Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2095 and SO2096 overlap by 35 nucleotides SO2096 and hydC are separated by 7 nucleotides hydC and hyaB are separated by 21 nucleotides
SO2095: SO2095 - hypothetical protein (NCBI ptt file), at 2,192,061 to 2,193,914
SO2095
SO2096: SO2096 - hydrogenase expression/formation protein (NCBI ptt file), at 2,193,880 to 2,194,458
SO2096
SO2097: hydC - quinone-reactive Ni/Fe hydrogenase, cytochrome b subunit (NCBI ptt file), at 2,194,466 to 2,195,140
hydC
SO2098: hyaB - quinone-reactive Ni/Fe hydrogenase, large subunit (NCBI ptt file), at 2,195,162 to 2,196,865
hyaB
Position (kb)
2194
2195
2196 Strain fitness (log2 ratio)
-1
0
1 at 2193.511 kb on - strand, within SO2095 at 2193.736 kb on + strand at 2193.744 kb on - strand at 2193.863 kb on + strand at 2193.873 kb on + strand at 2193.930 kb on + strand at 2193.962 kb on - strand, within SO2096 at 2193.964 kb on + strand, within SO2096 at 2194.009 kb on + strand, within SO2096 at 2194.033 kb on + strand, within SO2096 at 2194.034 kb on - strand, within SO2096 at 2194.086 kb on + strand, within SO2096 at 2194.086 kb on + strand, within SO2096 at 2194.111 kb on + strand, within SO2096 at 2194.187 kb on - strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.347 kb on + strand, within SO2096 at 2194.355 kb on - strand, within SO2096 at 2194.540 kb on + strand, within hydC at 2194.657 kb on - strand, within hydC at 2194.657 kb on - strand, within hydC at 2194.679 kb on + strand, within hydC at 2194.679 kb on + strand, within hydC at 2194.741 kb on + strand, within hydC at 2194.749 kb on - strand, within hydC at 2194.749 kb on - strand, within hydC at 2195.061 kb on - strand, within hydC at 2195.068 kb on + strand, within hydC at 2195.224 kb on + strand at 2195.280 kb on - strand at 2195.323 kb on + strand at 2195.468 kb on - strand, within hyaB at 2195.587 kb on - strand, within hyaB at 2195.613 kb on - strand, within hyaB at 2195.614 kb on - strand, within hyaB at 2195.641 kb on - strand, within hyaB at 2195.792 kb on + strand, within hyaB at 2195.800 kb on - strand, within hyaB at 2195.840 kb on + strand, within hyaB at 2195.883 kb on + strand, within hyaB at 2195.891 kb on - strand, within hyaB at 2195.913 kb on - strand, within hyaB at 2196.063 kb on + strand, within hyaB at 2196.112 kb on + strand, within hyaB
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,193,511 - SO2095 0.78 +1.1 2,193,736 + -0.1 2,193,744 - +0.3 2,193,863 + -0.0 2,193,873 + -0.2 2,193,930 + -0.7 2,193,962 - SO2096 0.14 +0.2 2,193,964 + SO2096 0.15 +0.7 2,194,009 + SO2096 0.22 -0.5 2,194,033 + SO2096 0.26 +0.1 2,194,034 - SO2096 0.27 +0.4 2,194,086 + SO2096 0.36 +0.2 2,194,086 + SO2096 0.36 +0.1 2,194,111 + SO2096 0.40 -0.1 2,194,187 - SO2096 0.53 -0.2 2,194,347 + SO2096 0.81 -1.2 2,194,347 + SO2096 0.81 -0.1 2,194,347 + SO2096 0.81 -0.1 2,194,355 - SO2096 0.82 +0.5 2,194,540 + hydC SO2097 0.11 -0.5 2,194,657 - hydC SO2097 0.28 +0.1 2,194,657 - hydC SO2097 0.28 -0.2 2,194,679 + hydC SO2097 0.32 -0.6 2,194,679 + hydC SO2097 0.32 +0.4 2,194,741 + hydC SO2097 0.41 -1.6 2,194,749 - hydC SO2097 0.42 -0.3 2,194,749 - hydC SO2097 0.42 -0.2 2,195,061 - hydC SO2097 0.88 +0.6 2,195,068 + hydC SO2097 0.89 +0.2 2,195,224 + +0.3 2,195,280 - +1.1 2,195,323 + -0.3 2,195,468 - hyaB SO2098 0.18 -0.3 2,195,587 - hyaB SO2098 0.25 +0.0 2,195,613 - hyaB SO2098 0.26 +0.9 2,195,614 - hyaB SO2098 0.27 -0.1 2,195,641 - hyaB SO2098 0.28 +0.4 2,195,792 + hyaB SO2098 0.37 +0.3 2,195,800 - hyaB SO2098 0.37 +0.3 2,195,840 + hyaB SO2098 0.40 -0.1 2,195,883 + hyaB SO2098 0.42 -0.4 2,195,891 - hyaB SO2098 0.43 +0.1 2,195,913 - hyaB SO2098 0.44 -0.3 2,196,063 + hyaB SO2098 0.53 +0.8 2,196,112 + hyaB SO2098 0.56 +0.1
Or see this region's nucleotide sequence