Experiment: Chloramphenicol 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0034 and smg are separated by 2 nucleotides smg and SO0036 are separated by 112 nucleotides SO0036 and SO0037 are separated by 138 nucleotides SO0037 and hemF are separated by 28 nucleotides
SO0034: SO0034 - DNA processing protein DprA, putative (NCBI ptt file), at 39,244 to 40,260
SO0034
SO0035: smg - smg protein (NCBI ptt file), at 40,263 to 40,739
smg
SO0036: SO0036 - topoisomerase DNA-binding C4 zinc finger domain protein (NCBI ptt file), at 40,852 to 41,418
SO0036
SO0037: SO0037 - Sua5/YciO/YrdC/YwlC family protein (NCBI ptt file), at 41,557 to 42,123
SO0037
SO0038: hemF - coproporphyrinogen III oxidase, aerobic (NCBI ptt file), at 42,152 to 43,060
hemF
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1 at 39.880 kb on - strand, within SO0034 at 40.061 kb on + strand, within SO0034 at 40.227 kb on + strand at 40.234 kb on + strand at 40.234 kb on + strand at 40.239 kb on + strand at 40.239 kb on + strand at 40.246 kb on + strand at 40.247 kb on - strand at 40.252 kb on - strand at 40.335 kb on + strand, within smg at 40.344 kb on - strand, within smg at 40.350 kb on - strand, within smg at 40.362 kb on + strand, within smg at 40.430 kb on + strand, within smg at 40.430 kb on + strand, within smg at 40.435 kb on + strand, within smg at 40.435 kb on + strand, within smg at 40.435 kb on + strand, within smg at 40.435 kb on + strand, within smg at 40.435 kb on + strand, within smg at 40.436 kb on + strand, within smg at 40.436 kb on + strand, within smg at 40.436 kb on + strand, within smg at 40.438 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.443 kb on - strand, within smg at 40.445 kb on + strand, within smg at 40.511 kb on - strand, within smg at 40.564 kb on + strand, within smg at 40.660 kb on - strand, within smg at 40.727 kb on + strand at 40.735 kb on - strand at 40.750 kb on - strand at 40.878 kb on + strand at 40.978 kb on - strand, within SO0036 at 41.015 kb on - strand, within SO0036 at 41.023 kb on - strand, within SO0036 at 41.025 kb on + strand, within SO0036 at 41.057 kb on + strand, within SO0036 at 41.149 kb on - strand, within SO0036 at 41.180 kb on + strand, within SO0036 at 41.188 kb on - strand, within SO0036 at 41.216 kb on + strand, within SO0036 at 41.216 kb on + strand, within SO0036 at 41.360 kb on + strand, within SO0036 at 41.371 kb on + strand at 41.379 kb on - strand at 41.426 kb on - strand at 41.454 kb on + strand at 41.454 kb on - strand at 41.462 kb on - strand at 41.462 kb on - strand at 41.481 kb on + strand at 42.218 kb on + strand at 42.219 kb on + strand at 42.294 kb on + strand, within hemF at 42.308 kb on - strand, within hemF at 42.308 kb on - strand, within hemF at 42.398 kb on - strand, within hemF at 42.411 kb on + strand, within hemF
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0004 mg/ml remove 39,880 - SO0034 0.63 -0.2 40,061 + SO0034 0.80 +0.5 40,227 + +0.2 40,234 + -0.2 40,234 + +0.2 40,239 + +0.4 40,239 + -0.5 40,246 + +0.3 40,247 - +0.5 40,252 - +0.2 40,335 + smg SO0035 0.15 +0.1 40,344 - smg SO0035 0.17 -0.3 40,350 - smg SO0035 0.18 +0.0 40,362 + smg SO0035 0.21 -0.0 40,430 + smg SO0035 0.35 +0.4 40,430 + smg SO0035 0.35 -0.4 40,435 + smg SO0035 0.36 +0.0 40,435 + smg SO0035 0.36 -0.1 40,435 + smg SO0035 0.36 +0.0 40,435 + smg SO0035 0.36 -0.3 40,435 + smg SO0035 0.36 -0.5 40,436 + smg SO0035 0.36 -0.5 40,436 + smg SO0035 0.36 -0.3 40,436 + smg SO0035 0.36 -0.2 40,438 - smg SO0035 0.37 -0.0 40,443 - smg SO0035 0.38 -0.5 40,443 - smg SO0035 0.38 -1.1 40,443 - smg SO0035 0.38 -0.4 40,443 - smg SO0035 0.38 -0.6 40,443 - smg SO0035 0.38 +0.4 40,443 - smg SO0035 0.38 -0.3 40,443 - smg SO0035 0.38 +1.4 40,443 - smg SO0035 0.38 -0.3 40,443 - smg SO0035 0.38 -0.2 40,445 + smg SO0035 0.38 -0.9 40,511 - smg SO0035 0.52 +0.0 40,564 + smg SO0035 0.63 -0.6 40,660 - smg SO0035 0.83 -0.1 40,727 + -0.3 40,735 - +0.0 40,750 - +0.1 40,878 + +0.5 40,978 - SO0036 0.22 -1.0 41,015 - SO0036 0.29 -0.2 41,023 - SO0036 0.30 +0.2 41,025 + SO0036 0.31 -1.0 41,057 + SO0036 0.36 -0.2 41,149 - SO0036 0.52 -0.2 41,180 + SO0036 0.58 -1.3 41,188 - SO0036 0.59 -0.8 41,216 + SO0036 0.64 -0.7 41,216 + SO0036 0.64 -0.0 41,360 + SO0036 0.90 -0.3 41,371 + -0.1 41,379 - -0.3 41,426 - -0.1 41,454 + -0.6 41,454 - -0.3 41,462 - -1.0 41,462 - -0.4 41,481 + +0.6 42,218 + -1.0 42,219 + -1.8 42,294 + hemF SO0038 0.16 -2.4 42,308 - hemF SO0038 0.17 -2.3 42,308 - hemF SO0038 0.17 -1.1 42,398 - hemF SO0038 0.27 -2.4 42,411 + hemF SO0038 0.28 -2.0
Or see this region's nucleotide sequence