Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1851 and Psest_1852 are separated by 48 nucleotides Psest_1852 and Psest_1853 are separated by 50 nucleotides Psest_1853 and Psest_1854 are separated by 145 nucleotides
Psest_1851: Psest_1851 - Uncharacterized protein conserved in bacteria, at 1,955,574 to 1,956,752
_1851
Psest_1852: Psest_1852 - Uncharacterized conserved protein, at 1,956,801 to 1,957,205
_1852
Psest_1853: Psest_1853 - MoxR-like ATPases, at 1,957,256 to 1,958,104
_1853
Psest_1854: Psest_1854 - Domain of Unknown Function (DUF748)., at 1,958,250 to 1,959,320
_1854
Position (kb)
1957
1958
1959 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1956.525 kb on + strand, within Psest_1851 at 1956.525 kb on + strand, within Psest_1851 at 1956.525 kb on + strand, within Psest_1851 at 1956.526 kb on - strand, within Psest_1851 at 1956.526 kb on - strand, within Psest_1851 at 1956.526 kb on - strand, within Psest_1851 at 1956.559 kb on + strand, within Psest_1851 at 1956.631 kb on + strand, within Psest_1851 at 1956.631 kb on + strand, within Psest_1851 at 1956.631 kb on + strand, within Psest_1851 at 1956.632 kb on - strand, within Psest_1851 at 1956.632 kb on - strand, within Psest_1851 at 1956.874 kb on + strand, within Psest_1852 at 1956.874 kb on + strand, within Psest_1852 at 1956.875 kb on - strand, within Psest_1852 at 1956.875 kb on - strand, within Psest_1852 at 1956.875 kb on - strand, within Psest_1852 at 1956.875 kb on - strand, within Psest_1852 at 1956.875 kb on - strand, within Psest_1852 at 1956.878 kb on + strand, within Psest_1852 at 1956.878 kb on + strand, within Psest_1852 at 1956.879 kb on - strand, within Psest_1852 at 1956.879 kb on - strand, within Psest_1852 at 1956.879 kb on - strand, within Psest_1852 at 1956.879 kb on - strand, within Psest_1852 at 1957.019 kb on - strand, within Psest_1852 at 1957.578 kb on + strand, within Psest_1853 at 1957.578 kb on + strand, within Psest_1853 at 1957.578 kb on + strand, within Psest_1853 at 1957.578 kb on + strand, within Psest_1853 at 1957.579 kb on - strand, within Psest_1853 at 1957.579 kb on - strand, within Psest_1853 at 1957.686 kb on + strand, within Psest_1853 at 1957.692 kb on + strand, within Psest_1853 at 1957.693 kb on - strand, within Psest_1853 at 1957.693 kb on - strand, within Psest_1853 at 1957.693 kb on - strand, within Psest_1853 at 1957.693 kb on - strand, within Psest_1853 at 1957.824 kb on + strand, within Psest_1853 at 1957.824 kb on + strand, within Psest_1853 at 1957.824 kb on + strand, within Psest_1853 at 1957.824 kb on + strand, within Psest_1853 at 1957.824 kb on + strand, within Psest_1853 at 1957.824 kb on + strand, within Psest_1853 at 1957.824 kb on + strand, within Psest_1853 at 1957.825 kb on - strand, within Psest_1853 at 1957.825 kb on - strand, within Psest_1853 at 1957.825 kb on - strand, within Psest_1853 at 1957.825 kb on - strand, within Psest_1853 at 1957.890 kb on + strand, within Psest_1853 at 1957.890 kb on + strand, within Psest_1853 at 1957.891 kb on - strand, within Psest_1853 at 1957.891 kb on - strand, within Psest_1853 at 1958.079 kb on + strand at 1958.079 kb on + strand at 1958.080 kb on - strand at 1958.080 kb on - strand at 1958.080 kb on - strand at 1958.080 kb on - strand at 1958.080 kb on - strand at 1958.080 kb on - strand at 1958.147 kb on + strand at 1958.148 kb on - strand at 1958.163 kb on + strand at 1958.163 kb on + strand at 1958.163 kb on + strand at 1958.165 kb on + strand at 1958.166 kb on - strand at 1958.166 kb on - strand at 1958.252 kb on - strand at 1958.411 kb on + strand, within Psest_1854 at 1958.412 kb on - strand, within Psest_1854 at 1958.494 kb on + strand, within Psest_1854 at 1958.533 kb on + strand, within Psest_1854 at 1958.534 kb on - strand, within Psest_1854 at 1958.534 kb on - strand, within Psest_1854 at 1958.534 kb on - strand, within Psest_1854 at 1958.534 kb on - strand, within Psest_1854 at 1958.647 kb on - strand, within Psest_1854 at 1958.803 kb on + strand, within Psest_1854 at 1958.804 kb on - strand, within Psest_1854 at 1958.804 kb on - strand, within Psest_1854
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 1,956,525 + Psest_1851 0.81 -0.9 1,956,525 + Psest_1851 0.81 +0.5 1,956,525 + Psest_1851 0.81 +0.4 1,956,526 - Psest_1851 0.81 -0.3 1,956,526 - Psest_1851 0.81 -0.6 1,956,526 - Psest_1851 0.81 -0.5 1,956,559 + Psest_1851 0.84 -0.9 1,956,631 + Psest_1851 0.90 -0.4 1,956,631 + Psest_1851 0.90 -0.6 1,956,631 + Psest_1851 0.90 -0.7 1,956,632 - Psest_1851 0.90 +0.2 1,956,632 - Psest_1851 0.90 -0.2 1,956,874 + Psest_1852 0.18 +0.4 1,956,874 + Psest_1852 0.18 +0.7 1,956,875 - Psest_1852 0.18 +0.1 1,956,875 - Psest_1852 0.18 -0.6 1,956,875 - Psest_1852 0.18 +0.7 1,956,875 - Psest_1852 0.18 -1.5 1,956,875 - Psest_1852 0.18 -0.5 1,956,878 + Psest_1852 0.19 -2.2 1,956,878 + Psest_1852 0.19 +0.6 1,956,879 - Psest_1852 0.19 +0.1 1,956,879 - Psest_1852 0.19 -2.0 1,956,879 - Psest_1852 0.19 +0.8 1,956,879 - Psest_1852 0.19 -0.6 1,957,019 - Psest_1852 0.54 +0.4 1,957,578 + Psest_1853 0.38 -1.0 1,957,578 + Psest_1853 0.38 -1.1 1,957,578 + Psest_1853 0.38 -2.4 1,957,578 + Psest_1853 0.38 -0.5 1,957,579 - Psest_1853 0.38 +0.1 1,957,579 - Psest_1853 0.38 +0.5 1,957,686 + Psest_1853 0.51 +0.0 1,957,692 + Psest_1853 0.51 +0.3 1,957,693 - Psest_1853 0.51 +0.1 1,957,693 - Psest_1853 0.51 -2.8 1,957,693 - Psest_1853 0.51 +0.7 1,957,693 - Psest_1853 0.51 -0.9 1,957,824 + Psest_1853 0.67 -1.5 1,957,824 + Psest_1853 0.67 +0.0 1,957,824 + Psest_1853 0.67 +0.5 1,957,824 + Psest_1853 0.67 -0.9 1,957,824 + Psest_1853 0.67 -1.7 1,957,824 + Psest_1853 0.67 -0.9 1,957,824 + Psest_1853 0.67 -2.2 1,957,825 - Psest_1853 0.67 -0.7 1,957,825 - Psest_1853 0.67 -0.7 1,957,825 - Psest_1853 0.67 -2.2 1,957,825 - Psest_1853 0.67 -1.8 1,957,890 + Psest_1853 0.75 +0.2 1,957,890 + Psest_1853 0.75 -1.4 1,957,891 - Psest_1853 0.75 -1.5 1,957,891 - Psest_1853 0.75 -2.3 1,958,079 + -2.5 1,958,079 + -0.9 1,958,080 - -0.6 1,958,080 - -0.6 1,958,080 - -2.0 1,958,080 - -0.2 1,958,080 - -0.9 1,958,080 - -3.4 1,958,147 + -0.8 1,958,148 - -0.0 1,958,163 + -0.6 1,958,163 + +0.4 1,958,163 + -0.6 1,958,165 + -0.6 1,958,166 - -1.0 1,958,166 - -0.3 1,958,252 - -1.0 1,958,411 + Psest_1854 0.15 -0.6 1,958,412 - Psest_1854 0.15 -0.8 1,958,494 + Psest_1854 0.23 +0.4 1,958,533 + Psest_1854 0.26 +0.2 1,958,534 - Psest_1854 0.27 -0.8 1,958,534 - Psest_1854 0.27 -2.9 1,958,534 - Psest_1854 0.27 -1.2 1,958,534 - Psest_1854 0.27 -1.0 1,958,647 - Psest_1854 0.37 -0.3 1,958,803 + Psest_1854 0.52 -0.3 1,958,804 - Psest_1854 0.52 -1.4 1,958,804 - Psest_1854 0.52 +0.4
Or see this region's nucleotide sequence