Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1337 and lpxK are separated by 2 nucleotides lpxK and Sama_1339 overlap by 11 nucleotides Sama_1339 and Sama_1340 are separated by 262 nucleotides
Sama_1337: Sama_1337 - ABC transporter, ATP-binding protein MsbA (RefSeq), at 1,629,732 to 1,631,531
_1337
Sama_1338: lpxK - tetraacyldisaccharide 4'-kinase (RefSeq), at 1,631,534 to 1,632,583
lpxK
Sama_1339: Sama_1339 - hypothetical protein (RefSeq), at 1,632,573 to 1,632,758
_1339
Sama_1340: Sama_1340 - Mn2+-dependent serine/threonine protein kinase (RefSeq), at 1,633,021 to 1,633,770
_1340
Position (kb)
1631
1632
1633 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1632.764 kb on - strand at 1632.764 kb on - strand at 1632.902 kb on - strand at 1632.943 kb on + strand at 1632.943 kb on + strand at 1632.943 kb on + strand at 1632.945 kb on + strand at 1632.945 kb on + strand at 1632.945 kb on + strand at 1632.945 kb on + strand at 1632.945 kb on + strand at 1632.946 kb on - strand at 1632.946 kb on - strand at 1632.946 kb on - strand at 1632.950 kb on + strand at 1632.950 kb on + strand at 1632.950 kb on + strand at 1632.951 kb on - strand at 1632.951 kb on - strand at 1632.951 kb on - strand at 1632.951 kb on - strand at 1632.970 kb on + strand at 1632.989 kb on - strand at 1633.016 kb on + strand at 1633.017 kb on - strand at 1633.288 kb on + strand, within Sama_1340 at 1633.371 kb on + strand, within Sama_1340 at 1633.372 kb on - strand, within Sama_1340 at 1633.451 kb on + strand, within Sama_1340 at 1633.451 kb on + strand, within Sama_1340 at 1633.451 kb on + strand, within Sama_1340 at 1633.451 kb on + strand, within Sama_1340 at 1633.451 kb on + strand, within Sama_1340 at 1633.451 kb on + strand, within Sama_1340 at 1633.451 kb on + strand, within Sama_1340 at 1633.546 kb on + strand, within Sama_1340
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,632,764 - +0.5 1,632,764 - -0.2 1,632,902 - -0.3 1,632,943 + -0.1 1,632,943 + -0.3 1,632,943 + -0.2 1,632,945 + +0.9 1,632,945 + +0.3 1,632,945 + -0.4 1,632,945 + -0.9 1,632,945 + +0.2 1,632,946 - -0.1 1,632,946 - +0.5 1,632,946 - -1.7 1,632,950 + -0.2 1,632,950 + -2.1 1,632,950 + +0.3 1,632,951 - +0.4 1,632,951 - +0.4 1,632,951 - -0.9 1,632,951 - +0.2 1,632,970 + -0.7 1,632,989 - -0.7 1,633,016 + -0.7 1,633,017 - -1.9 1,633,288 + Sama_1340 0.36 -3.1 1,633,371 + Sama_1340 0.47 -1.2 1,633,372 - Sama_1340 0.47 -0.5 1,633,451 + Sama_1340 0.57 -1.9 1,633,451 + Sama_1340 0.57 -3.5 1,633,451 + Sama_1340 0.57 -1.9 1,633,451 + Sama_1340 0.57 -2.7 1,633,451 + Sama_1340 0.57 -0.1 1,633,451 + Sama_1340 0.57 -1.8 1,633,451 + Sama_1340 0.57 -2.1 1,633,546 + Sama_1340 0.70 -1.7
Or see this region's nucleotide sequence