Experiment: Chloramphenicol 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaB and hoxK are separated by 3 nucleotides hoxK and SO2100 are separated by 348 nucleotides SO2100 and SO2101 are separated by 3 nucleotides
SO2098: hyaB - quinone-reactive Ni/Fe hydrogenase, large subunit (NCBI ptt file), at 2,195,162 to 2,196,865
hyaB
SO2099: hoxK - quinone-reactive Ni/Fe hydrogenase, small subunit precursor (NCBI ptt file), at 2,196,869 to 2,198,005
hoxK
SO2100: SO2100 - thioredoxin family protein (NCBI ptt file), at 2,198,354 to 2,198,821
SO2100
SO2101: SO2101 - lipoprotein, putative (NCBI ptt file), at 2,198,825 to 2,199,046
SO2101
Position (kb)
2196
2197
2198
2199 Strain fitness (log2 ratio)
-1
0
1 at 2195.883 kb on + strand, within hyaB at 2195.891 kb on - strand, within hyaB at 2195.913 kb on - strand, within hyaB at 2196.063 kb on + strand, within hyaB at 2196.112 kb on + strand, within hyaB at 2196.167 kb on + strand, within hyaB at 2196.202 kb on + strand, within hyaB at 2196.280 kb on + strand, within hyaB at 2196.333 kb on - strand, within hyaB at 2196.389 kb on + strand, within hyaB at 2196.401 kb on + strand, within hyaB at 2196.403 kb on + strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.409 kb on - strand, within hyaB at 2196.411 kb on + strand, within hyaB at 2196.513 kb on + strand, within hyaB at 2196.516 kb on - strand, within hyaB at 2196.517 kb on + strand, within hyaB at 2196.540 kb on - strand, within hyaB at 2196.555 kb on - strand, within hyaB at 2196.577 kb on + strand, within hyaB at 2196.585 kb on - strand, within hyaB at 2196.585 kb on - strand, within hyaB at 2196.686 kb on - strand, within hyaB at 2196.715 kb on + strand at 2196.723 kb on - strand at 2196.736 kb on + strand at 2196.736 kb on + strand at 2196.783 kb on + strand at 2196.832 kb on + strand at 2196.959 kb on + strand at 2196.996 kb on - strand, within hoxK at 2197.039 kb on + strand, within hoxK at 2197.039 kb on + strand, within hoxK at 2197.047 kb on - strand, within hoxK at 2197.093 kb on - strand, within hoxK at 2197.163 kb on + strand, within hoxK at 2197.218 kb on - strand, within hoxK at 2197.390 kb on - strand, within hoxK at 2197.401 kb on + strand, within hoxK at 2197.401 kb on + strand, within hoxK at 2197.401 kb on + strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.409 kb on - strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.416 kb on + strand, within hoxK at 2197.508 kb on + strand, within hoxK at 2197.516 kb on - strand, within hoxK at 2197.775 kb on + strand, within hoxK at 2197.834 kb on + strand, within hoxK at 2197.947 kb on - strand at 2197.954 kb on + strand at 2197.996 kb on - strand at 2198.261 kb on - strand at 2198.388 kb on + strand at 2198.388 kb on + strand at 2198.506 kb on + strand, within SO2100 at 2198.506 kb on + strand, within SO2100 at 2198.681 kb on + strand, within SO2100 at 2198.792 kb on - strand at 2198.823 kb on - strand at 2198.894 kb on - strand, within SO2101 at 2199.003 kb on - strand, within SO2101
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0002 mg/ml remove 2,195,883 + hyaB SO2098 0.42 +0.1 2,195,891 - hyaB SO2098 0.43 +0.5 2,195,913 - hyaB SO2098 0.44 +0.3 2,196,063 + hyaB SO2098 0.53 +0.8 2,196,112 + hyaB SO2098 0.56 -0.1 2,196,167 + hyaB SO2098 0.59 +0.2 2,196,202 + hyaB SO2098 0.61 +0.4 2,196,280 + hyaB SO2098 0.66 -0.7 2,196,333 - hyaB SO2098 0.69 -0.3 2,196,389 + hyaB SO2098 0.72 +0.3 2,196,401 + hyaB SO2098 0.73 -0.2 2,196,403 + hyaB SO2098 0.73 +0.2 2,196,409 - hyaB SO2098 0.73 +0.5 2,196,409 - hyaB SO2098 0.73 +0.4 2,196,409 - hyaB SO2098 0.73 +0.1 2,196,409 - hyaB SO2098 0.73 +0.6 2,196,411 + hyaB SO2098 0.73 +0.4 2,196,513 + hyaB SO2098 0.79 -0.1 2,196,516 - hyaB SO2098 0.79 -1.1 2,196,517 + hyaB SO2098 0.80 -0.0 2,196,540 - hyaB SO2098 0.81 -0.2 2,196,555 - hyaB SO2098 0.82 -0.7 2,196,577 + hyaB SO2098 0.83 -1.6 2,196,585 - hyaB SO2098 0.84 -1.4 2,196,585 - hyaB SO2098 0.84 -0.0 2,196,686 - hyaB SO2098 0.89 +0.3 2,196,715 + -1.1 2,196,723 - -0.8 2,196,736 + -0.2 2,196,736 + -0.7 2,196,783 + -0.4 2,196,832 + -0.2 2,196,959 + -0.0 2,196,996 - hoxK SO2099 0.11 -0.3 2,197,039 + hoxK SO2099 0.15 +0.4 2,197,039 + hoxK SO2099 0.15 -0.2 2,197,047 - hoxK SO2099 0.16 -0.9 2,197,093 - hoxK SO2099 0.20 -0.0 2,197,163 + hoxK SO2099 0.26 -0.7 2,197,218 - hoxK SO2099 0.31 +0.3 2,197,390 - hoxK SO2099 0.46 +0.9 2,197,401 + hoxK SO2099 0.47 +0.6 2,197,401 + hoxK SO2099 0.47 -0.3 2,197,401 + hoxK SO2099 0.47 +0.4 2,197,409 - hoxK SO2099 0.47 -0.4 2,197,409 - hoxK SO2099 0.47 +0.7 2,197,409 - hoxK SO2099 0.47 +0.5 2,197,409 - hoxK SO2099 0.47 -0.1 2,197,409 - hoxK SO2099 0.47 -0.2 2,197,416 + hoxK SO2099 0.48 +0.3 2,197,416 + hoxK SO2099 0.48 -0.1 2,197,416 + hoxK SO2099 0.48 -0.7 2,197,416 + hoxK SO2099 0.48 -0.6 2,197,508 + hoxK SO2099 0.56 -1.4 2,197,516 - hoxK SO2099 0.57 -0.0 2,197,775 + hoxK SO2099 0.80 -0.1 2,197,834 + hoxK SO2099 0.85 -0.0 2,197,947 - -0.8 2,197,954 + -0.3 2,197,996 - -0.7 2,198,261 - +0.1 2,198,388 + +0.1 2,198,388 + +0.5 2,198,506 + SO2100 0.32 -0.3 2,198,506 + SO2100 0.32 +0.1 2,198,681 + SO2100 0.70 -0.3 2,198,792 - +0.6 2,198,823 - -0.3 2,198,894 - SO2101 0.31 +0.0 2,199,003 - SO2101 0.80 +0.4
Or see this region's nucleotide sequence