,726 + Psest_2029 0.48 -2.0 2,149,726 + Psest_2029 0.48 -1.2 2,149,727 - Psest_2029 0.48 -3.0 2,149,731 - Psest_2029 0.48 -0.4 2,149,758 - Psest_2029 0.52 -0.7 2,149,759 + Psest_2029 0.52 +0.7 2,149,759 + Psest_2029 0.52 -2.2 2,149,759 + Psest_2029 0.52 +0.3 2,149,759 + Psest_2029 0.52 -0.7 2,149,759 + Psest_2029 0.52 -1.4 2,149,759 + Psest_2029 0.52 -3.0 2,149,759 + Psest_2029 0.52 -1.8 2,149,759 + Psest_2029 0.52 -0.9 2,149,759 + Psest_2029 0.52 -2.8 2,149,760 - Psest_2029 0.52 -1.1 2,149,760 - Psest_2029 0.52 -1.1 2,149,760 - Psest_2029 0.52 -1.1 2,149,760 - Psest_2029 0.52 -1.4 2,149,760 - Psest_2029 0.52 -1.7 2,149,768 + Psest_2029 0.54 -0.6 2,149,768 + Psest_2029 0.54 +0.0 2,149,768 + Psest_2029 0.54 -2.4 2,149,769 - Psest_2029 0.54 -2.6 2,149,769 - Psest_2029 0.54 -2.6 2,149,775 + Psest_2029 0.55 -1.9 2,149,775 + Psest_2029 0.55 -0.2 2,149,776 - Psest_2029 0.55 -0.1 2,149,777 + Psest_2029 0.55 -0.6 2,149,778 - Psest_2029 0.55 -2.3 2,149,975 + Psest_2029 0.83 -0.6 2,150,041 + -2.4 2,150,041 + -0.9 2,150,042 - -3.5 2,150,042 - +0.7 2,150,042 - -1.7 2,150,127 + -1.9 2,150,127 + +0.4 2,150,127 + +0.7 2,150,127 + -0.5 2,150,127 + -1.5 2,150,127 + -0.6 2,150,128 - -0.1 2,150,128 - -1.6 2,150,169 + -1.0 2,150,170 - -2.2 2,150,305 + Psest_2030 0.23 -1.8 2,150,306 - Psest_2030 0.23 -0.9 2,150,371 + Psest_2030 0.33 -0.6 2,150,468 + Psest_2030 0.47 +0.2 2,150,468 + Psest_2030 0.47 -0.8 2,150,468 + Psest_2030 0.47 +0.8 2,150,468 + Psest_2030 0.47 -0.4 2,150,469 - Psest_2030 0.47 -0.1 2,150,469 - Psest_2030 0.47 -0.5 2,150,469 - Psest_2030 0.47 -1.5 2,150,469 - Psest_2030 0.47 +2.6 2,150,773 + -1.0 2,150,773 + -0.6 2,150,773 + +1.0 2,150,773 + +0.3 2,150,774 - +0.5 2,150,886 + -0.1 2,150,886 + -3.1
Or see this region's nucleotide sequence