Experiment: D-Cycloserine 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1853 and Sama_1854 are separated by 5 nucleotides Sama_1854 and thiH are separated by 114 nucleotides
Sama_1853: Sama_1853 - hypothetical protein (RefSeq), at 2,257,149 to 2,258,933
_1853
Sama_1854: Sama_1854 - methyl-accepting chemotaxis protein (RefSeq), at 2,258,939 to 2,259,190
_1854
Sama_1855: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,259,305 to 2,260,420
thiH
Position (kb)
2258
2259
2260 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2257.968 kb on + strand, within Sama_1853 at 2258.195 kb on + strand, within Sama_1853 at 2258.196 kb on - strand, within Sama_1853 at 2258.196 kb on - strand, within Sama_1853 at 2258.196 kb on - strand, within Sama_1853 at 2258.202 kb on - strand, within Sama_1853 at 2258.309 kb on + strand, within Sama_1853 at 2258.310 kb on - strand, within Sama_1853 at 2258.310 kb on - strand, within Sama_1853 at 2258.346 kb on - strand, within Sama_1853 at 2258.346 kb on - strand, within Sama_1853 at 2258.456 kb on + strand, within Sama_1853 at 2258.634 kb on + strand, within Sama_1853 at 2258.634 kb on + strand, within Sama_1853 at 2258.635 kb on - strand, within Sama_1853 at 2258.638 kb on - strand, within Sama_1853 at 2258.667 kb on + strand, within Sama_1853 at 2258.668 kb on - strand, within Sama_1853 at 2258.811 kb on + strand at 2258.812 kb on - strand at 2258.940 kb on + strand at 2258.941 kb on - strand at 2258.954 kb on + strand at 2258.954 kb on + strand at 2258.954 kb on + strand at 2258.954 kb on + strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2258.955 kb on - strand at 2259.053 kb on - strand, within Sama_1854 at 2259.206 kb on - strand at 2259.206 kb on - strand at 2259.283 kb on - strand at 2259.307 kb on - strand at 2259.340 kb on + strand at 2259.340 kb on + strand at 2259.340 kb on + strand at 2259.341 kb on - strand at 2259.432 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.448 kb on + strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.449 kb on - strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.568 kb on + strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.569 kb on - strand, within thiH at 2259.587 kb on - strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.649 kb on + strand, within thiH at 2259.684 kb on + strand, within thiH at 2259.793 kb on + strand, within thiH at 2259.809 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.847 kb on - strand, within thiH at 2259.862 kb on - strand, within thiH at 2259.955 kb on - strand, within thiH at 2259.988 kb on + strand, within thiH at 2260.051 kb on - strand, within thiH at 2260.085 kb on - strand, within thiH at 2260.115 kb on - strand, within thiH at 2260.143 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.176 kb on + strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.177 kb on - strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.188 kb on + strand, within thiH at 2260.189 kb on - strand, within thiH at 2260.189 kb on - strand, within thiH
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.03125 mg/ml remove 2,257,968 + Sama_1853 0.46 -0.9 2,258,195 + Sama_1853 0.59 +0.6 2,258,196 - Sama_1853 0.59 -0.4 2,258,196 - Sama_1853 0.59 +0.1 2,258,196 - Sama_1853 0.59 +1.3 2,258,202 - Sama_1853 0.59 -1.7 2,258,309 + Sama_1853 0.65 -0.1 2,258,310 - Sama_1853 0.65 +0.1 2,258,310 - Sama_1853 0.65 -0.3 2,258,346 - Sama_1853 0.67 -1.5 2,258,346 - Sama_1853 0.67 +1.1 2,258,456 + Sama_1853 0.73 +0.3 2,258,634 + Sama_1853 0.83 -1.7 2,258,634 + Sama_1853 0.83 -0.9 2,258,635 - Sama_1853 0.83 -0.9 2,258,638 - Sama_1853 0.83 +1.0 2,258,667 + Sama_1853 0.85 +0.5 2,258,668 - Sama_1853 0.85 -0.2 2,258,811 + -0.7 2,258,812 - +0.5 2,258,940 + -0.9 2,258,941 - +1.5 2,258,954 + +0.5 2,258,954 + +0.4 2,258,954 + -0.3 2,258,954 + -0.4 2,258,955 - -0.7 2,258,955 - -0.3 2,258,955 - -0.6 2,258,955 - +1.2 2,258,955 - +0.2 2,259,053 - Sama_1854 0.45 -0.0 2,259,206 - -0.3 2,259,206 - -0.2 2,259,283 - -0.7 2,259,307 - +0.2 2,259,340 + -0.9 2,259,340 + -0.0 2,259,340 + +0.3 2,259,341 - +0.0 2,259,432 + thiH Sama_1855 0.11 -0.2 2,259,448 + thiH Sama_1855 0.13 -2.1 2,259,448 + thiH Sama_1855 0.13 +0.6 2,259,448 + thiH Sama_1855 0.13 -2.0 2,259,448 + thiH Sama_1855 0.13 +0.3 2,259,448 + thiH Sama_1855 0.13 -0.2 2,259,449 - thiH Sama_1855 0.13 -0.2 2,259,449 - thiH Sama_1855 0.13 -1.4 2,259,449 - thiH Sama_1855 0.13 +0.6 2,259,449 - thiH Sama_1855 0.13 +1.8 2,259,449 - thiH Sama_1855 0.13 -1.3 2,259,568 + thiH Sama_1855 0.24 -0.2 2,259,568 + thiH Sama_1855 0.24 +1.3 2,259,569 - thiH Sama_1855 0.24 -2.1 2,259,569 - thiH Sama_1855 0.24 -0.3 2,259,569 - thiH Sama_1855 0.24 -0.8 2,259,569 - thiH Sama_1855 0.24 -1.5 2,259,569 - thiH Sama_1855 0.24 +0.3 2,259,587 - thiH Sama_1855 0.25 -1.7 2,259,649 + thiH Sama_1855 0.31 +1.1 2,259,649 + thiH Sama_1855 0.31 -0.3 2,259,684 + thiH Sama_1855 0.34 +2.3 2,259,793 + thiH Sama_1855 0.44 -0.9 2,259,809 - thiH Sama_1855 0.45 +1.3 2,259,847 - thiH Sama_1855 0.49 +1.1 2,259,847 - thiH Sama_1855 0.49 +0.3 2,259,847 - thiH Sama_1855 0.49 +0.8 2,259,847 - thiH Sama_1855 0.49 -0.1 2,259,862 - thiH Sama_1855 0.50 +0.4 2,259,955 - thiH Sama_1855 0.58 -0.0 2,259,988 + thiH Sama_1855 0.61 -0.4 2,260,051 - thiH Sama_1855 0.67 -2.4 2,260,085 - thiH Sama_1855 0.70 -0.0 2,260,115 - thiH Sama_1855 0.73 +0.3 2,260,143 + thiH Sama_1855 0.75 +1.3 2,260,176 + thiH Sama_1855 0.78 +0.3 2,260,176 + thiH Sama_1855 0.78 +0.3 2,260,176 + thiH Sama_1855 0.78 -0.2 2,260,177 - thiH Sama_1855 0.78 +0.2 2,260,177 - thiH Sama_1855 0.78 -0.3 2,260,188 + thiH Sama_1855 0.79 -1.2 2,260,188 + thiH Sama_1855 0.79 +0.8 2,260,188 + thiH Sama_1855 0.79 +0.5 2,260,189 - thiH Sama_1855 0.79 -0.7 2,260,189 - thiH Sama_1855 0.79 -1.7
Or see this region's nucleotide sequence