Experiment: Gentamicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1307 and Psest_1308 are separated by 164 nucleotides Psest_1308 and Psest_1309 are separated by 2 nucleotides Psest_1309 and Psest_1310 overlap by 4 nucleotides
Psest_1307: Psest_1307 - Dienelactone hydrolase and related enzymes, at 1,408,189 to 1,408,995
_1307
Psest_1308: Psest_1308 - ABC-type branched-chain amino acid transport systems, ATPase component, at 1,409,160 to 1,409,861
_1308
Psest_1309: Psest_1309 - ABC-type branched-chain amino acid transport systems, ATPase component, at 1,409,864 to 1,410,631
_1309
Psest_1310: Psest_1310 - ABC-type branched-chain amino acid transport system, permease component, at 1,410,628 to 1,411,884
_1310
Position (kb)
1409
1410
1411 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1408.880 kb on + strand, within Psest_1307 at 1408.880 kb on + strand, within Psest_1307 at 1408.881 kb on - strand, within Psest_1307 at 1408.881 kb on - strand, within Psest_1307 at 1408.884 kb on - strand, within Psest_1307 at 1408.917 kb on - strand at 1409.045 kb on + strand at 1409.173 kb on + strand at 1409.174 kb on - strand at 1409.174 kb on - strand at 1409.247 kb on + strand, within Psest_1308 at 1409.247 kb on + strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.252 kb on - strand, within Psest_1308 at 1409.252 kb on - strand, within Psest_1308 at 1409.377 kb on - strand, within Psest_1308 at 1409.830 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.866 kb on - strand at 1409.866 kb on - strand at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1410.166 kb on - strand, within Psest_1309 at 1410.302 kb on - strand, within Psest_1309 at 1410.383 kb on + strand, within Psest_1309 at 1410.384 kb on - strand, within Psest_1309 at 1410.469 kb on - strand, within Psest_1309 at 1410.548 kb on + strand, within Psest_1309 at 1410.549 kb on - strand, within Psest_1309 at 1410.608 kb on + strand at 1411.118 kb on + strand, within Psest_1310 at 1411.118 kb on + strand, within Psest_1310 at 1411.119 kb on - strand, within Psest_1310 at 1411.403 kb on + strand, within Psest_1310 at 1411.403 kb on + strand, within Psest_1310 at 1411.421 kb on + strand, within Psest_1310 at 1411.421 kb on + strand, within Psest_1310 at 1411.421 kb on + strand, within Psest_1310 at 1411.421 kb on + strand, within Psest_1310 at 1411.422 kb on - strand, within Psest_1310 at 1411.422 kb on - strand, within Psest_1310 at 1411.422 kb on - strand, within Psest_1310 at 1411.422 kb on - strand, within Psest_1310 at 1411.422 kb on - strand, within Psest_1310 at 1411.422 kb on - strand, within Psest_1310 at 1411.422 kb on - strand, within Psest_1310 at 1411.442 kb on + strand, within Psest_1310 at 1411.443 kb on - strand, within Psest_1310 at 1411.454 kb on + strand, within Psest_1310 at 1411.517 kb on + strand, within Psest_1310 at 1411.518 kb on - strand, within Psest_1310 at 1411.518 kb on - strand, within Psest_1310 at 1411.518 kb on - strand, within Psest_1310 at 1411.562 kb on + strand, within Psest_1310
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.0016 mg/ml remove 1,408,880 + Psest_1307 0.86 -0.1 1,408,880 + Psest_1307 0.86 +0.7 1,408,881 - Psest_1307 0.86 -1.9 1,408,881 - Psest_1307 0.86 -0.0 1,408,884 - Psest_1307 0.86 -1.1 1,408,917 - -0.7 1,409,045 + +1.4 1,409,173 + -1.0 1,409,174 - -0.2 1,409,174 - +0.0 1,409,247 + Psest_1308 0.12 -0.4 1,409,247 + Psest_1308 0.12 -0.7 1,409,248 - Psest_1308 0.13 +1.2 1,409,248 - Psest_1308 0.13 +1.3 1,409,248 - Psest_1308 0.13 +1.3 1,409,248 - Psest_1308 0.13 +0.4 1,409,248 - Psest_1308 0.13 +1.5 1,409,248 - Psest_1308 0.13 +2.1 1,409,248 - Psest_1308 0.13 +0.7 1,409,248 - Psest_1308 0.13 +0.7 1,409,252 - Psest_1308 0.13 +0.5 1,409,252 - Psest_1308 0.13 +1.3 1,409,377 - Psest_1308 0.31 +0.3 1,409,830 + -0.6 1,409,865 + -1.4 1,409,865 + +0.2 1,409,865 + -0.4 1,409,865 + +0.3 1,409,865 + -0.5 1,409,866 - +1.2 1,409,866 - +3.3 1,409,958 + Psest_1309 0.12 +0.9 1,409,958 + Psest_1309 0.12 +0.0 1,409,958 + Psest_1309 0.12 -0.6 1,409,958 + Psest_1309 0.12 -0.4 1,409,958 + Psest_1309 0.12 +0.6 1,409,958 + Psest_1309 0.12 -0.5 1,409,958 + Psest_1309 0.12 -0.2 1,409,958 + Psest_1309 0.12 +1.0 1,409,959 - Psest_1309 0.12 +0.1 1,409,959 - Psest_1309 0.12 -0.2 1,409,959 - Psest_1309 0.12 +1.2 1,409,959 - Psest_1309 0.12 +1.2 1,409,959 - Psest_1309 0.12 +1.4 1,409,959 - Psest_1309 0.12 +0.8 1,409,959 - Psest_1309 0.12 -0.4 1,409,959 - Psest_1309 0.12 +0.5 1,409,959 - Psest_1309 0.12 +1.2 1,409,959 - Psest_1309 0.12 +0.5 1,409,959 - Psest_1309 0.12 +0.7 1,409,959 - Psest_1309 0.12 +0.7 1,409,959 - Psest_1309 0.12 -0.4 1,410,166 - Psest_1309 0.39 +2.4 1,410,302 - Psest_1309 0.57 -0.3 1,410,383 + Psest_1309 0.68 -0.7 1,410,384 - Psest_1309 0.68 -1.2 1,410,469 - Psest_1309 0.79 +0.5 1,410,548 + Psest_1309 0.89 +0.9 1,410,549 - Psest_1309 0.89 -1.2 1,410,608 + -0.5 1,411,118 + Psest_1310 0.39 -0.4 1,411,118 + Psest_1310 0.39 -0.1 1,411,119 - Psest_1310 0.39 +1.3 1,411,403 + Psest_1310 0.62 +0.8 1,411,403 + Psest_1310 0.62 -2.7 1,411,421 + Psest_1310 0.63 -0.8 1,411,421 + Psest_1310 0.63 -0.3 1,411,421 + Psest_1310 0.63 +0.2 1,411,421 + Psest_1310 0.63 +1.5 1,411,422 - Psest_1310 0.63 -0.2 1,411,422 - Psest_1310 0.63 -1.6 1,411,422 - Psest_1310 0.63 -0.3 1,411,422 - Psest_1310 0.63 +0.7 1,411,422 - Psest_1310 0.63 +0.6 1,411,422 - Psest_1310 0.63 -0.2 1,411,422 - Psest_1310 0.63 +0.2 1,411,442 + Psest_1310 0.65 +0.7 1,411,443 - Psest_1310 0.65 +0.6 1,411,454 + Psest_1310 0.66 +0.5 1,411,517 + Psest_1310 0.71 +1.2 1,411,518 - Psest_1310 0.71 -1.5 1,411,518 - Psest_1310 0.71 -1.8 1,411,518 - Psest_1310 0.71 -0.3 1,411,562 + Psest_1310 0.74 -0.8
Or see this region's nucleotide sequence