Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1706 and Psest_1707 are separated by 125 nucleotides Psest_1707 and Psest_1708 are separated by 8 nucleotides Psest_1708 and Psest_1709 overlap by 4 nucleotides
Psest_1706: Psest_1706 - Electron transfer flavoprotein, alpha subunit, at 1,812,331 to 1,813,260
_1706
Psest_1707: Psest_1707 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,813,386 to 1,814,207
_1707
Psest_1708: Psest_1708 - hypothetical protein, at 1,814,216 to 1,814,572
_1708
Psest_1709: Psest_1709 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 1,814,569 to 1,815,366
_1709
Position (kb)
1813
1814
1815 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1812.432 kb on - strand, within Psest_1706 at 1812.732 kb on + strand, within Psest_1706 at 1813.294 kb on + strand at 1813.294 kb on + strand at 1813.294 kb on + strand at 1813.334 kb on + strand at 1813.334 kb on + strand at 1813.335 kb on - strand at 1813.335 kb on - strand at 1813.335 kb on - strand at 1813.608 kb on + strand, within Psest_1707 at 1813.608 kb on + strand, within Psest_1707 at 1813.608 kb on + strand, within Psest_1707 at 1813.609 kb on - strand, within Psest_1707 at 1813.609 kb on - strand, within Psest_1707 at 1813.642 kb on + strand, within Psest_1707 at 1813.664 kb on - strand, within Psest_1707 at 1813.735 kb on + strand, within Psest_1707 at 1813.735 kb on + strand, within Psest_1707 at 1813.736 kb on - strand, within Psest_1707 at 1813.776 kb on + strand, within Psest_1707 at 1813.776 kb on + strand, within Psest_1707 at 1813.935 kb on + strand, within Psest_1707 at 1813.936 kb on - strand, within Psest_1707 at 1813.936 kb on - strand, within Psest_1707 at 1814.150 kb on + strand at 1814.205 kb on + strand at 1814.206 kb on - strand at 1814.206 kb on - strand at 1814.241 kb on + strand at 1814.341 kb on - strand, within Psest_1708 at 1814.341 kb on - strand, within Psest_1708 at 1814.544 kb on + strand at 1814.545 kb on - strand at 1814.583 kb on - strand at 1814.612 kb on + strand at 1814.612 kb on + strand at 1814.613 kb on - strand at 1814.717 kb on + strand, within Psest_1709 at 1814.718 kb on - strand, within Psest_1709 at 1814.765 kb on - strand, within Psest_1709 at 1814.800 kb on + strand, within Psest_1709 at 1815.119 kb on + strand, within Psest_1709
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 1,812,432 - Psest_1706 0.11 -1.7 1,812,732 + Psest_1706 0.43 -1.0 1,813,294 + -0.1 1,813,294 + -2.3 1,813,294 + -0.6 1,813,334 + -2.2 1,813,334 + -1.8 1,813,335 - -0.1 1,813,335 - -0.1 1,813,335 - -0.3 1,813,608 + Psest_1707 0.27 -0.7 1,813,608 + Psest_1707 0.27 -0.4 1,813,608 + Psest_1707 0.27 -0.5 1,813,609 - Psest_1707 0.27 -0.4 1,813,609 - Psest_1707 0.27 +0.4 1,813,642 + Psest_1707 0.31 -0.8 1,813,664 - Psest_1707 0.34 -1.4 1,813,735 + Psest_1707 0.42 +0.2 1,813,735 + Psest_1707 0.42 -1.0 1,813,736 - Psest_1707 0.43 +3.6 1,813,776 + Psest_1707 0.47 +1.1 1,813,776 + Psest_1707 0.47 -0.8 1,813,935 + Psest_1707 0.67 -0.2 1,813,936 - Psest_1707 0.67 -3.0 1,813,936 - Psest_1707 0.67 +1.3 1,814,150 + +0.4 1,814,205 + +0.6 1,814,206 - +0.5 1,814,206 - +0.0 1,814,241 + -0.7 1,814,341 - Psest_1708 0.35 -0.9 1,814,341 - Psest_1708 0.35 -0.6 1,814,544 + +0.4 1,814,545 - +1.1 1,814,583 - -1.6 1,814,612 + +0.3 1,814,612 + +0.0 1,814,613 - -0.5 1,814,717 + Psest_1709 0.19 +0.2 1,814,718 - Psest_1709 0.19 -0.5 1,814,765 - Psest_1709 0.25 +0.4 1,814,800 + Psest_1709 0.29 +1.0 1,815,119 + Psest_1709 0.69 -0.7
Or see this region's nucleotide sequence