Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2008 and Psest_2009 are separated by 135 nucleotides Psest_2009 and Psest_2010 are separated by 564 nucleotides Psest_2010 and Psest_2011 are separated by 218 nucleotides
Psest_2008: Psest_2008 - 23S rRNA, at 2,123,852 to 2,126,738
_2008
Psest_2009: Psest_2009 - 5S rRNA, at 2,126,874 to 2,126,989
_2009
Psest_2010: Psest_2010 - queuosine biosynthesis protein QueD, at 2,127,554 to 2,127,925
_2010
Psest_2011: Psest_2011 - Predicted permease, at 2,128,144 to 2,129,460
_2011
Position (kb)
2127
2128 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2127.180 kb on + strand at 2127.180 kb on + strand at 2127.180 kb on + strand at 2127.183 kb on + strand at 2127.183 kb on + strand at 2127.240 kb on - strand at 2127.258 kb on + strand at 2127.258 kb on + strand at 2127.258 kb on + strand at 2127.259 kb on - strand at 2127.275 kb on - strand at 2127.306 kb on + strand at 2127.307 kb on - strand at 2127.338 kb on - strand at 2127.342 kb on - strand at 2127.343 kb on + strand at 2127.343 kb on + strand at 2127.344 kb on - strand at 2127.378 kb on + strand at 2127.379 kb on - strand at 2127.379 kb on - strand at 2127.380 kb on + strand at 2127.380 kb on + strand at 2127.452 kb on + strand at 2127.452 kb on + strand at 2127.456 kb on + strand at 2127.456 kb on + strand at 2127.456 kb on + strand at 2127.457 kb on - strand at 2127.525 kb on + strand at 2127.525 kb on + strand at 2127.777 kb on - strand, within Psest_2010 at 2127.960 kb on + strand at 2128.008 kb on - strand at 2128.009 kb on + strand at 2128.009 kb on + strand at 2128.090 kb on + strand at 2128.190 kb on + strand at 2128.190 kb on + strand at 2128.205 kb on + strand at 2128.206 kb on - strand at 2128.206 kb on - strand at 2128.325 kb on - strand, within Psest_2011 at 2128.419 kb on + strand, within Psest_2011 at 2128.420 kb on - strand, within Psest_2011 at 2128.420 kb on - strand, within Psest_2011 at 2128.440 kb on + strand, within Psest_2011 at 2128.492 kb on - strand, within Psest_2011 at 2128.615 kb on + strand, within Psest_2011 at 2128.615 kb on + strand, within Psest_2011 at 2128.616 kb on - strand, within Psest_2011 at 2128.616 kb on - strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.617 kb on + strand, within Psest_2011 at 2128.618 kb on - strand, within Psest_2011 at 2128.618 kb on - strand, within Psest_2011 at 2128.714 kb on - strand, within Psest_2011 at 2128.757 kb on + strand, within Psest_2011 at 2128.758 kb on - strand, within Psest_2011 at 2128.771 kb on + strand, within Psest_2011 at 2128.771 kb on + strand, within Psest_2011 at 2128.771 kb on + strand, within Psest_2011 at 2128.830 kb on + strand, within Psest_2011 at 2128.831 kb on - strand, within Psest_2011
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 2,127,180 + -0.7 2,127,180 + -1.9 2,127,180 + +0.4 2,127,183 + +0.3 2,127,183 + +1.0 2,127,240 - -1.8 2,127,258 + -2.0 2,127,258 + -0.9 2,127,258 + -1.9 2,127,259 - +0.3 2,127,275 - -0.3 2,127,306 + -0.3 2,127,307 - -0.4 2,127,338 - +0.6 2,127,342 - -0.6 2,127,343 + -0.6 2,127,343 + -1.9 2,127,344 - -2.9 2,127,378 + -0.2 2,127,379 - -0.3 2,127,379 - -1.1 2,127,380 + -0.9 2,127,380 + -0.1 2,127,452 + -1.2 2,127,452 + -1.3 2,127,456 + -0.1 2,127,456 + +0.8 2,127,456 + -0.6 2,127,457 - +0.8 2,127,525 + -2.0 2,127,525 + -1.4 2,127,777 - Psest_2010 0.60 -2.6 2,127,960 + -4.0 2,128,008 - -2.1 2,128,009 + -0.0 2,128,009 + -2.1 2,128,090 + -2.1 2,128,190 + -0.7 2,128,190 + -2.8 2,128,205 + -3.6 2,128,206 - -3.8 2,128,206 - -2.1 2,128,325 - Psest_2011 0.14 -0.8 2,128,419 + Psest_2011 0.21 -3.1 2,128,420 - Psest_2011 0.21 -2.6 2,128,420 - Psest_2011 0.21 -2.8 2,128,440 + Psest_2011 0.22 -1.6 2,128,492 - Psest_2011 0.26 -2.1 2,128,615 + Psest_2011 0.36 -1.0 2,128,615 + Psest_2011 0.36 -3.3 2,128,616 - Psest_2011 0.36 -2.6 2,128,616 - Psest_2011 0.36 -0.5 2,128,617 + Psest_2011 0.36 -3.0 2,128,617 + Psest_2011 0.36 -3.2 2,128,617 + Psest_2011 0.36 -3.1 2,128,617 + Psest_2011 0.36 -2.7 2,128,617 + Psest_2011 0.36 -2.3 2,128,617 + Psest_2011 0.36 -0.1 2,128,617 + Psest_2011 0.36 +1.0 2,128,617 + Psest_2011 0.36 -1.7 2,128,617 + Psest_2011 0.36 -4.9 2,128,617 + Psest_2011 0.36 -2.7 2,128,617 + Psest_2011 0.36 -0.7 2,128,617 + Psest_2011 0.36 -2.5 2,128,617 + Psest_2011 0.36 -3.9 2,128,617 + Psest_2011 0.36 -2.0 2,128,617 + Psest_2011 0.36 -2.9 2,128,617 + Psest_2011 0.36 -1.9 2,128,617 + Psest_2011 0.36 -2.7 2,128,618 - Psest_2011 0.36 -1.9 2,128,618 - Psest_2011 0.36 +0.2 2,128,714 - Psest_2011 0.43 -1.2 2,128,757 + Psest_2011 0.47 -0.7 2,128,758 - Psest_2011 0.47 -2.9 2,128,771 + Psest_2011 0.48 -2.9 2,128,771 + Psest_2011 0.48 -1.7 2,128,771 + Psest_2011 0.48 -3.4 2,128,830 + Psest_2011 0.52 -1.9 2,128,831 - Psest_2011 0.52 -0.4
Or see this region's nucleotide sequence