Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1557 and phoB are separated by 155 nucleotides phoB and phoR are separated by 80 nucleotides phoR and SO1560 are separated by 225 nucleotides
SO1557: SO1557 - outer membrane porin, putative (NCBI ptt file), at 1,633,333 to 1,634,298
SO1557
SO1558: phoB - phosphate regulon response regulator PhoB (NCBI ptt file), at 1,634,454 to 1,635,173
phoB
SO1559: phoR - phosphate regulon sensor protein PhoR (NCBI ptt file), at 1,635,254 to 1,636,552
phoR
SO1560: SO1560 - phosphate-binding protein (NCBI ptt file), at 1,636,778 to 1,637,749
SO1560
Position (kb)
1635
1636
1637 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1634.292 kb on + strand at 1634.300 kb on - strand at 1634.300 kb on - strand at 1634.495 kb on - strand at 1634.568 kb on - strand, within phoB at 1634.613 kb on - strand, within phoB at 1634.613 kb on - strand, within phoB at 1634.615 kb on + strand, within phoB at 1634.615 kb on + strand, within phoB at 1634.615 kb on + strand, within phoB at 1634.623 kb on - strand, within phoB at 1634.623 kb on - strand, within phoB at 1634.623 kb on - strand, within phoB at 1634.623 kb on - strand, within phoB at 1634.648 kb on - strand, within phoB at 1634.756 kb on + strand, within phoB at 1634.763 kb on - strand, within phoB at 1634.764 kb on + strand, within phoB at 1634.814 kb on - strand, within phoB at 1634.846 kb on - strand, within phoB at 1634.925 kb on - strand, within phoB at 1635.227 kb on + strand at 1635.237 kb on + strand at 1635.449 kb on + strand, within phoR at 1635.449 kb on + strand, within phoR at 1635.449 kb on + strand, within phoR at 1635.563 kb on + strand, within phoR at 1635.563 kb on + strand, within phoR at 1635.569 kb on + strand, within phoR at 1635.571 kb on - strand, within phoR at 1635.571 kb on - strand, within phoR at 1635.592 kb on + strand, within phoR at 1635.592 kb on + strand, within phoR at 1635.595 kb on - strand, within phoR at 1635.605 kb on - strand, within phoR at 1635.605 kb on - strand, within phoR at 1635.619 kb on - strand, within phoR at 1635.643 kb on - strand, within phoR at 1635.674 kb on - strand, within phoR at 1635.810 kb on + strand, within phoR at 1635.846 kb on + strand, within phoR at 1636.021 kb on + strand, within phoR at 1636.021 kb on + strand, within phoR at 1636.021 kb on + strand, within phoR at 1636.021 kb on + strand, within phoR at 1636.029 kb on - strand, within phoR at 1636.105 kb on + strand, within phoR at 1636.138 kb on - strand, within phoR at 1636.138 kb on - strand, within phoR at 1636.155 kb on - strand, within phoR at 1636.210 kb on + strand, within phoR at 1636.210 kb on + strand, within phoR at 1636.218 kb on - strand, within phoR at 1636.218 kb on - strand, within phoR at 1636.218 kb on - strand, within phoR at 1636.261 kb on + strand, within phoR at 1636.309 kb on - strand, within phoR at 1636.331 kb on + strand, within phoR at 1636.331 kb on + strand, within phoR at 1636.338 kb on + strand, within phoR at 1636.427 kb on + strand at 1636.435 kb on - strand at 1636.449 kb on + strand at 1636.463 kb on - strand at 1636.505 kb on + strand at 1636.505 kb on + strand at 1636.536 kb on - strand at 1636.600 kb on - strand at 1636.726 kb on + strand at 1636.823 kb on + strand at 1636.823 kb on + strand at 1636.823 kb on + strand at 1636.831 kb on - strand at 1636.831 kb on - strand at 1636.836 kb on - strand at 1636.861 kb on + strand at 1636.911 kb on - strand, within SO1560 at 1636.977 kb on - strand, within SO1560 at 1636.977 kb on - strand, within SO1560 at 1637.070 kb on + strand, within SO1560 at 1637.227 kb on - strand, within SO1560 at 1637.273 kb on - strand, within SO1560 at 1637.381 kb on - strand, within SO1560 at 1637.388 kb on - strand, within SO1560
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 1,634,292 + +0.6 1,634,300 - +0.1 1,634,300 - -0.0 1,634,495 - -0.0 1,634,568 - phoB SO1558 0.16 -0.5 1,634,613 - phoB SO1558 0.22 -0.0 1,634,613 - phoB SO1558 0.22 +0.1 1,634,615 + phoB SO1558 0.22 +0.4 1,634,615 + phoB SO1558 0.22 +0.3 1,634,615 + phoB SO1558 0.22 +0.7 1,634,623 - phoB SO1558 0.23 +1.0 1,634,623 - phoB SO1558 0.23 +0.4 1,634,623 - phoB SO1558 0.23 -0.1 1,634,623 - phoB SO1558 0.23 +1.0 1,634,648 - phoB SO1558 0.27 -0.2 1,634,756 + phoB SO1558 0.42 +0.1 1,634,763 - phoB SO1558 0.43 -0.1 1,634,764 + phoB SO1558 0.43 -0.2 1,634,814 - phoB SO1558 0.50 +0.3 1,634,846 - phoB SO1558 0.54 +0.0 1,634,925 - phoB SO1558 0.65 +0.6 1,635,227 + +0.9 1,635,237 + -0.4 1,635,449 + phoR SO1559 0.15 -0.6 1,635,449 + phoR SO1559 0.15 +0.2 1,635,449 + phoR SO1559 0.15 +0.2 1,635,563 + phoR SO1559 0.24 -0.6 1,635,563 + phoR SO1559 0.24 -0.6 1,635,569 + phoR SO1559 0.24 +0.7 1,635,571 - phoR SO1559 0.24 -0.4 1,635,571 - phoR SO1559 0.24 +0.2 1,635,592 + phoR SO1559 0.26 -0.3 1,635,592 + phoR SO1559 0.26 +1.9 1,635,595 - phoR SO1559 0.26 -0.4 1,635,605 - phoR SO1559 0.27 -0.2 1,635,605 - phoR SO1559 0.27 +0.2 1,635,619 - phoR SO1559 0.28 +0.3 1,635,643 - phoR SO1559 0.30 +0.1 1,635,674 - phoR SO1559 0.32 +0.2 1,635,810 + phoR SO1559 0.43 -0.7 1,635,846 + phoR SO1559 0.46 -0.7 1,636,021 + phoR SO1559 0.59 -0.0 1,636,021 + phoR SO1559 0.59 +0.0 1,636,021 + phoR SO1559 0.59 +0.0 1,636,021 + phoR SO1559 0.59 +0.2 1,636,029 - phoR SO1559 0.60 +0.5 1,636,105 + phoR SO1559 0.66 +0.3 1,636,138 - phoR SO1559 0.68 +0.1 1,636,138 - phoR SO1559 0.68 -1.8 1,636,155 - phoR SO1559 0.69 -1.3 1,636,210 + phoR SO1559 0.74 -0.2 1,636,210 + phoR SO1559 0.74 +0.3 1,636,218 - phoR SO1559 0.74 +0.5 1,636,218 - phoR SO1559 0.74 +0.2 1,636,218 - phoR SO1559 0.74 +0.2 1,636,261 + phoR SO1559 0.78 +0.3 1,636,309 - phoR SO1559 0.81 +0.4 1,636,331 + phoR SO1559 0.83 -0.8 1,636,331 + phoR SO1559 0.83 -0.8 1,636,338 + phoR SO1559 0.83 +0.5 1,636,427 + -0.1 1,636,435 - +0.5 1,636,449 + +0.7 1,636,463 - -0.4 1,636,505 + +0.0 1,636,505 + +0.1 1,636,536 - -0.1 1,636,600 - +1.7 1,636,726 + +0.3 1,636,823 + -0.7 1,636,823 + +0.3 1,636,823 + +0.1 1,636,831 - +0.1 1,636,831 - -0.1 1,636,836 - +0.5 1,636,861 + -0.2 1,636,911 - SO1560 0.14 +0.4 1,636,977 - SO1560 0.20 -1.2 1,636,977 - SO1560 0.20 -1.4 1,637,070 + SO1560 0.30 +0.3 1,637,227 - SO1560 0.46 +0.3 1,637,273 - SO1560 0.51 +0.9 1,637,381 - SO1560 0.62 -0.1 1,637,388 - SO1560 0.63 -0.0
Or see this region's nucleotide sequence