Experiment: Chloramphenicol 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt greA and SO1192 are separated by 277 nucleotides SO1192 and secD-1 are separated by 295 nucleotides
SO1191: greA - transcription elongation factor GreA (NCBI ptt file), at 1,234,415 to 1,234,891
greA
SO1192: SO1192 - hypothetical protein (NCBI ptt file), at 1,235,169 to 1,235,696
SO1192
SO1193: secD-1 - protein-export membrane protein SecD (NCBI ptt file), at 1,235,992 to 1,237,848
secD-1
Position (kb)
1235
1236 Strain fitness (log2 ratio)
-2
-1
0
1 at 1234.332 kb on - strand at 1234.332 kb on - strand at 1234.366 kb on - strand at 1234.434 kb on - strand at 1234.451 kb on - strand at 1234.463 kb on + strand, within greA at 1234.474 kb on + strand, within greA at 1234.577 kb on - strand, within greA at 1234.928 kb on - strand at 1234.982 kb on + strand at 1235.040 kb on - strand at 1235.244 kb on + strand, within SO1192 at 1235.246 kb on + strand, within SO1192 at 1235.338 kb on + strand, within SO1192 at 1235.392 kb on + strand, within SO1192 at 1235.438 kb on - strand, within SO1192 at 1235.473 kb on + strand, within SO1192 at 1235.500 kb on + strand, within SO1192 at 1235.517 kb on + strand, within SO1192 at 1235.539 kb on - strand, within SO1192 at 1235.691 kb on + strand at 1235.691 kb on + strand at 1235.699 kb on - strand at 1235.699 kb on - strand at 1235.871 kb on + strand at 1235.898 kb on - strand at 1235.948 kb on - strand at 1236.070 kb on - strand at 1236.073 kb on + strand at 1236.126 kb on - strand at 1236.163 kb on - strand at 1236.181 kb on + strand, within secD-1 at 1236.188 kb on + strand, within secD-1 at 1236.280 kb on + strand, within secD-1 at 1236.287 kb on + strand, within secD-1 at 1236.311 kb on + strand, within secD-1 at 1236.336 kb on + strand, within secD-1 at 1236.453 kb on + strand, within secD-1 at 1236.459 kb on + strand, within secD-1 at 1236.462 kb on - strand, within secD-1 at 1236.468 kb on - strand, within secD-1 at 1236.537 kb on - strand, within secD-1 at 1236.576 kb on + strand, within secD-1 at 1236.576 kb on + strand, within secD-1 at 1236.584 kb on - strand, within secD-1 at 1236.636 kb on + strand, within secD-1
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0004 mg/ml remove 1,234,332 - -1.4 1,234,332 - +0.3 1,234,366 - +0.8 1,234,434 - -0.4 1,234,451 - -0.4 1,234,463 + greA SO1191 0.10 -2.0 1,234,474 + greA SO1191 0.12 -0.4 1,234,577 - greA SO1191 0.34 -0.7 1,234,928 - +0.5 1,234,982 + -2.0 1,235,040 - +0.2 1,235,244 + SO1192 0.14 +0.2 1,235,246 + SO1192 0.15 -0.2 1,235,338 + SO1192 0.32 +0.6 1,235,392 + SO1192 0.42 +0.1 1,235,438 - SO1192 0.51 +0.2 1,235,473 + SO1192 0.58 +1.0 1,235,500 + SO1192 0.63 -0.1 1,235,517 + SO1192 0.66 -0.7 1,235,539 - SO1192 0.70 -0.0 1,235,691 + +0.5 1,235,691 + +1.0 1,235,699 - +0.2 1,235,699 - -0.0 1,235,871 + -0.0 1,235,898 - +0.2 1,235,948 - -0.4 1,236,070 - -0.1 1,236,073 + -0.3 1,236,126 - +0.0 1,236,163 - -0.0 1,236,181 + secD-1 SO1193 0.10 -0.5 1,236,188 + secD-1 SO1193 0.11 -0.5 1,236,280 + secD-1 SO1193 0.16 +0.6 1,236,287 + secD-1 SO1193 0.16 +0.7 1,236,311 + secD-1 SO1193 0.17 -0.0 1,236,336 + secD-1 SO1193 0.19 -0.3 1,236,453 + secD-1 SO1193 0.25 +0.5 1,236,459 + secD-1 SO1193 0.25 +0.1 1,236,462 - secD-1 SO1193 0.25 -0.1 1,236,468 - secD-1 SO1193 0.26 -0.3 1,236,537 - secD-1 SO1193 0.29 -0.2 1,236,576 + secD-1 SO1193 0.31 +0.7 1,236,576 + secD-1 SO1193 0.31 +1.0 1,236,584 - secD-1 SO1193 0.32 -0.1 1,236,636 + secD-1 SO1193 0.35 +0.6
Or see this region's nucleotide sequence