Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and sucD overlap by 1 nucleotides sucD and rnk are separated by 377 nucleotides rnk and SO1936 are separated by 48 nucleotides
SO1932: sucC - succinyl-CoA synthase, beta subunit (NCBI ptt file), at 2,038,496 to 2,039,662
sucC
SO1933: sucD - succinyl-CoA synthase, alpha subunit (NCBI ptt file), at 2,039,662 to 2,040,534
sucD
SO1935: rnk - regulator of nucleoside diphosphate kinase (NCBI ptt file), at 2,040,912 to 2,041,319
rnk
SO1936: SO1936 - acetyltransferase, GNAT family (NCBI ptt file), at 2,041,368 to 2,041,865
SO1936
Position (kb)
2039
2040
2041 Strain fitness (log2 ratio)
-1
0
1 at 2039.289 kb on + strand, within sucC at 2040.665 kb on + strand at 2040.665 kb on + strand at 2040.673 kb on - strand at 2040.734 kb on + strand at 2040.734 kb on + strand at 2040.742 kb on - strand at 2040.742 kb on - strand at 2040.806 kb on + strand at 2040.806 kb on + strand at 2040.806 kb on + strand at 2040.877 kb on + strand at 2040.910 kb on - strand at 2040.968 kb on + strand, within rnk at 2041.020 kb on + strand, within rnk at 2041.335 kb on + strand at 2041.358 kb on - strand at 2041.410 kb on - strand at 2041.511 kb on + strand, within SO1936
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 2,039,289 + sucC SO1932 0.68 -0.7 2,040,665 + -0.1 2,040,665 + +0.5 2,040,673 - +0.5 2,040,734 + +0.2 2,040,734 + -0.7 2,040,742 - -1.0 2,040,742 - +0.4 2,040,806 + +0.6 2,040,806 + +0.0 2,040,806 + +0.5 2,040,877 + +0.8 2,040,910 - +0.3 2,040,968 + rnk SO1935 0.14 -0.8 2,041,020 + rnk SO1935 0.26 -1.3 2,041,335 + -0.1 2,041,358 - +0.7 2,041,410 - +0.4 2,041,511 + SO1936 0.29 +0.8
Or see this region's nucleotide sequence